Bluegene Biotech http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech BlueGene Biotech is a professional ELISA kits manufacturer with 12 years of experience, which focuses on life sciences products and biotechnology. https://www.elisakit.cc/ ja-JP Hamster ELISA Kits(2024年04月18日) <p><a href="https://www.elisakit.cc/products/hamster-elisa-kits/">https://www.elisakit.cc/products/hamster-elisa-kits/</a></p> <p>Hamster ELISA Kits<br /> A hamster is also known as suslik, which is an animal domesticated by wild animals and entered the laboratory. There are 8 kinds of hamsters for experimental research. There are mainly two kinds of hamsters as experimental animals: Golden hamster, Mesometritus auratus and Chinese hamster, Crucetulus griseus. Among them, the Chinese hamsters are widely used in nutrition and infectious disease research. In addition, the Chinese hamsters are often used for the study of diabetes, and they are good animal models of true diabetes.</p> <p>How to Distinguish Rats, Mice, Guinea Pigs, Hamsters?<br /> Rats are one of the rodents. They are larger than mice and are characterized by blunt mouths and long and thick tails. The two most common rat species include black rats and brown rats. 21 chromosomes, the pups mature within 6-8 weeks. The mouse evolved from the house mouse. It is small. The experimental mouse has a tail and 20 pairs of chromosomes. The pups mature within 9-13 weeks. Guinea pigs are larger in rodents, weighing around 700 to 1200g, with a body length of 20 to 25cm and no tail. The head is large, which size is one-third of the body. The eyes are big and round, and the ears are short and close to the head. The hamster is short and thick, weighing 30 to 1000 grams. The tail is 7 to 106 mm long, usually half the body length, scaleless, and covered with thick hair.</p> <p>What Are the Advantages of Hamsters As Experimental Animals?<br /> The Chinese hamster is an experimental animal with Chinese characteristics, which plays an important role in medical and biological research. For example, Meier conducted pathological studies on various organs of Chinese hamsters with spontaneous and hereditary diabetes. Shuhua Sun found that the sugar metabolism and protein metabolism were abnormal in the spontaneous T2DM Chinese hamster study of blood electrolyte content, blood glucose metabolism index content, six indexes of blood-related enzymes, and total protein content. It shows that the Chinese hamster is an ideal animal model. It is widely used in life science research such as genetics, infectious disease, and tissue culture, especially in animal models and mechanism research such as diabetes and oral cancer.</p> <p>As a professional drug residue test kit factory, Shanghai bluegene biotech has high-quality human insulin elisa kit for sale. Welcome to contact us if needed.<br /> <a href="https://www.elisakit.cc/uploads/image/20230117/10/ldh-elisa-kit.jpg" rel="nofollow"><img src="https://www.elisakit.cc/uploads/image/20230117/10/ldh-elisa-kit.jpg" alt="ldh-elisa-kit.jpg" /></a></p> 2024-04-18T18:21:25+0900 http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/zu3uzOaFOih BluegeneBiotech http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/zu3uzOaFOih#comments http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/zu3uzOaFOih Growth Factor Proteins(2024年04月18日) <p><a href="https://www.elisakit.cc/products/growth-factor-proteins/">https://www.elisakit.cc/products/growth-factor-proteins/</a></p> <p>Growth factors are a kind of soluble signal molecules, which are necessary to regulate the normal growth and metabolism of microorganisms, but they are organic compounds that cannot be synthesized by themselves with simple carbon and nitrogen sources. They are usually secreted proteins or steroids, which can stimulate cell proliferation, migration, differentiation and multicellular morphogenesis during development and tissue healing.</p> <p>In a broad sense, besides vitamins, growth factor for sale also include bases, purines, pyrimidines, biotins, niacin, etc., and sometimes amino acids required by amino acid-deficient mutants. In the narrow sense, fibroblast growth factor 10 generally refer to vitamins. A kind of polypeptide that can regulate cell growth and other cell functions by binding to specific, high-affinity cell membrane receptors.</p> <p>Growth Factor Proteins FAQs<br /> Q<br /> What is growth factor protein?<br /> fibroblast growth factor 5 are necessary to regulate the normal growth and metabolism of microorganisms, but they cannot be synthesized by themselves with simple carbon and nitrogen sources. In a broad sense, it includes vitamins, bases, purines, pyrimidines, biotins, niacin, etc. Generally, growth factors are peptide hormones, including insulin, epidermal growth factor (EGF), fibroblast growth factor (FGF), platelet-derived growth factor (PDGF), and somatostatin released by growth hormon (somatostatin=SRIH). It is a kind of polypeptide substance with multiple effects such as regulating cell growth and other cell functions by binding to specific, high-affinity cell membrane receptors. It exists in platelets, various adult and embryonic tissues and most cultured cells, and has certain specificity for different types of cells. Generally, the growth of cultured cells requires the coordination of multiple fibroblast growth factor 19. Tumor cells have the characteristics of independent fibroblast growth factor 3.</p> <p>Q<br /> Growth factor proteins function<br /> fibroblast growth factor 8 usually have the functions of stimulating cell proliferation, damage repair and cell differentiation. It is a secreted protein or hormone that plays an important role in regulating intracellular processes. It is an important signal molecule between cells. For example, cytokines and hormones can bind to specific receptors on the surface of target cells.</p> <p>For more information about growth factor price and bluegene elisa kit, please feel free to contact us!</p> <p>If you are looking for a reliable growth factor company, don't hesitate to contact us!<a href="https://bluegenebiotech.up.seesaa.net/image/6-1-thumbnail2.jpg" rel="nofollow"><img src="https://bluegenebiotech.up.seesaa.net/image/6-1-thumbnail2.jpg" alt="6-1-thumbnail2.jpg" /></a></p> 2024-04-18T18:20:26+0900 http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/0CIaBN2HRca BluegeneBiotech http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/0CIaBN2HRca#comments http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/0CIaBN2HRca Fish ELISA Kits(2024年04月18日) <p><a href="https://www.elisakit.cc/products/fish-elisa-kits/">https://www.elisakit.cc/products/fish-elisa-kits/</a></p> <p>Fish ELISA Kits<br /> Fish are often used in environmental monitoring, embryo research, tumor research and others. Among them, zebrafish is the most commonly used. The zebrafish is a common tropical fish, which is native to South Asia. The zebrafish have a slender body with an adult length of 3~4cm, and they do not require high water quality. About 3 months after hatching, the fish reach sexual maturity, and mature fish can lay eggs every few days. The eggs are fertilized in vitro and develop in vitro. The embryo development is synchronous and fast, and the embryo body is transparent.</p> <p>Why Can Zebrafish Be Used As a Sample Model to Study Human Related Diseases?<br /> Zebrafish have a high similarity of 87% with human genes, and their regeneration ability is very strong, so they are used in both retinal repair and auditory repair. In immunology, adult zebrafish can survive without the generation of thymus and lymphocytes. The zebrafish in early embryonic development after Escherichia coli infection is locally cleared by macrophages, which suggests that there may also be a cytokine or chemokine system similar to that of mammals in zebrafish. In addition, zebrafish, as a model organism, their development, function, and diseases (such as nervous system, immune system, cardiovascular system, reproductive system, etc.) in various systems (such as neurodegenerative diseases, hereditary cardiovascular diseases, diabetes, etc.) are deeply studied.</p> <p>What Are the Precautions when Taking Fish Samples?<br /> Taking zebrafish as an example, they live in water temperatures between 20℃ and 23 ℃. Feed Artemia regularly one week before the experiment and observe the clarity of the water in the water tank in time. The zebrafish has a strong circadian rhythm. And it is necessary to pay attention to switching on the light at 8 am and off at 10 pm. Due to the small body sizes, they should be soft to hold during the operation. After the operation, it should be treated with ice-freezing anesthesia. In addition, zebrafish have less blood with the blood color light blue or colorless. In the immunoassay, there are a few body fluid samples, which can be cut into pieces, broken and mixed by the homogenizer, and then centrifuged to take supernatant samples.</p> <p>For more information about fish cortisol elisa kit, please feel free to contact us!</p> <p>As a professional drug residue test kit factory, Bluegene elisa has high-quality bovine igg elisa kits for sale. Welcome to contact us if needed.<br /> <a href="https://www.elisakit.cc/uploads/image/20230117/10/lam-elisa-kit.jpg" rel="nofollow"><img src="https://www.elisakit.cc/uploads/image/20230117/10/lam-elisa-kit.jpg" alt="lam-elisa-kit.jpg" /></a></p> 2024-04-18T18:19:53+0900 http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/bOlrlX1r5Wp BluegeneBiotech http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/bOlrlX1r5Wp#comments http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/bOlrlX1r5Wp Expression Plasmids(2024年04月18日) <p><a href="https://www.elisakit.cc/products/expression-plasmids/">https://www.elisakit.cc/products/expression-plasmids/</a></p> <p>cre expression plasmid are a kind of double-stranded, closed-loop DNA with a size range from 1kb to more than 200kb. All kinds of antibody expression plasmid are self-replicating genetic components that keep existing in the cytoplasm and can be independent of the cell chromosome.</p> <p>Generally, they are in a free state outside the chromosome. But under certain conditions, they can also be reversibly integrated into the host chromosome. It replicates with the replication of chromosomes and then it can be transmitted to offspring through cell division. At present, There are many methods to be used to extract plasmid DNA. The method of alkaline lysis is used to extract plasmid DNA.</p> <p>Expression Plasmids FAQs<br /> Q<br /> How are plasmids expressed?<br /> Genetic tools express genes in cells and are usually in the form of e coli expression plasmid or viruses. These vectors are generally used to introduce specific genes into target cells, and use the protein production system in target cells to express the protein encoded by genes on the vectors. This is the basic tool for protein production in biotechnology. It usually contains some regulatory sequences, such as enhancers and promoters, to improve the expression efficiency of genes carried on them. A good expression vector should be able to efficiently produce its encoded protein, which is generally achieved by transcribing a large number of stable messenger RNAs to translate them into target proteins. The production of the target protein can also be strictly regulated so that it can only be mass-produced when there are specific induction conditions.</p> <p>Q<br /> What is the difference between cloning and expression eukaryotic expression plasmid?<br /> Cloning vectors and expression vectors are two types of vectors, used in recombinant DNA technology to transfer foreign DNA chain segments into a target cell. Both cloning and expression vectors comprise replication origin, multiple cloning sites, and selectable marker genes in their vector sequences. Both cloning and expression vectors are self-replicative due to the presence of a replication origin. Cloning vectors can be either mirna expression plasmid, cosmids, or bacteriophages. The main difference between a cloning vector and an expression vector is that a cloning vector is used to transfer foreign DNA chain segments into a host cell, whereas an expression vector is a type of cloning vector, which contains suitable expression signals, promoter,ribosome-binding site, stop code with maximal gene expression.</p> <p>As a professional drug residue test kit factory, Bluegene elisa has high-quality drug residue test kit for sale. Welcome to contact us if needed.<a href="https://bluegenebiotech.up.seesaa.net/image/4-1-aa6a9-thumbnail2.jpg" rel="nofollow"><img src="https://bluegenebiotech.up.seesaa.net/image/4-1-aa6a9-thumbnail2.jpg" alt="4-1-aa6a9-thumbnail2.jpg" /></a></p> 2024-04-18T18:19:22+0900 http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/AZAKMRePJD2 BluegeneBiotech http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/AZAKMRePJD2#comments http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/AZAKMRePJD2 ELISA Kits(2024年04月18日) <p><a href="https://www.elisakit.cc/products/elisa-kits/">https://www.elisakit.cc/products/elisa-kits/</a></p> <p>The elisa kit company Shanghai BlueGene Biotech has focused on the research and development of ELISA kits, especially for more than a decade.</p> <p>Our ELISA Kit is a quick, convenient, and accurate research tool for the detection and quantification of targets in various samples.</p> <p>BlueGene Biotech's ELISA kits are 1.5-hour solid-phase testosterone elisa kit, the operation is much simpler. The ELISA kits are equipped with prediluted standards to avoid the possible operational errors. Our lab performs the quality tests ahead to assure the accuracy of the standards from each batch, and then ensure a reliable standard curve. The incubation and washing steps are also easy to operate, which enables the experiment finished within 1.5 hours. We believe that fewer handling steps can reduce errors, therefore, deliver more consistent results.</p> <p>With a mature and high-tech manufacturing workshop, we are able to provide a wide range of ELISA kits but with a very short lead time and reasonable price and elisa kit cost. You may search for the products you need in the search column, or find our contact information, BlueGene Biotech team will reply to you as soon as possible.</p> <p>Why Choose BlueGene Biotech ELISA Kits?<br /> Simple operation (1.5-hour solid-phase ELISA): This is a very simple and easy procedure. In addition, our kits do not need to put the plate on an orbital microplate shaker during the 2 incubation steps.</p> <p>Prediluted standards (ready-to-use standards): The concentration of the standards is critical for an accurate standard curve. Therefore this kit contains serial dilutions of the standards rather than a concentrated standard solution, thereby avoiding pipetting or calculation errors. Quality tests assure that the concentration of the standards is accurate, thereby ensuring a reliable standard curve.</p> <p>Reliability: Simple incubation and washing steps enable the Elisa experiment performed within 1.5 hours. Compared to the traditional Elisa method, fewer handling steps reduce errors and deliver more consistent results. Thorough and regular tests of the system guarantee a great intra and inter-assay reliability and ensure a low coefficient of variation.</p> <p>The types of sample (both of the two types of samples can be tested using one kit)</p> <p>Sample I: serum or plasma</p> <p>Sample II: cell culture supernatants, body fluid, and tissue homogenate</p> <p>How To Use ELISA Kits?<br /> BlueGene Biotech: One-Step Competitive ELISA kit</p> <p>1) Add 100 ul Standards or Samples to the well. Add 100 ul PBS (pH 7.0-7.2) in the blank control well.</p> <p>2) Dispense 10 ul Balance Solution into 100 ul samples, mix well. (NOTE: This step should be skipped for serum or plasma samples )</p> <p>3) Add 50 ul Conjugate to each well (NOT blank control well). Cover and incubate the plate for 1 hour at 37℃.</p> <p>4) Wash the microtiter plate.</p> <p>BlueGene Biotech: One-Step Competitive ELISA kit<br /> BlueGene Biotech: One-Step Sandwich ELISA</p> <p>1) Add 50 ul Standards or Samples to the well. Add 50 uL PBS (pH 7.0-7.2) in the blank control well.</p> <p>2) Dispense 5 uL Balance Solution into 50 uL samples only, mix well. (NOTE: This step should be skipped for serum or plasma samples )</p> <p>3) Add 100 uL Conjugate to each well (NOT blank control well). Mix well. Cover and incubate the plate for 1 hour at 37℃.</p> <p>4) Wash the microtiter plate.</p> <p>BlueGene Biotech: One-Step Sandwich ELISA<br /> Cgbio® ELISA kits-Reaearch Reagent: two-Step Sandwich ELISA</p> <p>1) Place all kits’ components at room temperature for 30mins before using.</p> <p>2) Prepare Wash Solution</p> <p>3) Prepare standards, then add 100ul diluted standards or samples to appropriate wells.</p> <p>4) Prepare Detection Antibody, then add 50ul to each wells, cover the plate and incubate for 120 mins at room temperature.</p> <p>5) Washing Step</p> <p>6) Prepare HRP-Streptavidin, then add 100ul to each well, cover the plate and incubate for 45 mins at room temperature.</p> <p>Cgbio® ELISA kits-Reaearch Reagent: two-Step Sandwich ELISA<br /> Cgbio® ELISA kits-Reaearch Reagent: three-Step Sandwich ELISA</p> <p>1) Place all kits’ components at room temperature for 30mins before using.</p> <p>2) Prepare Wash Solution (1x)</p> <p>3) Prepare Standards,then add 100ul of diluted standards or samples to appropriate wells.</p> <p>4) Cover the plate and incubate for 120 mins at room temperature.</p> <p>5) Washing Step</p> <p>6) Prepare Detection Antibody, then add 100ul to each wells, cover the plate and incubate for 60 mins at room temperature.</p> <p>7) Washing Step</p> <p>8) Prepare HRP-Streptavidin, then add 100ul to each well, cover the plate and incubate for 45 mins at room temperature.</p> <p>9) Washing Step</p> <p>10) Add 100ul TMB substrate to each well. Incubate at room temperature for 10 to 15 mins.</p> <p>Cgbio® ELISA kits-Reaearch Reagent: three-Step Sandwich ELISA<br /> Cgbio® ELISA kits-Drug Residue Test kit: three-Step Sandwich ELISA</p> <p>1) Place all kits’ components at room temperature for 30mins before using.</p> <p>2) Prepare Wash Solution (1x)</p> <p>3) Add 100ul of diluted standards or samples to appropriate wells.</p> <p>4) Cover the plate with a sealer, and incubate for 90 mins at 37℃.</p> <p>5) Washing Step</p> <p>6) Prepare Detection Antibody, then add 100ul to each wells, cover the plate and incubate for 60 mins at room temperature.</p> <p>7) Washing Step</p> <p>8) Prepare HRP-Streptavidin, then add 100ul to each well, cover the plate and incubate for 45 mins at room temperature.</p> <p>9) Washing Step</p> <p>10) Add 100ul TMB substrate to each well. Incubate at room temperature for 10 to 15 mins.</p> <p>11) Add 50ul Stop Solution to each well to stop the reaction.</p> <p>12) Run the microplate reader and conduct measurement at 450nm.</p> <p>13) Data analysis: Four parameter curve fitting is recommended.</p> <p>Cgbio® ELISA kits-Drug Residue Test kit: two-Step Sandwich ELISA</p> <p>1) Place all kits’ components at room temperature for 30mins before using.</p> <p>2) Prepare Wash Solution (1x)</p> <p>3) Add 100ul of diluted standards or samples to appropriate wells.</p> <p>4) Cover the plate with a sealer, and incubate for 90 mins at 37℃.</p> <p>5) Washing Step</p> <p>6) Dilute HRP-conjugated antibody to reach its working concentration (1x). Then add 100ul to each wells, cover the plate with a sealer, and incubate for 90 mins at 37℃.</p> <p>7) Washing Step</p> <p>For more information about pathology lab reagents list, please feel free to contact us!<br /> <a href="https://www.elisakit.cc/uploads/image/20221101/10/mouse-elisa-kits.jpg" rel="nofollow"><img src="https://www.elisakit.cc/uploads/image/20221101/10/mouse-elisa-kits.jpg" alt="mouse-elisa-kits.jpg" /></a></p> 2024-04-18T18:18:44+0900 http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/ApPhAGfnbYR BluegeneBiotech http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/ApPhAGfnbYR#comments http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/ApPhAGfnbYR Hamster Total Adiponectin ELISA kit(2024年04月18日) <p><a href="https://www.elisakit.cc/products/e18a0125-hamster-adpn-total-adiponectin-elisa-kits/">https://www.elisakit.cc/products/e18a0125-hamster-adpn-total-adiponectin-elisa-kits/</a></p> <p>E18A0125 Hamster Total Adiponectin ELISA kit</p> <p>Hamster ELISA kit is suitable for the detection of samples from Hamster species. Total Adiponectin can also be called 30 kDa adipocyte complement related protein, 30 kDa adipocyte complement-related protein, ACDC, ACRP 30, ACRP30, ADIPO, Adipocyte, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related 30 kDa protein, Adipocyte complement related protein of 30 kDa, Adipocyte complement-related 30 kDa protein, Adiponectin, AdipoQ, Adipose most abundant gene transcript 1, Adipose most abundant gene transcript 1 protein, Adipose specific collagen like factor, ADIPQTL1, ADPN, APM 1, apM-1, ApM1, C1q and collagen domain-containing protein, GBP 28, GBP28, Gelatin binding protein, Gelatin binding protein 28, Gelatin-binding protein.</p> <p>Principle of the Assay</p> <p>ADPN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ADPN antibody and an ADPN-HRP conjugate. The assay sample and buffer are incubated together with ADPN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADPN concentration since ADPN from samples and ADPN-HRP conjugate compete for the anti-ADPN antibody binding site. Since the number of sites is limited, as more sites are occupied by ADPN from the sample, fewer sites are left to bind ADPN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADPN concentration in each sample is interpolated from this standard curve.</p> <p>Citations of Hamster ADPN Total Adiponectin ELISA kit<br /> E18A0125 has been referenced in the below publications:</p> <p>Effects of different amounts and types of dietary fatty acids on the body weight, fat accumulation, and lipid metabolism in hamsters.</p> <p>As a professional drug residue test kit factory, Shanghai bluegene biotech has high-quality human insulin elisa kit for sale. Welcome to contact us if needed.<br /> <a href="https://bluegenebiotech.up.seesaa.net/image/2-1-00a1a-thumbnail2.jpg" rel="nofollow"><img src="https://bluegenebiotech.up.seesaa.net/image/2-1-00a1a-thumbnail2.jpg" alt="2-1-00a1a-thumbnail2.jpg" /></a></p> 2024-04-18T18:18:09+0900 http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/6f2qqVq1Bey BluegeneBiotech http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/6f2qqVq1Bey#comments http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/6f2qqVq1Bey Fish Cyclic Adenosine Monophosphate ELISA kit(2024年04月18日) <p><a href="https://www.elisakit.cc/products/e17c0027-fish-cyclic-adenosine-monophosphate-elisa-kits/">https://www.elisakit.cc/products/e17c0027-fish-cyclic-adenosine-monophosphate-elisa-kits/</a></p> <p>E17C0027 Fish Cyclic Adenosine Monophosphate ELISA kit</p> <p>Fish ELISA kit is suitable for the detection of samples from fish species. Cyclic Adenosine Monophosphate can also be called cAMP, AMP or 3',5'-cyclic adenosine monophosphate.</p> <p>camp elisa kit applies the competitive enzyme immunoassay technique utilizing an anti-cAMP antibody and an cAMP-HRP conjugate. The assay sample and buffer are incubated together with cAMP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the cAMP concentration since cAMP from samples and cAMP-HRP conjugate compete for the anti-cAMP antibody binding site. Since the number of sites is limited, as more sites are occupied by cAMP from the sample, fewer sites are left to bind cAMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cAMP concentration in each sample is interpolated from this standard curve.</p> <p>Citations of Fish cyclic amp elisa kit<br /> E17C0027 Fish cyclic amp assay kit</p> <p>Developmental toxicity by thifluzamide in zebrafish (Danio rerio): Involvement of leptin.</p> <p>As a professional drug residue test kit factory, Bluegene has high-quality meth residue test kits for sale. Welcome to contact us if needed.<a href="https://bluegenebiotech.up.seesaa.net/image/1-1-d4c76-thumbnail2.jpg" rel="nofollow"><img src="https://bluegenebiotech.up.seesaa.net/image/1-1-d4c76-thumbnail2.jpg" alt="1-1-d4c76-thumbnail2.jpg" /></a></p> 2024-04-18T18:17:21+0900 http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/wDEuMwKqZZN BluegeneBiotech http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/wDEuMwKqZZN#comments http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/wDEuMwKqZZN Fish ELISA Kits(2023年) <p><a href="https://www.elisakit.cc/products/fish-elisa-kits/">https://www.elisakit.cc/products/fish-elisa-kits/</a></p> <p>Fish are often used in environmental monitoring, embryo research, tumor research and others. Among them, zebrafish is the most commonly used. The zebrafish is a common tropical fish, which is native to South Asia. The zebrafish have a slender body with an adult length of 3~4cm, and they do not require high water quality. About 3 months after hatching, the fish reach sexual maturity, and mature fish can lay eggs every few days. The eggs are fertilized in vitro and develop in vitro. The embryo development is synchronous and fast, and the embryo body is transparent.</p> <p>Fish ELISA Kits Types<br /> Fish Cyclic Adenosine Monophosphate fish cortisol elisa kit</p> <p>Cat. No.: E17C0027</p> <p>Detection Range: 50-1000pmol/ml</p> <p>Reactivity: Fish</p> <p>Sensitivity: 1.0pmol/ml</p> <p>Fish Pyruvate Kinase A ELISA kit</p> <p>Cat. No.: E17P0666</p> <p>Detection Range: 1.0-25ng/ml</p> <p>Reactivity: Fish</p> <p>Sensitivity: 0.1ng/ml</p> <p>Fish Testosterone ELISA kit</p> <p>Cat. No.: E17T0007</p> <p>Detection Range: 250-5000pg/ml</p> <p>Reactivity: Fish</p> <p>Sensitivity: 1.0pg/ml</p> <p>As a professional drug residue test kit factory, Bluegene has high-quality meth residue test kits for sale. Welcome to contact us if needed.<a href="https://pds.exblog.jp/pds/1/202310/08/61/f0411661_12293907.jpg" rel="nofollow"><img src="https://pds.exblog.jp/pds/1/202310/08/61/f0411661_12293907.jpg" alt="f0411661_12293907.jpg" /></a></p> 2023-10-08T12:39:49+0900 http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/izMuRxYviab BluegeneBiotech http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/izMuRxYviab#comments http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/izMuRxYviab ELISA Kits(2023年) <p><a href="https://www.elisakit.cc/products/elisa-kits/">https://www.elisakit.cc/products/elisa-kits/</a></p> <p>The elisa kit company Shanghai Bluegene Biotech has focused on the research and development of ELISA kits, especially for more than a decade.</p> <p>Our ELISA Kit is a quick, convenient, and accurate research tool for the detection and quantification of targets in various samples.</p> <p>BlueGene Biotech's ELISA kits are 1.5-hour solid-phase ELISA kits, the operation is much simpler. The ELISA kits are equipped with prediluted standards to avoid the possible operational errors. Our lab performs the quality tests ahead to assure the accuracy of the standards from each batch, and then ensure a reliable standard curve. The incubation and washing steps are also easy to operate, which enables the experiment finished within 1.5 hours. We believe that fewer handling steps can reduce errors, therefore, deliver more consistent results.</p> <p>With a mature and high-tech manufacturing workshop, we are able to provide a wide range of ELISA kits but with a very short lead time and reasonable price and cost of elisa test. You may search for the products you need in the search column, or find our contact information, BlueGene Biotech team will reply to you as soon as possible.</p> <p>Why Choose BlueGene Biotech ELISA Kits?</p> <p>Simple operation (1.5-hour solid-phase ELISA): This is a very simple and easy procedure. In addition, our kits do not need to put the plate on an orbital microplate shaker during the 2 incubation steps.</p> <p>Prediluted standards (ready-to-use standards): The concentration of the standards is critical for an accurate standard curve. Therefore this kit contains serial dilutions of the standards rather than a concentrated standard solution, thereby avoiding pipetting or calculation errors. Quality tests assure that the concentration of the standards is accurate, thereby ensuring a reliable standard curve.</p> <p>Reliability: Simple incubation and washing steps enable the Elisa experiment performed within 1.5 hours. Compared to the traditional Elisa method, fewer handling steps reduce errors and deliver more consistent results. Thorough and regular tests of the system guarantee a great intra and inter-assay reliability and ensure a low coefficient of variation.</p> <p>The types of sample (both of the two types of samples can be tested using one kit)</p> <p>Sample I: serum or plasma</p> <p>Sample II: cell culture supernatants, body fluid, and tissue homogenate</p> <p>How To Use ELISA Kits?<br /> BlueGene Biotech: One-Step Competitive ELISA kit</p> <p>1) Add 100 ul Standards or Samples to the well. Add 100 ul PBS (pH 7.0-7.2) in the blank control well.</p> <p>2) Dispense 10 ul Balance Solution into 100 ul samples, mix well. (NOTE: This step should be skipped for serum or plasma samples )</p> <p>3) Add 50 ul Conjugate to each well (NOT blank control well). Cover and incubate the plate for 1 hour at 37℃.</p> <p>4) Wash the microtiter plate.</p> <p>BlueGene Biotech: One-Step Sandwich ELISA</p> <p>1) Add 50 ul Standards or Samples to the well. Add 50 uL PBS (pH 7.0-7.2) in the blank control well.</p> <p>2) Dispense 5 uL Balance Solution into 50 uL samples only, mix well. (NOTE: This step should be skipped for serum or plasma samples )</p> <p>3) Add 100 uL Conjugate to each well (NOT blank control well). Mix well. Cover and incubate the plate for 1 hour at 37℃.</p> <p>4) Wash the microtiter plate.</p> <p>Cgbio® ELISA kits-Reaearch Reagent: two-Step Sandwich ELISA</p> <p>1) Place all kits’ components at room temperature for 30mins before using.</p> <p>2) Prepare Wash Solution</p> <p>3) Prepare standards, then add 100ul diluted standards or samples to appropriate wells.</p> <p>4) Prepare Detection Antibody, then add 50ul to each wells, cover the plate and incubate for 120 mins at room temperature.</p> <p>5) Washing Step</p> <p>6) Prepare HRP-Streptavidin, then add 100ul to each well, cover the plate and incubate for 45 mins at room temperature.</p> <p>Cgbio® ELISA kits-Reaearch Reagent: three-Step Sandwich ELISA</p> <p>1) Place all kits’ components at room temperature for 30mins before using.</p> <p>2) Prepare Wash Solution (1x)</p> <p>3) Prepare Standards,then add 100ul of diluted standards or samples to appropriate wells.</p> <p>4) Cover the plate and incubate for 120 mins at room temperature.</p> <p>5) Washing Step</p> <p>6) Prepare Detection Antibody, then add 100ul to each wells, cover the plate and incubate for 60 mins at room temperature.</p> <p>7) Washing Step</p> <p>8) Prepare HRP-Streptavidin, then add 100ul to each well, cover the plate and incubate for 45 mins at room temperature.</p> <p>9) Washing Step</p> <p>10) Add 100ul TMB substrate to each well. Incubate at room temperature for 10 to 15 mins.</p> <p>For more information about drug residue test kit, please feel free to contact us!</p> <p>If you are looking for a reliable elisa kit manufacturer, don't hesitate to contact us!<br /> <a href="https://www.elisakit.cc/uploads/image/20220322/16/elisa-kit-cost.jpg" rel="nofollow"><img src="https://www.elisakit.cc/uploads/image/20220322/16/elisa-kit-cost.jpg" alt="elisa-kit-cost.jpg" /></a></p> 2023-10-08T12:39:20+0900 http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/YUDzLnK4zvV BluegeneBiotech http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/YUDzLnK4zvV#comments http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/YUDzLnK4zvV Chicken ELISA Kits(2023年) <p><a href="https://www.elisakit.cc/products/chicken-elisa-kits/">https://www.elisakit.cc/products/chicken-elisa-kits/</a></p> <p>Chickens belong to animals, chordate phylum and chordate subphylum. They are avifauna, order Galliformes, pheasant family, Genus Gallus, and subspecies of domestic chicken. The chicken embryo is the main material of the measles vaccine, influenza vaccine and rabies vaccine. The virulence of some viruses can be weakened by chicken embryo passage, which is often used in the production and detection of virus vaccines and virological research. Marek's disease is a tumor disease caused by herpesvirus, which can be prevented by vaccines. It is the first tumor that can be prevented by vaccines. It also reveals that viruses can cause tumors in the body and can be prevented by vaccines. The knee joint and cross nerve reflex of 1-7 day old chicks can evaluate the efficacy of spinal cord sedatives. And 6~14 day old chicks can be used to evaluate the effect of drugs on blood vessel function. In vitro organs of chickens are also used for drug evaluation. The reproductive function of chicken declines with age and its egg-laying can be used as an objective index for aging research. The more common indicators of our Chicken ELISA kits are Chicken Endotoxin(EDT) ELISA kit; Chicken Insulin-like Growth Factor1(IGF1) ELISA kits; chicken iga elisa kits etc.</p> <p>Chicken ELISA Kits Types<br /> Chicken Acetoacetyl CoA synthetase ELISA kit</p> <p>Cat. No.: E12A0083</p> <p>Detection Range: 2.5-50ng/ml</p> <p>Reactivity: Chicken</p> <p>Sensitivity: 0.1ng/mL</p> <p>Chicken Tumor necrosis factor related apoptosis inducing ligand ELISA kit</p> <p>Cat. No.: E12T0023</p> <p>Detection Range: 0.5-10ng/ml</p> <p>Reactivity: Chicken</p> <p>Sensitivity: 0.1ng/mL</p> <p>Chicken Vascular Endothelial Growth Factor Receptor 2 ELISA kit</p> <p>Cat. No.: E12V0004</p> <p>Detection Range: 0.5-10ng/ml</p> <p>Reactivity: Chicken</p> <p>Sensitivity: 0.1ng/mL</p> <p>As a professional drug residue test kit factory, Bluegene has high-quality research reagents for sale. Welcome to contact us if needed.<a href="https://pds.exblog.jp/pds/1/202310/08/61/f0411661_12293822.jpg" rel="nofollow"><img src="https://pds.exblog.jp/pds/1/202310/08/61/f0411661_12293822.jpg" alt="f0411661_12293822.jpg" /></a></p> 2023-10-08T12:38:47+0900 http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/FHBulzkdyoq BluegeneBiotech http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/FHBulzkdyoq#comments http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/FHBulzkdyoq Canine ELISA Kits(2023年) <p><a href="https://www.elisakit.cc/products/canine-elisa-kits/">https://www.elisakit.cc/products/canine-elisa-kits/</a></p> <p>Canine ELISA Kits<br /> We have developed thousands of ELISA kits, especially for use with canine samples. Our Canine ELISA kits have been made with highly-reliable antibodies pre-coated in the microplate to ensure a thorough reaction between antibody and antigen, and we also optimize the reaction environment for porcine samples.</p> <p>Most of our dog c3 elisa kits can be used with various samples such as serum, plasma, cell culture supernatants, body fluid and tissue homogenate samples. In order to have a better performance, we include a special balance solution for tissue-like samples which is used to make the ingredients of cell lysate, tissue homogenates, and some body fluid more similar to that of our standards and to reduce the interference of samples.</p> <p>Canine ELISA Kits Types<br /> Canine Aggrecan ELISA kit</p> <p>Cat. No.: E08A0413</p> <p>Detection Range: 2.5-50ng/ml</p> <p>Reactivity: Canine</p> <p>Sensitivity: 0.1ng/mL</p> <p>Canine Aquaporin 4 ELISA kit</p> <p>Cat. No.: E08A0467</p> <p>Detection Range: 2.5-50ng/ml</p> <p>Reactivity: Canine</p> <p>Sensitivity: 0.1ng/mL</p> <p>Canine Activin A ELISA kit</p> <p>Cat. No.: E08A0643</p> <p>Detection Range: 50-1000 pg/mL</p> <p>Reactivity: Canine</p> <p>Sensitivity: 1.0pg/mL</p> <p>As a professional drug residue test kit factory, Bluegene has high-quality meth residue test kits for sale. Welcome to contact us if needed.<a href="https://pds.exblog.jp/pds/1/202310/08/61/f0411661_12293837.jpg" rel="nofollow"><img src="https://pds.exblog.jp/pds/1/202310/08/61/f0411661_12293837.jpg" alt="f0411661_12293837.jpg" /></a></p> 2023-10-08T12:38:07+0900 http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/MQuc3Spdbna BluegeneBiotech http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/MQuc3Spdbna#comments http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/MQuc3Spdbna Distributors(2023年) <p><a href="https://www.elisakit.cc/distributors/">https://www.elisakit.cc/distributors/</a></p> <p>Australia<br /> Czech Republic<br /> Germany<br /> American<br /> France<br /> United Kingdom<br /> Netherlands<br /> Belgium<br /> Poland<br /> Italy<br /> Spain<br /> Russia<br /> Bulgaria<br /> South Korea<br /> Singapore<br /> Taiwan, China<br /> Australia</p> <p>Resolving IMAGES</p> <p>Address: 1/9 Storey Road Reservoir Vic 3073 AUSTRALIA</p> <p>Tel: 61 3 9470 4704</p> <p>E-mail: sales@resolvingimages.com-</p> <p>Web:<a href="https://www.resolvingimages.com/">https://www.resolvingimages.com/</a></p> <p>If you are looking for a reliable elisa kit distributor, don't hesitate to contact us!</p> <p>As a professional drug residue test kit factory, Bluegene has high-quality human insulin elisa kit for sale. Welcome to contact us if needed.<br /> <a href="https://www.elisakit.cc/storage/202304/jpg/ne01i0431-human-immunoglobulin-g-elisa-kit-fuben.jpg" rel="nofollow"><img src="https://www.elisakit.cc/storage/202304/jpg/ne01i0431-human-immunoglobulin-g-elisa-kit-fuben.jpg" alt="ne01i0431-human-immunoglobulin-g-elisa-kit-fuben.jpg" /></a></p> 2023-10-08T12:37:30+0900 http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/H5zsYlwfsQj BluegeneBiotech http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/H5zsYlwfsQj#comments http://pastport.jp/user/BluegeneBiotech/timeline/Bluegene%20Biotech/event/H5zsYlwfsQj