Shenzhen SEKBIO Co., Ltd

Creatine Kinase-MB (CKMB)(2024年07月01日)

SEKBIO — 2024-07-01T17:24:29+0900

https://www.sekbio.com/products/creatine-kinase-mb-ckmb/

Creatine kinase isoenzymes are an enzyme component, among which MB type is mainly present in myocardial cells. It is also a kind of cardiac marker.

CK MB protein is of great significance in clinical diagnosis. The serum creatine kinase level increases rapidly in various diseases, including muscular atrophy and myocardial infarction. The determination of creatine kinase activity is more reliable than electrocardiogram in the diagnosis of myocardial infarction. In myocardial infarction, creatine kinase increased within six h of onset, peaked at 24 h, and returned to normal within 3-4 days.

The isoenzyme CK-MB of creatine kinase has the highest specificity in diagnosis. Creatine kinase has attracted extensive attention and in-depth research because of its essential physiological function and clinical application value.

SEKBIO provides related detection raw materials: Mouse anti-human CKMB mAb and Humanized anti-human CKMB mAb.

What is Creatine Kinase MB?
The creatine kinase molecule is a dimer consisting of a brain-type subunit (B) and a muscle-type subunit (M). Normal human tissues often contain three isoenzymes, which are CK-BB (CK1), CK-MB (CK2), and CK-MM (CK3) in the order of electrophoresis. CK-MB protein mainly exists in myocardial tissue, accounting for 0-4% of CK in serum. CK and CK-MB are sensitive indicators of myocardial tissue damage, especially CK-MB is more specific. The increase of creatine kinase mb antibody mainly causes the growth of CK during myocardial injury.

CK-MB Protein
Creatine kinase is one of the essential indicators of laboratory tests. When the myocardial tissue is seriously damaged, CK-MB is released into the blood, and the serum CK-MB protein becomes a vital criterion for diagnosing acute myocardial infarction.

Increased serum ck ckmb protein is common in acute myocardial infarction, skeletal muscle injury, trauma, strenuous exercise, etc. Serum CK-MB can also be increased in malignant fever patients and some members of their families. In patients with rhabdomyosarcoma, serum CK-MB protein can be significantly increased. In addition, cardiac surgery that damages the myocardium may have a transient increase in CK-MB, which usually returns to normal within 24 hours after surgery.

Sekbio provide creatine kinase mb test, cpk mb test, cpk mb blood test, ckmb blood test, ivd antibody and etc. Want to more, contact us.

C-Reaction Protein (CRP)(2024年07月01日)

SEKBIO — 2024-07-01T17:23:52+0900

https://www.sekbio.com/products/c-reaction-protein-crp-inflammatory-marker/

C-reactive protein (CRP) refers to several proteins (acute proteins) that rise sharply in blood plasma in response to infection or tissue damage.

CRP marker can activate complement and strengthen the phagocytosis of phagocytes and play an optimizing role, thus removing pathogenic microorganisms and damaged, necrotic, and apoptotic tissue cells from the body and playing an essential protective role in the natural immune process of the body.

C-reactive protein is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells.
A CRP test measures the amount of CRP in the blood plasma samples to detect inflammation due to acute conditions or to monitor disease severity in chronic conditions.

CRP Intro
C-reactive protein (CRP) refers to some proteins (acute proteins) that rise sharply in plasma when the body is infected or tissue damaged. CRP can activate complement and strengthen the phagocytosis of phagocytes to play an opsonizing effect, thereby removing pathogenic microorganisms and damaged, necrotic, and apoptotic tissue cells that invade the body and play an important protective role in the body's natural immune process.

CRP Antibody
CRP is a non-specific inflammatory marker and directly involved in cardiovascular diseases such as inflammation and atherosclerosis and is the most potent predictor and a risk factor for cardiovascular diseases. The interaction of crp inflammatory marker with complement C1q and FcTR enables it to exhibit many biological activities, including host defense against infection, phagocytosis, and regulation of inflammatory responses. Binds to damaged cells, apoptotic cells, and nuclear antigens, making it also play an important role in autoimmune diseases.

As a professional ivd antibody factory, SEKBIO has high-quality ivd antibody for sale. Welcome to contact us if needed.

Cardiac Troponin I (cTnI)(2024年07月01日)

SEKBIO — 2024-07-01T17:23:13+0900

https://www.sekbio.com/products/cardiac-troponin-i-ctni/

Cardiac troponin I, often denoted as cTnI, is presented in cardiac muscle tissue by a single isoform with a molecular weight of 23.9 kDa. Cardiac troponin I protein consists of 209 amino acid residues. The theoretical pI of cTnI is 9.05. cTnI is more sensitive and specific in diagnosing myocardial infarction.

Phosphorylation of ctni antibody changes the conformation of the protein and modifies its interaction with other troponins and the interaction with anti-TnI antibodies. These changes alter the myofilament response to calcium and are of interest in targeting heart failure. Multiple reaction monitoring of human cTnI has revealed 14 phosphorylation sites, and the pattern of phosphorylation observed in these sites changed in response to disease.

Measuring cardiac biomarkers can be a step toward making a diagnosis for a condition. Whereas cardiac imaging often confirms a diagnosis, more straightforward and less expensive cardiac markers test can advise a physician whether more complicated or invasive procedures are warranted. In many cases, medical societies recommend doctors to make biomarker measurements an initial testing strategy, especially for patients at low risk of cardiac death.

Troponin Protein
Troponin (Tn) is a regulatory protein of muscle tissue contraction. It is located on the thin filaments of contractile proteins and plays an essential role in regulating muscle contraction and relaxation. Troponin protein contains three subtypes: fast response type and slow response type, and cardiac troponin (cTn). The first two are related to skeletal muscle. At the same time, cardiac troponin exists only in cardiomyocytes and is composed of three subunits: troponin T (cTnT), troponin I (cTnI), and troponin C (cTnC). composed of complexes. cTnT and cTnI are specific antigens of cardiomyocytes. The molecular weight of cTnI is about 23.88 KDa, which is degraded from myocardial fibers when cardiomyocytes are injured. The increase in serum cTn reflects myocardial cell damage, and its specificity and sensitivity are higher than those of the previously used myocardial enzyme spectrum.

Troponin Antibody
The presence of cTnI-specific autoantibodies in some patients' blood makes detecting the antigen very difficult.

More and more scientists at home and abroad have studied troponin autoantibodies in recent years. Different studies have shown that the detection rate of troponin autoantibodies in people without underlying cardiac diseases is about 5%. In comparison, the detection rate of troponin autoantibodies at initial diagnosis in patients with cardiac diseases (including myocarditis myocardial infarction) is between 9-16%. It reaches more than 21% in the follow-up process or patients with reinfarction. And the detection rate of cTnI autoantibodies is further improved, and the concentration of cTnI autoantibodies is significantly increased. The most probable cause of this phenomenon is the sudden myocardial infarction of the patient, which substantially increases the cTnI released into the blood circulation, stimulates the immune system to produce a robust immune memory response, and causes a significant increase in the level and affinity of cTnI autoantibodies.

As a professional hormone antibody factory, SEKBIO has high-quality hormone antibody for sale. Welcome to contact us if needed.

CA50 Tumor Marker(2024年07月01日)

SEKBIO — 2024-07-01T17:22:32+0900

https://www.sekbio.com/products/ca50-tumor-marker/

CA50 is a kind of sialic acid ester and sialic acid glycoprotein, which generally does not exist in normal tissues. When cells become malignant, glycosylation enzyme is activated, resulting in changes of glycosylation structure on the cell surface and becoming A MARKER of CA50.

The ca 50 tumor marker is a chemical that reflects the presence of a tumour.
Cancer antigen CA50 does not exist in normal adult tissues, which restricted to embryonic tissues, or in the tissue of cancer than in normal tissue, their existence or quantitative change may indicate the nature of the tumour to understand the tumour tissue, cell differentiation and cell function, to help the diagnosis, classification and prognosis judgement and treatment of tumour.

CA 50 Marker Intro
CA50 is a chemical class that reflects the presence of tumors. They do not exist in normal adult tissues but only in embryonic tissues, or the content in tumor tissues dramatically exceeds that in normal tissues. Their presence or quantitative changes can indicate the nature of tumors to understand tumor histogenesis, cell differentiation, and cell function to help tumor diagnosis, classification, prognosis, and treatment guidance.
Cancer Antigen 50
CA50 antibody is a sialic acid ester and sialic acid glycoprotein. When cells become malignant, glycosylase is activated, resulting in the change of cell surface glycosyl structure and becoming the marker of CA50. CA50 in normal blood is less than 20 μg/L, and it can elevate in many malignant tumor patients, such as 66.6% of lung cancer, 88.2% of liver cancer, 68.9% of gastric cancer, 88.5% of ovarian or cervical cancer, and 94.4% of pancreatic or bile duct cancer. Others, such as rectal cancer, bladder cancer, etc., are more than 70% elevated.

As a professional hormone antibody factory, SEKBIO has high-quality hormone antibody for sale. Welcome to contact us if needed.

CA242 Tumor Marker(2024年07月01日)

SEKBIO — 2024-07-01T17:20:37+0900

https://www.sekbio.com/products/ca242-tumor-marker/

CA242, or Cancer Antigen 242, is a notable glycoprotein biomarker within the realm of oncology and the biomedical industry. It is classified as a mucin, a type of glycoprotein characterized by extensive glycosylation and an often elongated structure. CA242 is composed of a unique amino acid sequence, primarily consisting of tandem repeats rich in proline, threonine, and serine residues. These repeats contribute to its mucinous and glycosylated nature, facilitating its distinctive role in various types of cancer.

The amino acid sequence of CA242, which varies slightly between individuals, comprises a central core protein with extensive glycosylation sites. The glycosylation of CA242 involves the addition of carbohydrate molecules, particularly O-linked glycans, enhancing its stability and modulating its functions. The specific amino acid sequence and glycosylation pattern are critical factors in the structural integrity and functionality of CA242, making it an important target for detection and analysis in cancer diagnostics.

The detection and accurate quantification of CA242 in biological samples are crucial for diagnosing and monitoring the progression of gastrointestinal cancers, where CA242 is often overexpressed. Understanding the intricacies of CA242's amino acid sequence and structure is fundamental in advancing the design and optimization of detection methods, ultimately contributing to enhanced cancer diagnostics and treatment strategies within the biomedical industry.

CA242 carbohydrate antigen is a kind of saliva acidification that can be colon cancer cell lines by a series of monoclonal antibody hybridoma technology is one of CA242 tumor marker recognition.
Many organs of malignant tumor mucin types of a glycoprotein found, called Cang, which cannot react with LewisA antigens and cannot react with saliva acidification galactose glucoside.

CA242 tumor marker is almost always expressed together with CA50, but different monoclonal antibodies recognize the two. Clinically, the CA242 tumor marker has been used in the diagnosis of gastrointestinal malignancies, especially pancreatic and colorectal cancers.

Compared with CA19-9 tumour marker and CA50, the new generation CA242 tumor marker has higher sensitivity and specificity in pancreatic, gallbladder, and gastrointestinal cancers.

CA242 Tumor Marker Intro
Cancer antigen 242, a sialylated glycosphingolipid antigen, is a pancreatic and colon cancer marker. The increase is seen in 68% ~ 79% of pancreatic cancer, 55% ~ 85% of colon cancer, 44% of gastric cancer, and 5% ~ 33% of non-malignant tumors. Also, the positive rates of ovarian, uterine, and lung cancer were higher than CA50.

CA242 Blood Test
Cancer antigen 242 is a marker for the digestive system, especially colon, rectal and pancreatic cancer. It is superior to CA19-9 in diagnosing pancreatic cancer, with a sensitivity of 66% to 100%. It is also helpful for diagnosing liver cancer, gastric cancer, and esophageal cancer. However, ca242 blood test is not suitable for diagnosing squamous cell carcinoma.

Reference value: -12 units/ml (u/ml) (IRMA method).

As a professional tumor marker factory, SEKBIO has high-quality tumor marker for sale. Welcome to contact us if needed.

CA19-9(2024年07月01日)

SEKBIO — 2024-07-01T17:19:45+0900

https://www.sekbio.com/products/ca19-9-tumor-marker/

CA19-9 Tumor Marker
Cancer antigen 19-9 (CA199 Marker )refers to a tumor marker associated with pancreatic, gallbladder, colon, and stomach cancers and is also known as an associated gastrointestinal antigen.
Cancer antigen 19-9 has high sensitivity and specificity for pancreatic cancer. Detection of ca199 tumor marker is helpful for differential diagnosis, disease monitoring, and diagnosis of pancreatic cancer metastasis.

SEKBIO is now offering Mouse anti-human CA19-9 mAb and Humanized anti-human CA19-9 mAb. These antibodies are suitable for use in diagnostics, including ELISA, lateral flow, CLIA, etc. And our products are highly consistent with Roche kits and similar antibodies of CanAg.

Tumor Marker CA19-9 Antigen
Cancer antigen 199(CA19-9 Tumor Marker ) refers to a tumor marker associated with pancreatic cancer, gallbladder cancer, colon cancer, and gastric cancer, also known as a gastrointestinal tract-associated antigen. tumor marker ca199 has high sensitivity and specificity for pancreatic cancer, and its positive rate is between 85% and 95%, and it decreases with the improvement of the condition after surgery.

CA199 Test
The determination of CA19-9 is helpful for the differential diagnosis and condition monitoring of pancreatic cancer. When CA19-9 is less than 1000 U/ml, it has specific surgical significance. After tumor resection, the concentration of CA19-9 will decrease. If it rises again, it can indicate recurrence. The diagnosis of pancreatic cancer metastasis also has a high positive rate. Peripheral metastasis is almost always present when the serum CA19-9 level is higher than 10000 U/ml.

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Inflammatory Marker(2024年07月01日)

SEKBIO — 2024-07-01T17:18:49+0900

https://www.sekbio.com/products/blood-test-inflammatory-marker/

Inflammatory cytokines refer to various cytokines involved in the inflammatory response. And the inflammatory markers can identify the severity and fatality of the disease.
Procalcitonin (PCT), serum ferritin, C-reactive protein (CRP), and interleukin-6 (IL-6) are significantly associated with the high risks of the development of severe disease. And Serum amyloid A (SAA) can serve as a biomarker for monitoring disease progression.
SEKBIO is now offering antibodies, antigens, and CLIA bulk reagents for these test items. These products' performance has been evaluated based on the CLIA method, head-to-head comparison with Roche ECLIA product, and the consistency shows excellent consistency.

Inflammatory Marker Intro
Inflammation marker refer to the indicators on which inflammatory diseases are judged in clinical diagnosis. When bacteria (or viruses) infect biological organisms, the body often has an inflammatory response, manifested as fever, increased white blood cell count, accelerated erythrocyte sedimentation rate (erythrocyte sedimentation rate), and increased various acute-phase proteins.

Inflammatory Markers Blood Test
Whether inflammation occurs and the severity of inflammation can be judged by white blood cell count (WBC), erythrocyte sedimentation rate, and acute-phase protein concentration detection. Generally, WBC, erythrocyte sedimentation rate, and acute-phase protein are inflammatory biomarker.
New inflammatory markers include procalcitonin, C-reactive protein, serum amyloid A, interleukin 6, etc. The content of these markers in the body is not affected by antibiotics, immunosuppressants, and hormones and can be used as symptoms of inflammation reliable indicator.

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Procalcitonin PCT(2024年04月19日)

SEKBIO — 2024-04-19T16:37:31+0900

https://www.sekbio.com/procalcitonin-pct.html

Procalcitonin PCT
Procalcitonin metabolism is a peptide precursor of the hormone calcitonin, the latter being involved with calcium homeostasis. It arises once preprocalcitonin is cleaved by endopeptidase.

Details
Current targeting conventional markers(C-reactive protein, white blood cell, tumor necrosis factor-α, interleukins, etc.) are non-specific for diagnosing sepsis. Procalcitonin (PCT), a member of the calcitonin superfamily, could be a critical tool for diagnosing sepsis. But to distinguish between bacterial versus viral infections, procalcitonin alone may not be effective. Rapid elevation in the concentration of procalcitonin and other newly emerging biomarkers during disease and its correlation with severity of illness makes it an ideal biomarker for bacterial infection. Besides this, the pct protein levels can be used to monitor response to antimicrobial therapy, diagnose secondary inflammations, diagnose renal involvement in pediatric urinary tract infection, etc.

The PCT antibody pair’s sensitivity is evaluated based on the lateral flow fluorescence immunoassay method.

Sekbio is your reliable contract developer and manufacturer of immunoassay and IVD antibody.

What is Human Epididymis Protein 4 (HE4)?(2024年04月19日)

SEKBIO — 2024-04-19T16:36:58+0900

https://www.sekbio.com/what-is-human-epididymis-protein-4-he4.html

1. What is human epididymis protein 4 (HE4)?

It is a new tumor marker for ovarian cancer, which ranks third among the three major gynecologic malignancies in terms of incidence. It is not easy to be detected because of its early symptoms. As the time of cancer increases, the 5-year survival rate of patients decreases from 70%-90% to 20%, so early diagnosis is crucial to the cure rate of ovarian cancer.

HE4, called human epididymis protein 4, is a sensitive indicator for detecting ovarian cancer and is one of the antigen markers. It is another highly recognized tumor marker for epithelial ovarian cancer after CA125. Human epididymis protein 4 (HE4) is not expressed in the normal ovarian surface epithelium, while it is significantly highly expressed in serous ovarian cancer and endometrioid ovarian cancer.

Compared with the glycoantigen CA125, which is less disturbed by external factors, the cancer antigen 125 marker is generally disturbed by many factors such as inflammation, endometriosis, and pregnancy, which can cause elevation, whereas human epididymis protein 4 (HE4) is generally not affected by these factors. The combined test of he4 human epididymis protein 4 and CA125 is more meaningful for detecting ovarian cancer.

Human epididymis protein 4 (HE4) combined with CA125 has high clinical value in the early diagnosis of epithelial ovarian cancer, disease monitoring, postoperative recurrence monitoring, as well as in the differential diagnosis of benign tumors. HE4 protein also has some sensitivity in the diagnosis of endometrial cancer. It is also seen in other tumors, such as the lung, breast, and pancreas. It is also seen in the increase of human epididymis protein 4. It is also increased in some gynecological inflammatory diseases, ovarian cysts, and other benign tumors.

2. Clinical significance of human epididymis protein 4 (HE4)
HE4 tumour marker is expressed at high levels in ovarian cancer tissues and serum, as well as in endometrial, lung, colon, and breast cancers. Increased percentage of detectable serum human epididymis protein 4 (HE4) levels have been used as an aid to monitor the recurrence and progression of disease in patients with invasive epithelial ovarian cancer.

The association of human epididymis protein 4 (HE4) levels with clinical responsiveness to treatment or recurrence status in patients with ovarian cancer examined by CT imaging suggests that human epididymis protein 4 (HE4) may be an important indicator of ovarian cancer recurrence.

Elevated levels of HE4 may also be seen in other malignant disorders such as breast cancer, endometrial cancer, and, rarely, benign disorders.

Learn more about tumor markers list such as ca199 marker. Contact us for more information.

Sekbio has specialised in immunoassay for years. Our team possesses extensive expertise in the development and production of immunoassays and antibodies. (2) GMP compliant facility and ISO13485 quality system to ensure the product quality. (3) Sophisticated immunoassay technology platforms have been established, including CLIA, ELISA, LFA

If you want to know more about cardiac markers blood test, please visit our website.

The Bottleneck in the R&D of IVD Raw Materials Needs to Be Broken Through and the Competitiveness of the Industry Needs to Be Improved(2024年04月19日)

SEKBIO — 2024-04-19T16:34:24+0900

https://www.sekbio.com/the-bottleneck-in-the-rd-of-ivd-raw-materials-needs-to-be-broken-through-and-the-competitiveness-of-the-industry-needs-to-be-improved.html

The Bottleneck in the R&D of IVD Raw Materials Needs to Be Broken Through and the Competitiveness of the Industry Needs to Be Improved
1. Development of China's IVD raw material industry
As China's ivd raw materials industry started late, the market is highly fragmented, and brand trust is low, there are still many difficulties in realizing domestic substitution. Compared with overseas IVD raw material enterprises, China's IVD raw material brands need to advance in R&D capabilities and production technology.

IVD involves a variety of diagnostic methodologies, and each procedure consists of various diagnostic items, requiring a wide variety of reagents and instruments, and the technical system is quite different. Therefore, IVD raw material enterprises need to build a complete technical approach and a more systematic and rigorous quality control system, r&d ability, quality control, and other procedures to enhance their core competitiveness.

2. Global market development of IVD raw materials
At present, the global market of IVD raw materials as a whole presents the characteristics of many participants, small individual scale, and insufficient industry concentration. Even the leading companies have a relatively low global market share. It is challenging for a single company to include comprehensive product lines. The IVD raw material industry involves many fields and technology platforms. Therefore, IVD raw material companies can establish a complete and rich product matrix through endogenous development and M&A expansion, covering a variety of application scenarios and better meeting the diverse needs of downstream customers.

In addition, the ivd raw materials market has high brand barriers. To ensure product quality, IVD manufacturers usually spend a lot of time and money in the research and development stage to verify the reliability of raw materials. Therefore, many production enterprises choose core raw material manufacturers with long operating years and high product popularity, and customer stickiness continues to increase. Thus, insisting on good word-of-mouth marketing and customer service, and gradually building a brand image with market influence, is also a way for enterprises to achieve breakthrough development.

The advantages of branding are becoming increasingly prominent. In fact, with the continuous investment of Chinese enterprises in technology and research and development, some domestic brands have broken through key technical barriers, and their product performance can compete with global IVD brands. Moreover, Chinese IVD raw material suppliers have more advantages in logistics supply and technical services, such as more controllable production cycle and transportation procedures, shorter supply cycle, and lower communication costs than multinational companies and demanders.

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We can provide high quality tumor antigens for sale, if you have needs, please contact us.

Soluble Fms-like tyrosine kinase-1 (sFlt-1)(2024年04月19日)

SEKBIO — 2024-04-19T16:33:28+0900

https://www.sekbio.com/soluble-fms-like-tyrosine-kinase-1-sflt-1.html

Soluble fms like tyrosine kinase-1 is a tyrosine kinase protein with antiangiogenic properties. A non-membrane-associated splice variant of VEGF receptor 1, sFlt-1 binds the angiogenic factors VEGF and PlGF, reducing blood vessel growth by reducing free VEGF and PlGF concentrations. Sflt 1 antibody/PLGF ratio is valuable for assessing the severity of preeclampsia and diagnosing early-onset preeclampsia and can be used to predict neonatal birth weight.

Since its establishment, SEKBIO has established long-term and stable partnerships with IVD customers from numerous countries in Europe, North America, Asia, and beyond. we are committed to providing excellent customer service and high-quality products. We understand the importance of time and quality in biological research and applications, and therefore we guarantee to deliver satisfactory results with the fastest speed and optimal quality.

If you want to know more kinds of ivd raw materials, please visit our website.

Parathyroid Hormone (PTH)(2024年04月19日)

SEKBIO — 2024-04-19T16:32:48+0900

https://www.sekbio.com/parathyroid-hormone-pth.html

Parathyroid Hormone Recombinant Monoclonal Antibody
Parathyroid hormone, also called parathormone or parathyrin, is a hormone secreted by the glands that regulate the serum calcium concentration through its effects on bone, kidney, and intestine. PTH and vitamin D antibody (25-OH Vitamin D) both belong to bone marker products.

Sekbio provide the ready-to-use immunoassays and IVD antibodies, while ODM service is also available to tailor your need.

Ovarian Cancer Detection Index: Human Epididymis Protein 4 (HE4)(2024年04月19日)

SEKBIO — 2024-04-19T16:32:07+0900

https://www.sekbio.com/ovarian-cancer-detection-index-human-epididymis-protein-4-he4.html

Sekbio Ovarian Cancer Detection Index: Human Epididymis Protein 4 (HE4)
As the most common malignant tumor of female reproductive organs and the first cause of death from gynecological malignancies in China, ovarian cancer has seen a 30% increase in incidence and an 18% increase in mortality over the past 10 years. Because early symptoms are not obvious and early tumors are difficult to detect during pelvic examinations, 70% to 75% of ovarian cancer patients are already in advanced stages when diagnosed, which is why ovarian cancer is called the "silent killer".

Human epididymis protein 4 ovarian cancer (HE4) is a new tumor marker that can help in early diagnosis, differential diagnosis, treatment monitoring, and prognosis assessment of ovarian cancer. It is a real benefit for women's health.

1. What is human epididymis protein 4 (HE4)?
HE 4 human epididymis protein 4 belongs to the whey acidic 4-disulfide center (WFDC) protein family, which has suspected trypsin inhibitor properties. Other proteins in this family include SLPI, Elafin, and PS20 (WFDC1). The HE4 gene encodes a segment of 13 kD in length, although, at the time of its mature glycosylation formation, this protein is roughly 20 kD to 25 kD and contains a single chain with two WFDC structural domains.

HE4 was first identified in the epithelium of the distal epididymis and was initially thought to be a protease inhibitor associated with sperm maturation. Immunohistochemical and gene microarray studies confirmed that it is barely expressed in normal human ovarian tissues; in malignant tissues, it is commonly upregulated in ovarian cancer and is one of the most commonly upregulated expressed genes in ovarian cancer; and is not expressed or is lowly expressed in most non-ovarian cancers. In contrast, RT-PCR studies revealed that HE4 was highly expressed in ovarian cancer cell lines, and cell cultures contained a secreted form of HE4.

In a comparative study of ovarian cancer patients with healthy states, Hellstrom et al. found that HE4 was up to 95% specific and 67% sensitive for detecting ovarian cancer. The specificity was significantly higher than that of the cancer antigen 125 marker alone. Combined with CA125, the test was more accurate in predicting ovarian cancer, with a sensitivity and specificity of 76% and 95%, respectively.

2. Application of human epithelial protein 4 (HE4) test in ovarian cancer patients
Ovarian cancer is one of the common malignant tumors threatening women's health. 70% of the patients are already in the advanced stage when diagnosed, and the five-year survival rate of advanced-stage patients is only 20% to 30% despite tumor cytoreductive surgery and chemotherapy. At present, the diagnosis of ovarian cancer mainly relies on clinical symptoms, pelvic examination, vaginal ultrasound, CT/MRI, and serum tumor markers.

The commonly used antigen markers include CA125, HCG, CEA, TPA, etc. Especially CA125 is the most widely used and plays an important role in clinical diagnosis, chemotherapy monitoring, and follow-up; however, due to its low specificity, it has little significance in the early screening of ovarian cancer. Therefore, there is a lack of effective means for early diagnosis of ovarian cancer in clinical practice. And the discovery of HE4 assisted in solving this problem to a great extent.

3. Application of human epithelial protein 4 (HE4) test

(1) The combined test with CA125 is used for risk prediction index (PI) calculation of malignant ovarian cancer to assess the risk of epithelial cell type ovarian cancer in premenopausal and postmenopausal women with pelvic tumors (predicted probability ROMA value).

(2) Disease status monitoring of patients diagnosed with ovarian cancer.
Several studies have demonstrated that changes in HE4 tumour marker concentration levels in ovarian cancer patients are positively correlated with changes in disease progression. Therefore, the progression of ovarian cancer can be assessed by continuous measurement of serum HE4 concentration levels.

(3) Note on limitations of application:
HE4 test levels may be at normal values in some patients with diagnosed ovarian cancer and may be seen in mucin-type ovarian cancer or germ cell-type ovarian cancer, both of which rarely express human epithelial protein 4 (HE4);

The concentration level of HE4 is not absolute evidence for the presence or absence of malignancy;

The rules of risk assessment for malignant ovarian cancer do not apply to patients undergoing chemotherapy and those younger than 18 years of age.

Myo - The earliest abnormally increased cardiac protein marker after myocardial injury(2024年04月19日)

SEKBIO — 2024-04-19T16:31:18+0900

https://www.sekbio.com/myo---the-earliest-abnormally-increased-cardiac-pr.html

Myo - The earliest abnormally increased cardiac protein marker after myocardial injury
Myoglobin (Myo) is a binding protein composed of a peptide chain and a heme prosthetic group. It is mainly distributed in cardiac and skeletal muscle tissues and has the function of transporting and storing oxygen in muscle cells. When the myocardium is damaged, it diffuses out of the myocardium and enters the blood circulation.

Myo protein is a small molecular substance, which is the earliest abnormally increased cardiac marker after myocardial injury. In acute myocardial infarction (AMI), the serum myoglobin concentration deviates from the normal value within 2 to 3 hours in the initial stage of chest pain, reaches the highest value in 6 to 9 hours, and returns to normal in about 24 hours.

Therefore, within 1.5 to 6 hours after the occurrence of AMI, the dynamic detection of serum myoglobin level can early diagnose whether AMI occurs. If the second detection value is significantly higher than the first detection value, it has an extremely high positive predictive value; if there is no difference between the two detected values of dynamic detection, it has a 100% negative predictive value, excluding the possibility of AMI.

Blood myoglobin concentrations are useful for monitoring the diagnosis and treatment of AMI. And it can be used as an indicator of coronary re-dredging in thrombolytic therapy. Myoglobin concentration reaches the highest after 30 minutes to 2 hours of coronary dredging.

Myo antibody has high sensitivity in the early detection of myocardial injury, but poor specificity. It should be noted that myoglobin may be elevated in severe shock, severe extensive trauma, end-stage renal insufficiency, myocarditis, acute infection, myositis, or myopathy. Therefore, attention should be paid to the differential diagnosis with AMI, and the nature of chest pain cannot be determined based on the Myo single test results.

Today, clinical diagnosis of AMI usually requires combined detection of cTnI antibody CKMB, and Myo. The sensitivity of CKMB protein is lower than Myo in the early diagnosis of AMI, but the specificity is higher. cTnI has myocardial specificity and is the most reliable marker for the detection of myocardial infarction, but it begins to increase 3-8 hours after chest pain occurs. If cTnI is detected alone, it may not be able to diagnose early AMI patients. Therefore, the dynamic combo test of Myo, cTnI, and CKMB within 6 hours of chest pain is helpful to timely and accurately distinguish AMI patients, thereby creating conditions for the timely rescue of patients.

Sekbio Incorporated is a prestigious biotech developer and manufacturer specialised in immunoassay, based in Shenzhen-the thriving innovation hub of China.

Clinical Significance of Troponin Ic(2024年04月19日)

SEKBIO — 2024-04-19T16:29:32+0900

https://www.sekbio.com/clinical-significance-of-troponin-ic.html

Troponin ic measurement is mainly used for the laboratory diagnosis of acute myocardial infarction, the prognosis of unstable angina pectoris, and the treatment monitoring of thrombolytic drugs after AMI. At the same time, it has certain diagnostic value for other diseases with myocardial cell damage.

1. The diagnosis of myocardial injury by ctni troponin
Among the many clinical biochemical indicators for the diagnosis of acute myocardial infarction (AMI), CK MB protein was once considered the "gold standard", and has been widely used for many years.

With the in-depth study of cardiac ctni troponin (cTn), the status of CK-MB has been seriously challenged both in terms of myocardial specificity and diagnostic sensitivity. The cTn is considered to be the best definite marker at present, and is gradually replacing CK-MB as the "gold standard" for the diagnosis of AMI. Cardiomyocyte damage is bound to occur in patients with various coronary artery diseases.

The clinical manifestations of some patients may not fully meet the WHO diagnostic criteria for AMI (unstable angina is one of them), but they are accompanied by elevated myocardial injury markers (such as cTnT, etc.), resulting in the leakage of cellular components into the peripheral blood circulation. This makes it possible to detect markers of cardiomyocyte damage.

2. Troponin ic as an indicator of thrombolytic therapy after AMI
Intravenous injection of thrombolytic drugs is a commonly used treatment method for AMI in recent years, and judging whether reperfusion occurs after treatment has also become one of the most concerned issues for clinicians.

There are often bimodal changes in cTnT during reperfusion: on the 1st day, after the infarction is opened, the blood flow enters the lesion site, and the free cTnT is flushed into the blood, and the first peak appears; on the 4th day, the second smaller peak can be observed (mainly from cTnT in the cTn complex). The ratio of these two peaks is helpful for judging whether reperfusion occurs: if the first peak is greater than the second peak, that is, the ratio is greater than 1.0, it often means that reperfusion occurs.

A comparative study of the early kinetics of CK-MB, myoglobin (Mgb), cTnT and cTnI, indicators of early coronary reperfusion, after successful thrombolysis showed that the early kinetics of the four markers released after thrombolysis were basically similar, but the mean index of coronary reperfusion at 90 min was significantly greater for cTnT and cTnI antibody than for CK-MB and Mgb.

3. Troponin ic in the diagnosis of perioperative myocardial infarction
The diagnosis of myocardial infarction after coronary artery bypass grafting plays an important role in cardiac surgery. The cTn is a sensitive and specific marker of perioperative myocardial infarction, which can identify minor perioperative myocardial injury that does not meet the criteria of conventional perioperative myocardial infarction.

4. Troponin ic for the diagnosis of myocarditis
Compared with CK activity, cTnT has higher detection sensitivity in myocarditis due to its relatively high serum detection value and longer rise time, and serum cTnT can be used as a diagnostic marker for acute myocarditis.

5. The relationship between ctni troponin and renal failure
Ischemic heart disease is one of the leading causes of morbidity and mortality in patients with end-stage renal disease, accounting for approximately 40% of total mortality; approximately 25% of these ischemic heart diseases develop AMI. Therefore, in the clinical treatment of patients with advanced renal disease, the diagnosis of cardiovascular complications has become a crucial issue. There are differences in the detection values of cTnT and cTnI in the serum of patients with advanced kidney disease.

6. Differential diagnosis of skeletal muscle injury by ctni troponin
Cardiac and skeletal muscle cells are closely related. During the embryonic period, the two muscles shared many similarities in gene expression, but the expression was different during terminal differentiation. Therefore, the specificity of myocardial injury marker detection in patients with skeletal muscle injury is a matter of concern.

As the second generation cTnT assay has ruled out the cross-reactivity of the assay, cTn can be used as a better marker for the diagnosis of myocardial injury in patients with skeletal muscle injury.

7. Diagnosis of myocardial injury in hypothyroid patients by ctni troponin
Hypothyroidism leads to elevated cholesterol, predisposing patients to coronary artery disease and AMI. At the same time, patients with hypothyroidism often have symptoms of skeletal muscle damage such as cramps and myalgia. Therefore, the serum CK and CK-MB of this kind of patients all increased to varying degrees. At this time, cTn is a better marker for the diagnosis of myocardial injury in hypothyroid patients.

8. Observation of the drug effects by ctni troponin
The cTn is also used to observe the relationship between the pharmacological effects of certain drugs and the heart, to understand whether to improve or exacerbate myocardial ischemia.

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25-OH Vitamin D(2024年02月07日)

SEKBIO — 2024-02-26T15:48:47+0900

https://www.sekbio.com/products/vitamin-d-antibody/

Vitamin D is converted to 25 hydroxy d vitamin (25ohd vitamin d) by hydroxylation in the liver and the active 1, 25-dihydroxy vitamin D in the kidneys.
25 hydroxyvitamin D is the main form of vitamin D in the body.
The level of serum 25 oh d vitamin d can reflect the level of vitamin D storage and correlate with the clinical symptoms of vitamin D deficiency
Similar to other steroid hormones, 1, 25 oh vitamin d3 regulates tissue cell differentiation and related gene expression, mainly regulating blood calcium level and affecting cell differentiation.
25-OH-VD has a high content in blood and a long half-life.
It is stable and is not directly affected by diet and lifestyle. Therefore, clinical measurement of 25 oh vitamin d can reflect the proper level of vitamin D in vivo.
The performance date of 25-OH-VD Rapid Quantitative Test Kit (FIA)
ManufacturerSEKBIO
Parameter25-OH-VD Rapid Quantitative Test Kit (FIA)
Linearity5~100ng/mL
Detection limit5ng/mL
Benchmark productRoche

Method Comparison
SampleRocheSekbioSampleRocheSekbio
178.0290.713165.7366.38
291.6178.973230.4234.37
355.1745.843320.9623.14
479.1389.793497.9784.54
525.6224.293559.4160.24
626.7222.68367.146.97
76.436.383741.4735.85
820.4719.583818.8318.69
939.4243.853919.3417.11
1063.0066.964020.0818.41
1116.1716.504156.4857.70
1215.0216.244256.9059.08
1334.9131.644314.0413.45
149.119.574480.3488.07
1588.3794.534578.1488.27
1694.9191.894620.0819.09
1788.6292.174732.5136.18
1869.3666.034876.2169.40
1955.0461.924915.3015.31
207.787.25505.775.69
2169.5469.225118.2517.30
2265.4670.735284.6097.40
2348.0744.215314.8716.25
2424.3126.415428.8724.48
257.406.415568.3572.81
2663.9757.945642.6042.17
2742.0343.315715.0317.40
2877.1572.945817.8418.93
2913.3811.325911.069.91
3051.6559.586084.6378.25

Linear
CalConcentrationResultAVERAGE
S14.764.804.86
5.19
4.59
S214.7815.7514.98
13.18
16.01
S331.6734.7233.01
33.23
31.07
S460.8462.6860.91
58.22
61.83
S581.5678.1076.20
77.02
73.48
S6103.58109.51113.18
123.39
106.63

Accuracy
CalconcentrationResultBias
A114.7815.474.6%
15.585.4%
16.229.7%
A231.6732.703.3%
29.35-7.3%
29.57-6.6%
A360.8457.26-5.9%
63.464.3%
63.464.3%

Precision
CalConcentrationCalConcentrationCalConcentration
R114.19R235.21R359.28
14.0427.3761.50
16.5030.9354.36
15.7132.5861.36
15.2230.9852.39
16.9028.4568.70
13.1036.2763.53
15.5731.4154.62
14.8127.0662.28
13.5434.8857.39
AVE14.96AVE31.51AVE59.54
SD1.25SD3.26SD4.96
CV8.4%CV10.4%CV8.3%

Vitamin D Products
AntibodyApplication
Sheep Anti-human 25-OH Vitamin D mAbFor immunodiagnostic: ELISA, LFA, CLIA
Mouse Anti-human 25-OH Vitamin D mAb
Humanized Anti-human 25-OH Vitamin D mAb

VD product Introduction
Optimal Vitamin D status is determined by measuring the metabolite 25-OH vitamin D in the bloodstream. This form of vitamin D serves as the primary storage compound in the human body, significantly outweighing the biologically inactive circulating 1,25 (OH)2 vitamin D by approximately 1000-fold.
The circulating half-life of 25-OH vitamin D is approximately 2-3 weeks. It's worth noting that over 95% of measurable 25 0h vitamin d in the serum consists of 25-OH vitamin D3, while 25ohd2 becomes detectable only in individuals who supplement with vitamin D2.
Vitamin D-binding protein
The human GC is approximately 58 kDa in size and consists of 458 amino acids. Its genetic code is comprised of 1690 nucleotides located on chromosome 4 (4q11–q13). A notable feature of GC is its primary structure, which includes 28 cysteine residues forming multiple disulfide bonds. The protein is divided into three domains: domain 1 comprising 10 alpha helices, domain 2 consisting of 9 helices, and domain 3 with 4 helices.
Interestingly, the metabolism of these domains appears to differ, particularly in their interaction with vitamin D. Research indicates that the vitamin D binding protein, a component of GC, may exhibit a higher affinity for 25(OH)D3 (the metabolite of vitamin D3) compared to 25(OH)D2 (the metabolite of vitamin D2). This finding was observed in a specific study.
Six point calibration test results
Conc.ng/mlRLUAVERCVS/N
0.001988864198995119894080.04%1.00
30.001701638171760317096210.66%1.16
75.001199394120140212003980.12%1.66
150.006809936772046790990.39%2.93
300.003128203186603157401.31%6.30
600.001479141460621469880.89%13.53

Precision

Conc.ng/mlDiluent RatioRLUAVECVCalculated Conc.
30.000.017013781678029168282616874110.7%32.52
144.000.27058447009507228567098831.6%143.40
258.000.43764813829963869173821311.4%254.55
372.000.62491002480732553302508341.6%366.56
486.000.81893571912511882251896110.8%470.47
6001.01478711467791504221483571.3%594.27
25-OH Vitamin D Intro
25-hydroxyvitamin D is the main form of vitamin D in the body. Vitamin D is a steroid derivative, is a fat-soluble vitamin, and is a cyclopentane poly hydro phenanthrene compound. Vitamin D is mainly synthesized by human skin after being irradiated by ultraviolet rays, and a small part is ingested from food or supplements. Vitamin D affects calcium and phosphorus metabolism and has a wide range of physiological effects. It is an indispensable substance for maintaining human health, cell growth, and development and is closely related to various diseases. There are two forms of vitamin D in the human body, vitamin D3 (cholecalciferol) and vitamin D2 (ergocalciferol), which are converted to 25-hydroxyvitamin D (25-OH-VD) by hydroxylation in the liver, which is then converted to active 1,25-dihydroxy vitamin D in the kidneys. The serum 25-OH-VD can reflect human vitamin D storage level, which is related to the clinical symptoms of vitamin D deficiency.
Vitamin D Antibody
Vitamin D is an important hormone that regulates calcium and phosphorus metabolism in the body. The skin mainly synthesizes it under sunlight conditions, and a small amount can obtain through diet. The active form of vitamin D is especially 1,25 dihydroxy vitamin d3 antibody (1,25(OH) 2 D 3 ), which exerts physiological effects by binding to the vitamin D receptor (VDR). 25-hydroxyvitamin D (25(OH)D) is the body's primary storage form of vitamin D. 1,25(OH)D3 induces the differentiation of monocytes into macrophages and reduces the release of inflammatory cells and chemokines.
For more information about vitamin antibody and ivd raw materials, , please feel free to contact us!
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Tumor Marker(2024年02月06日)

SEKBIO — 2024-02-26T15:47:57+0900

https://www.sekbio.com/products/tumor-marker/

A antigen markers is a chemical substance that reflects the presence of a Tumor. It is present in malignant tumour cells, not in normal adult tissues, and substances abnormally produced by malignant tumour cells or by the host's response to tumour stimulation created it.
Their presence or quantitative changes can indicate the nature of the tumour to understand the histogenesis, cell differentiation, and cell function of the tumour.

Detection of scc antigen tumor marker has the following functions:
Early detection of tumour;
Tumour census and screening;
Diagnosis, differential diagnosis and staging of tumours;
Monitoring the efficacy of surgery, chemotherapy and radiotherapy for tumour patients;
Indicators of tumour recurrence;
Prognosis of tumour;

Looking for the primary foci of metastases of unknown origin.
Tumor Marker Products
CA50
AntibodyApplication
Mouse anti-human CA50 mAbFor immunodiagnostic: ELISA, LFA, CLIA
Humanized anti-human CA50 mAb

CA125
AntibodyApplication
Mouse anti-human CA125 mAbFor immunodiagnostic: ELISA, LFA, CLIA
Humanized anti-human CA125 mAb

CA242
AntibodyApplication
Mouse anti-human CA242 mAbFor immunodiagnostic: ELISA, LFA, CLIA
Humanized anti-human CA242 mAb

CA15-3
AntibodyApplication
Mouse anti-human CA15-3 mAbFor immunodiagnostic: ELISA, LFA, CLIA
Humanized anti-human CA15-3 mAb

CA19-9
AntibodyApplication
Mouse anti-human CA19-9 mAbFor immunodiagnostic: ELISA, LFA, CLIA
Humanized anti-human CA19-9 mAb

CA72-4
AntibodyApplication
Mouse anti-human CA72-4 mAbFor immunodiagnostic: ELISA, LFA, CLIA
Humanized anti-human CA72-4 mAb

Pepsinogens I (PGI)

AntibodyApplication
Anti-human PGI monoclonal antibodyFor immunodiagnostic: ELISA, LFA, CLIA

AntigenApplication
PGI antigenFor immunodiagnostic: ELISA, LFA, CLIA

Human Epididymis 4 (HE4)

NameApplication
Recombinant human HE4 protein (Prokaryotic HE4 full-length protein)For immunodiagnostic: ELISA, LFA, CLIA

Prostate-Specific Antigen (PSA)

AntibodyApplication
Mouse anti-human PSA mAbFor immunodiagnostic: ELISA, LFA, CLIA
Humanized anti-human PSA mAb
Mouse anti-human free PSA mAb

AntigenApplication
Prostate-Specific Antigen (Eukaryotic expression)For immunodiagnostic: ELISA, LFA, CLIA

Squamous Cell Carcinoma (SCC)

AntibodyApplication
Mouse anti-human SCC mAbFor immunodiagnostic: ELISA, LFA, CLIA
Humanized anti-human SCC mAb
Prokaryotic expression antigen (full length)

AntigenApplication
Prokaryotic expression antigen (full length)For immunodiagnostic: ELISA, LFA, CLIA

Neuron-Specific Enolase (NSE)

AntibodyApplication
Mouse anti-human NSE mAbFor immunodiagnostic: ELISA, LFA, CLIA

AntigenApplication
Recombinant human neuron-specific enolase (Prokaryotic NSE protein full length)For immunodiagnostic: ELISA, LFA, CLIA

Cytokeratin 19 Fragment (CYFRA21-1)
NameApplication
Mouse anti-human CYFRA21-1 mAbFor immunodiagnostic: ELISA, LFA, CLIA
Humanized anti-human CYFRA21-1 mAb

Human Progastrin-releasing Peptide (ProGRP Tumor Marker)

AntibodyApplication
Mouse anti-human ProGRP mAbFor immunodiagnostic: ELISA, LFA, CLIA
Humanized anti-human ProGRP mAb

AntigenApplication
Recombinant human proGRP 31-98 fragment (Prokaryotic expression)For immunodiagnostic: ELISA, LFA, CLIA

Protein Induced by Vitamin K Absence or Antagonist-II (PIVKA II Tumor Marker)
AntibodyApplication
Mouse anti-human PIVKA-II mAbFor immunodiagnostic: ELISA, LFA, CLIA
Humanized anti-human PIVKA-II mAb

AntigenApplication
Human PIVKA II protein prokaryotic expression (N-terminal fragment)For immunodiagnostic: ELISA, LFA, CLIA

Alpha-fetoprotein(AFP)
AntibodyApplication
AFP Rapid Quantitative Test Kit(CLIA)For immunodiagnostic: ELISA, LFA, CLIA
AMH Rapid Quantitative Test Kit (CLIA)

Tumor Antigens
Tumor antigens generally refer to new or overexpressed antigenic substances during the occurrence and development of tumors.

The possible mechanisms for the body to produce tumor antigens are: 1) gene mutation; 2) the activation of genes that were not expressed initially during the process of cell carcinogenesis; 3) abnormalities in some aspects of the antigen synthesis process (such as abnormal glycosylation leading to the production of unique protein degradation products) ); abnormal and ectopic expression of antigens or differentiation antigens during embryonic stage; ⑤ overexpression of specific gene products, significantly signal transduction molecules; ⑥ expression of exogenous genes (such as viral genes).
Cancer Antigen
According to the specificity of cancer antigens, cancer antigens are divided into (1) Tumor-specific antigens (2) Tumor-associated antigens.

Tumor-specific antigen (TSA) is a neoantigen-specific to tumor cells or exists only in specific but not in normal cells.

Tumor-associated antigen (TAA) refers to antigens peculiar to non-tumor cells and exist in normal cells and other tissues. Still, their content is significantly increased when cells become cancerous.

For more information about tumor markers list, please feel free to contact us!

As a professional tumor marker factory, SEKBIO has high-quality tumor marker for sale. Welcome to contact us if needed.

Triiodothyronine (T3)(2024年02月05日)

SEKBIO — 2024-02-26T15:47:20+0900

https://www.sekbio.com/products/triiodothyronine-t3-antibody/

Triiodothyronine, also known as T3, is a thyroid hormone. It affects almost every physiological process in the body, including growth and development, metabolism, body temperature, and heart rate.

The determination of triiodothyronine is one of the sensitive indexes in the diagnosis of hyperthyroidism.
When elevating hyperthyroidism, it is also the precursor of the recurrence of hyperthyroidism. In addition, pregnancy and acute hepatitis will advance Hypothyroidism, simple goiter, acute and chronic nephritis, chronic hepatitis, cirrhosis of the liver.
T3 can be used to diagnose t3 antibodies, early detection of hyperthyroidism, and diagnosis of pseudo thyrotoxicosis.

The serum T3 and T4 levels changes are generally consistent, which is a sensitive indicator of thyroid function diagnosis, especially important for early diagnosis. It is a specific diagnostic indicator of T3 hyperthyroidism.
Types Of Triiodothyronine (T3)
AntibodyApplication
Sheep Anti-human T3 mAbFor immunodiagnostic: ELISA, LFA, CLIA

Triiodothyronine Intro
As the largest endocrine gland in the human body, the thyroid secretes the main active substances, tetraiodothyronine (T4) and triiodothyronine (T3), which are extremely important for protein synthesis thermoregulation, energy production, and regulation effect.

T3 plays a vital role in judging the physiological condition of the human body, so it is of great significance to detect the content of T3 in serum. The content of T3 in serum is 1/80~1/50 of that of T4, but the biological activity of T3 is 5~10 times that of T4. Most of the T3 in serum is converted from the deiodination of peripheral tissues. A small part of T3 is directly secreted by the thyroid and released into the blood. Most of the T3 in serum is bound to binding proteins. About 90% is bound to thyroxine-binding globulin (TBG), the rest is bound to albumin, and a tiny amount is attached to thyroxine-binding prealbumin (TBPA).
Free T3 Test
Increased T3 is seen in hyperthyroidism, early stage of triiodothyronine-type hyperthyroidism crisis, iodine-deficiency goiter, and hyperthyroid-binding globulinemia.
It is decreased in hypothyroidism, low thyroxine-binding globulinemia, and so on.

High result possible disease: hyperthyroidism.

Low result possible diseases: Hypothyroidism, endocrine proptosis, thyroiditis, resting lymphocytic thyroiditis, hyperthyroidism in children, hyperthyroidism in the elderly, thyroid tuberculosis, endocrine disorders, euthyroid goiter, precocious children Breast hypertrophy.
TT3 Performance Data
ManufacturerSEKBIO
ParameterTT3
Linearity0.3-10 nmol/L
Detection limit0.3 nmol/L
Benchmark productRoche
Test principle and procedureTT3: Add 30 μl of sample + 30 μl of Rd, incubation for 10 minutes without cleaning, 30 μl of Ra + 30 μl of Rc and incubation for 5 minutes. Wash with 200 μl of washing solution. Finally, add 100 μl of A + 100 μl of B.

Precision
SampleRLUConcentrationSampleRLUConcentration

0.5270,9460.47

583,3364.10
276,8990.4579,4064.42
278,5360.4482,6694.16
259,5900.5284,4274.02
269,6900.4881,3634.26
270,2300.4779,2634.44
257,8370.5380,5654.33
270,8800.4777,3894.60
279,9360.4481,2334.27
271,2770.4779,1134.45
AVE270582.100.47AVE80876.404.30
SD7269.040.03SD2169.670.18
CV2.7%6.1%CV2.7%4.2%

Limit of detection
SampleRLUConcentration

Ag Diluent980,3051,022,44900
986,9631,001,87300
984,2091,003,97800
1,026,2521,000,04200
1,018,574961,33300
974,731982,27800
1,022,323969,06600
1,071,730968,48900
1,043,7241,028,07600
1,014,5951,014,79700
AVE1003789.350.00
SD28331.300.00
AVE+2*SD0

Linear
Sample（nmol/L)Dilution ratioConcentration valueAverage
10.131.010.4110.6710.1310.41
8.140.88.027.868.037.97
6.150.65.965.905.945.94
4.160.43.673.723.663.68
2.160.21.791.751.701.75
0.170.00.170.180.160.17

Method Comparison

Reagents Stability
ReagentSample(nmol/L)AverageDeviation
2-8℃9.1855,377
4.7181,863
0.43340,779
Antigen diluent666,737
random sample200,281
calf serum453,520
37℃ 1d9.1853,767-2.91%
4.7183,2581.70%
0.43339,943-0.25%
Antigen diluent664,104-0.39%
random sample188,472-5.90%
calf serum459,5291.32%
37℃ 3d9.1851,653-6.72%
4.7182,7141.04%
0.43360,8885.90%
Antigen diluent645,376-3.20%
random sample198,372-0.95%
calf serum480,3045.91%
37℃ 7d9.1849,977-9.75%
4.7178,711-3.85%
0.43366,3697.51%
Antigen diluent629,915-5.52%
random sample189,952-5.16%
calf serum486,1547.20%

Calibrator Stability
ReagentSample(nmol/L)AverageDeviation
2-8℃6.7043,902
3.7074,473
0.32310,740
37℃ 1d6.7043,667
3.7076,993-0.53%
0.32307,8313.38%
37℃ 3d6.7043,303-0.94%
3.7075,963-1.36%
0.32306,7062.00%

37℃ 7d
6.7044,214-1.30%
3.7072,4540.71%
0.32305,293-2.71%

As a professional tumor marker factory, SEKBIO has high-quality tumor marker for sale. Welcome to contact us if needed.

Thyroid Function(2024年02月04日)

SEKBIO — 2024-02-26T15:46:29+0900

https://www.sekbio.com/products/thyroid-function-antibody/

The thyroid gland is a 2-inches long, butterfly-shaped endocrine gland generally located in the lower front of the neck. The thyroid functions to make thyroid hormones, which are released into the blood and then carried to every tissue in the body. Thyroid hormones regulate the body using energy, maintaining body temperature, and keeping the brain, heart, muscles, and other organs working.

For this reason, an imbalance in triiodothyronine antibody hormone levels can negatively affect your bodily functions and your mood. Thyroid tests are used to diagnose thyroid function by detecting thyroid hormone markers in the blood. The specific blood tests to test your thyroid can include Thyroglobulin (Tg), Thyroid Peroxidase (TPO), Thyroid Stimulating Hormone Receptor (TSHR), Triiodothyronine (T3), etc.

Thyroid Function Products
Thyroid Stimulating Hormone Receptor (TSHR)
AntibodyApplication
Mouse Anti-human TSHR mAbFor immunodiagnostic: ELISA, LFA, CLIA
Humanized Anti-human TSHR mAb

AntigenApplication
Recombinant Human sTSHR (Soluble)For immunodiagnostic: ELISA, LFA, CLIA
Human TSHR Extractive of Cell Membrance (Full Length)

Triiodothyronine (T3)
AntibodyApplication
Sheep Anti-human T3 mAbFor immunodiagnostic: ELISA, LFA, CLIA

Thyroid Peroxidase (TPO)
AntibodyApplication
Humanized Anti-human TPO mAbFor immunodiagnostic: ELISA, LFA, CLIA

Thyroglobulin (Tg)
AntibodyApplication
Humanized anti-human Tg mAbFor immunodiagnostic: ELISA, LFA, CLIA

Thyroid Stimulating Hormone(TSH)
FT3(Free Triiodothyronine)
FT4(Free Thyroxine)
Thyroid Function Intro
The physiological function of the thyroid is mainly to promote the metabolism of three major nutrients, regulate growth and development, increase tissue oxygen consumption, promote energy metabolism, increase heat production and improve basal metabolism. When thyroid dysfunction occurs, hyperthyroidism or hypothyroidism will occur.

The hypothalamus-pituitary-thyroid axis mainly regulates the synthesis and secretion of thyroid hormones. Thyroxine (T4) and triiodothyronine (T3) are synthesized in thyroid follicular epithelial cells. Under the stimulation of pituitary thyroid-stimulating hormone (TSH), they are released into the blood after a series of changes.

All processes from iodine uptake to release in thyroxine synthesis have a promoting effect. More than 99% of T3 and T4 are bound to plasma proteins, mainly attached to thyroxine-binding globulin. Only about 0.4% of total plasma T3 and 0.04% of T4 are free, and only free T3 and T4 can Enter the target cells to play a role, and the part bound to the protein regulates and stabilizes free T3 and T4. The level of free T3 and T4 in the blood Fluctuating negative feedback causes an increase or decrease in the release of thyrotropin-releasing hormone (TRH) from the hypothalamus and the release of thyrotropin-releasing hormone (TSH) from the pituitary. The primary role of TRH is to promote the synthesis and release of TSH from the pituitary.
Thyroid Antibody
(1) Thyroglobulin antibody (TGA) and thyroid microsomal antibody (TMA): TGA and TMA in serum are two primary specific thyroid autoantibodies. TGA is a specific diagnostic indicator for chronic lymphocytic thyroiditis, often significantly elevated. Autoimmune thyroid disease TGA and TMA are elevated and can also be detected in the blood of other thyroid diseases and healthy people.

(2) Thyrotropin receptor antibody (TRAb): TRAb is also known as a thyroid-stimulating antibody (TSAb) or thyroid-stimulating immunoglobulin (TSI). TRAb is a kind of thyroid autoantibody produced in the autoimmune process of malignant diffuse goiter. It can stimulate the thyroid to produce thyroid hormones. The determination of TRAb is beneficial to the study of the pathogenesis of diffuse malignant goiter.

For more information about tshr blood test, please feel free to contact us!

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Squamous Cell Carcinoma (SCC)(2024年02月03日)

SEKBIO — 2024-02-26T15:45:55+0900

https://www.sekbio.com/products/squamous-cell-carcinoma-scc-tumour-marker/

Squamous cell carcinoma (SCC Tumor Marker) antigen is a 48kDa glycoprotein initially isolated from a squamous cancer of the uterine cervix.
Immunohistochemically, SCC is a cytoplasmic protein of normal and cancerous squamous cells.

Studies have indicated that SCC antigen is a good marker for diagnosis and monitoring the effectiveness of treatment of SCCs.

The clinical significance of SCC is as follows:
1. It has a high diagnostic value for cervical cancer
2. Assist in the diagnosis of lung squamous cell carcinoma
3. Prediction of esophageal squamous cell carcinoma
4. Diagnosis and monitoring of other squamous cell cancers (head and neck cancer, vulva cancer, bladder cancer, anal cancer, skin cancer, etc.)

Squamous Cell Carcinoma (SCC Marker) Products

SCC Tumor Marker
Squamous cell carcinoma, also known as epidermal carcinoma, is a malignant tumor that occurs in the epidermis or adnexal cells, and the cancer cells have different degrees of keratinization. It is more common in areas covered by squamous epithelium, such as the skin, mouth, lips, esophagus, cervix, vagina, etc. In addition, some parts such as the bronchus, bladder, renal pelvis, etc., although not covered by squamous epithelium, can form squamous cell carcinoma through squamous metaplasia.
SCC AG
Serum scc ag test assay has high specificity for diagnosing various squamous cell carcinomas. It is an effective and sensitive marker for squamous cell carcinomas of the vulva, vagina, and cervix. For primary cancer of the vulva and vagina, the sensitivity is 40%-50%; for primary cervical squamous cell carcinoma, the diagnostic sensitivity can reach 50%-70%; the sensitivity for the diagnosis of recurrent cancer can get 65%-85%. The serum SCCA in patients with cervical cancer is positively correlated with the clinical stage. The rise and fall of the SCCA level are consistent with the progression or improvement of the disease. Dynamic monitoring of serum SCCA levels can indicate cervical cancer disease monitoring. Serum SCCA levels in patients with cervical cancer rise again after treatment, or serum SCCA is negative before treatment. Elevated SCCA levels are earlier than clinical signs of recurrence or imaging suggestive of tumor recurrence.

It is worth noting that SCCA can also be found in normal body fluids such as sweat, saliva, and other body fluids. If such body fluids contaminate the specimen, even sneezing may pollute the specimen and cause false positives. Therefore, the sample with elevated SCCA should be repeated for determination.

For more information about scc antigen, please feel free to contact us!

As a professional tumor marker factory, SEKBIO has high-quality inflammatory marker for sale. Welcome to contact us if needed.

Others(2024年02月02日)

SEKBIO — 2024-02-26T15:45:20+0900

https://www.sekbio.com/products/secondary-antibody/

The first antibody is commonly said antibody, namely can and antigen-specific binding. The second antibody can combine with an antibody, namely the primary antibody and secondary antibody of antibody. It mainly detects the presence of antibodies. If the primary antibody is the antibody against the antigen, we can say that the secondary antibody is the antibody against the primary secondary antibody. That is, the antibody can also act as an antigen to stimulate the body to produce antibodies. In other words, when an antigen enters the body, it stimulates the body's immune system to produce an immune response, and B cells can produce specific proteins that bind specifically to the corresponding antigen.

Secondary Antibody Products
Streptavidin SA
Human IgM
AntibodyApplication
Mouse anti-human IgG monoclonal antibodyFor immunodiagnostic: ELISA, LFA, CLIA

Human IgG
AntibodyApplication
Mouse anti-human IgG monoclonal antibodyFor immunodiagnostic: ELISA, LFA, CLIA
Control Line
AntibodyApplication
Rabbit IgGFor immunodiagnostic: ELISA, LFA, CLIA
Mouse IgG
Mouse IgM
Chicken IgY
Goat Anti Mouse IgG
Goat Anti Rabbit IgG
Goat Anti Chicken IgY
Mouse Anti Human IgG

Additive
AntibodyApplication
Mouse anti-human RBCFor immunodiagnostic: ELISA, LFA, CLIA

Secondary Antibody Intro
The secondary antibody is an immunoglobulin against the antibody produced by the immune system of the xenogeneic animal by using the antigenic property of the protein as a macromolecule to immunize the xenogeneic animal. The secondary antibody is an antibody that can bind to the antibody, that is, the antibody, and its primary function is to detect the presence of the antibody and amplify the signal of the primary antibody. Anti-AIV H5 subtype hemagglutinin monoclonal antibody was used as the coating antibody, and rabbit anti-AIV IgG was used as the secondary antibody.
Primary and Secondary Antibody
A primary antibody is a protein that can specifically bind to a non-antibody antigen (specific antigen). Species include monoclonal and polyclonal antibodies.

Antibodies can also act as antigens to stimulate the body to produce antibodies. That is to say, the antigen enters the body to stimulate the body's immune system to produce an immune response, and B cells can produce particular proteins that specifically bind to the corresponding antigen. A primary antibody is an antibody against an antigen, and a secondary antibody is against the primary antibody. Both primary and secondary antibodies are groups that can specifically bind to other substances, and primary antibodies can secure at least two different groups (substrate and secondary antibody).

Secondary antibody: It can be combined with the primary and secondary antibody and has a label that can be detected (such as with a fluorescent, radioactive, chemiluminescent, or chromogenic group) and is used to detect the primary antibody. A secondary antibody is not required if the primary antibody has its detectable label (e.g., with a fluorescent, radioactive, chemiluminescent, or chromogenic group). But this is expensive because one primary antibody recognizes only one substrate. So today's design is generally a secondary antibody with a detectable label, and then the primary antibody is detected. The primary antibody recognizes the substrate. In this way, when the primary antibody binds to the substrate, it can be detected by the secondary antibody.

For more information about secondary antibody response and secondary antibody control, please feel free to contact us!

As a professional tumor marker factory, SEKBIO has high-quality inflammatory marker for sale. Welcome to contact us if needed.

Recombinant Human Neuron-specific Enolase (Prokaryotic NSE Protein Full Length)(2024年02月01日)

SEKBIO — 2024-02-26T15:44:37+0900

https://www.sekbio.com/products/recombinant-human-neuron-specific-enolase-prokaryotic-nse-protein-full-length-antibody/

Name: Recombinant human neuron-specific enolase (Prokaryotic NSE protein full length)
Catalog No.: ZLP113-1
Description: Recombinant protein
Epitope: Prokaryotic NSE protein full length

NameCatalog No.DescriptionEpitope
Recombinant human neuron-specific enolase (Prokaryotic NSE protein full length)ZLP113-1Recombinant proteinProkaryotic NSE protein full length

For more information about nse recombinant antibody and cardiac markers blood test, please feel free to contact us!

As a professional tumor marker factory, SEKBIO has high-quality infectious markers for sale. Welcome to contact us if needed.

Recombinant COVID-19 Spike 1 His Full Length Protein(2023年12月07日)

SEKBIO — 2023-11-22T16:52:49+0900

https://www.sekbio.com/products/recombinant-covid-19-spike-1-his-full-length-protein-antibody/

Catalog #: HP811-16
Description: Recombinant Protein, research use only
Molecular weight: 29KD, theoretically

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As a professional tumor marker factory, SEKBIO has high-quality tumor marker for sale. Welcome to contact us if needed.

Protein Induced by Vitamin K Absence or Antagonist-II (PIVKA II Tumor Marker)(2023年12月06日)

SEKBIO — 2023-11-22T16:52:19+0900

https://www.sekbio.com/products/protein-induced-by-vitamin-k-absence-or-antagonist-tumor-marker/

In the absence of vitamin K, liver cells cannot depend on vitamin K in the synthesis of standard clotting factors, only the synthesis of no function of blood coagulation abnormal prothrombin (DCP) -- pivka marker, is the protein induced by vitamin K deficiency or antagonist - II, can appear in vitamin K deficiency or the serum of patients with hepatocellular carcinoma (HCC).

At present, Chinese and foreign guidelines have listed PIVKA II antibody as a significant indicator for liver cancer detection.
Pivka-ii has been written into the guidelines by the Asian-Pacific Society of Hepatology and the Japanese Society of Hepatology and recommended high-risk screening populations as an auxiliary diagnosis of liver cancer, monitoring treatment effect, and prognostic and recurrence prediction tool.
PIVKA II Intro
Pivka ii antibody is a vitamin K deficiency or antagonist-II-induced protein, also known as abnormal prothrombin (Des-gamma-carboxy prothrombin, DCP). Normal liver in vitamin K Prothrombin is produced under the action. Still, abnormal prothrombin is made in patients with vitamin K deficiency or hepatocellular carcinoma. Its serum level is in patients with vitamin K deficiency, patients treated with warfarin, or patients with hepatocellular carcinoma (HCC). It will be elevated in serum and can be used to diagnose HCC.
pivka blood test
PIVKA 2 is used to diagnose hepatocellular carcinoma, which is helpful for the screening of high-risk groups. It is helpful for the auxiliary diagnosis of hepatocellular carcinoma when combined with imaging such as ultrasound detection. The serum half-life of PIVKA-II is about 40 to 72 hours, which is shorter than that of AFP. 3 to 5 days can more timely reflect the efficacy of HCC.

As a professional tumor marker factory, SEKBIO has high-quality tumor marker for sale. Welcome to contact us if needed.

Prostate-Specific Antigen (PSA Tumor Marker)(2023年12月05日)

SEKBIO — 2023-11-22T16:50:11+0900

https://www.sekbio.com/products/prostate-specific-antigen-psa-tumour-marker/

Prostate-specific antigen （PSA specific antigen）, also known as gamma-selenoprotein or kallikrein-3, is a glycoprotein enzyme encoded in humans by the KLK3 gene. PSA is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland.

The PSA tumour marker test can detect high levels of PSA that may indicate the presence of prostate cancer. However, many other conditions, such as an enlarged or inflamed prostate, can also increase PSA levels. Therefore, determining what a high PSA score means can be complicated.

SEKBIO now offers Mouse anti-human PSA mAb, Humanized anti-human PSA mAb, Mouse anti-human free PSA mAb, and Prostate-Specific Antigen (Eukaryotic expression).

PSA Tumour Marker Intro
PSA is a single-chain polypeptide containing 237 amino acids. It belongs to the serine protease family with tissue-specific chymotrypsin-like action. It can decompose the main gelatinous protein in semen and has the effect of diluting semen.

An inactive zymogen (pro-PSA) is initially secreted into the prostate duct is an inactive zymogen (pro-PSA), which forms an active prostate-specific antigen after cleaving seven amino acids at the amino terminus. PSA is synthesized in both standard and cancer-like epithelial cells. Most prostate-specific antigens entering the blood circulation are rapidly combined with proteolytic enzyme inhibitors, mainly with α-1 antichymotrypsin (ACT) and α-2 macroglobulin (MG) proteolytic enzymes inactivate some. exist in a free state. PSA is tissue-specific and only exists in the cytoplasm of human prostate acinar and duct epithelial cells and is not expressed in other cells. However, it is not tumor-specific, and prostatitis, benign prostatic hyperplasia, and prostate cancer can lead to elevated total PSA levels (free PSA plus composite PSA).
PSA Tumor Marker Testing
The traditional test, digital rectal examination (DRE), sometimes fails to detect small tumors confined to the prostate. In 1990, Cooner et al. published the research results about the application of prostate ultrasound examination and detection of serum PSA in the diagnosis of early prostate cancer, abnormal DRE, and PSA can significantly improve the prediction of the prostate. Therefore, the combined detection of DRE and PSA can improve the early prostate cancer diagnosis rate. Detection of PSA in serum is another valuable prostate diagnosis method besides ultrasonography and digital rectal examination and the most accurate way among the three. Patients easily accept this test, and its results are quantitative and objective and are not affected by the operator's technique. For prostate cancer patients who have undergone surgery or other treatments, the detection of PSA is also very meaningful for detecting tumor metastasis and recurrence. After treatment, if the patient has persistent PSA elevation or re-increase again, it indicates that the swelling remains or recurs.

As a professional tumor marker factory, SEKBIO has high-quality tumor marker for sale. Welcome to contact us if needed.

N-terminal Pro B Type Natriuretic Peptide (NT-proBNP)(2023年12月04日)

SEKBIO — 2023-11-22T16:49:39+0900

https://www.sekbio.com/products/n-terminal-pro-b-type-natriuretic-peptide-nt-probnp/

NT-proBNP is a product released under the pressure load of the heart, and its level can reflect the myocardial function and damage degree, which is related to the severity of heart failure. It is one of the most clinically used heart failure biomarkers.

N terminal probnp has essential value in the clinical diagnosis, risk stratification, prognosis evaluation, and curative effect monitoring of patients with acute heart failure (AHF) and chronic heart failure (CHF).

This precursor molecule is cleaved to form the active BNP and the inactive probnp n terminal-BNP (NT-proBNP) fragment. Myocardial cells secreted NT-proBNP in response to increase volume and pressure. Comparisons of BNP and NT-proBNP have shown that both eight molecules effectively diagnose left ventricular dysfunction in the acute care/emergency setting.
NT-proBNP Intro
Serum marker detection is an important means for the early diagnosis and prognosis of heart failure in clinical practice. The heart failure guidelines recommend the preferred serum markers for heart failure. Among them, n terminal pro b type natriuretic peptide (a neuroendocrine system activation marker), including brain natriuretic peptide (BNP) and amino-terminal brain natriuretic peptide (NT-proBNP), are the most popular at home and abroad (ECS/ACC/AHA/HFSA/CSC). It is recommended to use natriuretic peptides to identify high-risk heart failure groups and comprehensively manage and prevent them.

Compared with BNP, NT-proBNP has a longer half-life and better stability, so NT-proBNP has higher sensitivity for detecting early or mild heart failure, and it takes more time for blood samples to be sent to the laboratory making it possible more suitable for clinical applications.
NT-proBNP Test
Because NT-proBNP has a long half-life and is relatively more stable, the specimens can be stored and detected either in the central laboratory or at the bedside, while BNP has a short half-life and poor stability and can only be detected immediately at the bedside. NT-proBNP is slowly cleared in the blood and can accumulate at a higher concentration, reflecting the state and changes of cardiac function more sensitively to a certain extent, especially in the early diagnosis of heart failure. In addition, NT-proBNP has no physiological activity and is not affected by recombinant human brain natriuretic peptides (such as neoactivin). For patients treated with recombinant human brain natriuretic peptides, it is recommended to prefer NT-proBNP to evaluate the changes in cardiac function. BNP is cleared by binding to BNP clearance receptors neutral endonucleases, and a small part of it is emptied by renal metabolism. NT-proBNP is mainly removed by renal metabolism, so its concentration is more significantly affected by renal function than BNP.

As a professional tumor marker factory, SEKBIO has high-quality inflammatory marker for sale. Welcome to contact us if needed.

Mouse Myo mAb(2023年12月03日)

SEKBIO — 2023-11-22T16:49:12+0900

https://www.sekbio.com/products/mouse-myo-mab-antibody/

Myo antibody
Name: Myo mAbMouse mAb
Catalog No. GSA501
Description: Epitope Mouse

SEKBIO IVD Raw Materials Services
As a diagnostic antigen/antibody manufacturer, we provide a full range of IVD antigens/antibodies, and we owe a veteran team with 30 + years of experience in the IVD industry. Please feel free to let us know your requirement.

As a professional tumor marker factory, SEKBIO has high-quality inflammatory marker for sale. Welcome to contact us if needed.

Mouse Anti-human ProGRP mAb(2023年12月02日)

SEKBIO — 2023-11-22T16:47:50+0900

https://www.sekbio.com/products/mouse-anti-human-progrp-mab-antibody/

Pprogrp antibody
Name: Mouse anti-human ProGRP mAb
Catalog No.: ZLA116-1M
Description: Mouse mAb
Epitope: 48-52

As a professional tumor marker factory, SEKBIO has high-quality infectious markers for sale. Welcome to contact us if needed.

Influenza (Flu)(2023年12月01日)

SEKBIO — 2023-11-22T16:45:21+0900

https://www.sekbio.com/products/influenza-flu-antibody/

Influenza virus is the representative species of the Orthomyxoviridae family. The typical clinical symptoms of these diseases are acute high fever, general pain, marked fatigue and respiratory symptoms.
Influenza viruses are spread mainly through airborne droplets, contact between a susceptible person and an infected person, or contact with contaminated objects.

The General autumn-winter season is its high incidence period. Influenza A and B viruses mainly caused human influenza.
The rapid diagnosis of Flu is using the immunofluorescence method, ELISA method to detect virus antigen.

We usually take the patient's nasal mucosa or respiratory tract exfoliated epithelial cell smear and see the antigen by immunofluorescence staining with fluorescin-labelled influenza virus immune serum. Or test the antigen in the patient's pharyngeal gargle by ELISA.

SEKBIO provides Mouse Flu A NP mAb, Mouse Flu B NP mAb, Flu A NP eukaryotic expression recombinant protein and Flu B NP eukaryotic expression recombinant protein for Influenza diagnosis.
Flu Antibody
Influenza viruses are divided into three types: A, B, and C. Influenza A virus can cause a worldwide pandemic. Influenza virus is a segmented single-stranded negative-stranded RNA virus. The protein combined with nucleic acid is nucleoprotein (nucleoproteiN, NP). The NP antigen is stable and type-specific. Outside the virus, the core is the matrix protein (M protein) tightly bound to the virus envelope, and the outermost is the virus envelope. There are two kinds of spines on the envelope; one is hemagglutinin (HA), the other is nerve Aidase (neuraminidase, NA), which are easily mutated.

Serological methods for detecting antibodies include the hemagglutination inhibition test, complement fixation test, neutralization test, ELISA, etc., but the most commonly used is the hemagglutination inhibition test.

Antibodies for flu
Influenza virus can cause infection and disease in humans, birds, pigs, horses, bats, and other animals and is the pathogen of human and animal diseases such as human influenza, avian influenza, swine influenza, and equine influenza.

The typical clinical symptoms of these diseases are acute high fever, generalized pain, marked fatigue, and respiratory symptoms. Influenza viruses are mainly spread through airborne droplets, contact between susceptible and infected persons, or contaminated items. Generally, autumn and winter are the high incidence period. Human influenza is mainly caused by influenza A and B viruses.

Direct and indirect immunofluorescence, radioimmunoassay, ELISA, immunoelectron microscopy, etc., to detect antigens quickly, but indirect immunofluorescence and ELISA are the most commonly used. In addition, the PCR method and nucleic acid hybridization method can also use to check nucleic acid.

As a professional tumor marker factory, SEKBIO has high-quality bone marker for sale. Welcome to contact us if needed.

Anti-CA15-3 mAb(2023年07月20日)

SEKBIO — 2023-07-20T12:20:38+0900

https://www.sekbio.com/anti-ca15-3-mab.html

Cancer antigen 15-3 is a glycoprotein whose antigenic determinant is part sugar and part peptide. Cancer antigen 15-3 is an antigen associated with breast cancer and other malignant tumors. The level of CA15 3 antibody in the serum of breast cancer patients significantly increased, so it plays an essential auxiliary role in diagnosing breast cancer. Similar to the status of cancer antigen 50, CA15-3 is also a highly important cancer antigen, which is the best indicator for breast cancer patients to diagnose and monitor postoperative recurrence and observe the curative effect.

As a ca153 marker, we will do our best to meet all the needs of customers.

SEKBIO is an immunodiagnostic ivd raw materials manufacturer committed to making diagnostics more accurate.

Estradiol (E2)(2023年07月20日)

SEKBIO — 2023-07-20T12:19:27+0900

https://www.sekbio.com/products/estradiol-e2-antibody/

Estradiol E2 / E2 Estradiol
Estradiol, also spelled oestradiol, is an estrogen steroid hormone and the primary female sex hormone.
It is involved in the regulation of the estrous and menstrual female reproductive cycles.

Estradiol is a steroid estrogen. There are two types of estrogen, alpha, and beta, which are the most abundant and the most active. Granulosa cells secreted E2 in ovarian follicles. Its metabolites are estrone and estriol, which have 18 carbons.
The target organs are the uterus, vagina, fallopian tube, and pituitary gland.
Estrogen prompts cells to make DNA, RNA, and various proteins in their tissues and is involved in regulating the estrous and menstrual female reproductive cycles.

Estradiol (E2) Products

AntibodyApplication
Humanized Anti-human E2 mAbFor immunodiagnostic: ELISA, LFA, CLIA

Estradiol Intro
Estradiol is a steroidal estrogen. There are two types, α and β, and the α type has a solid physiological effect. Winterstein et al. have been extracted from the urine of pregnant horses and can also be obtained from the urine of pregnant women, human placenta, and pig ovaries. Also present in the testes or urine of male horses. Because it has an intense sex hormone action, it is believed that it or its ester is the essential sex hormone secreted by the ovaries.
E2 Testing
Estradiol antibody (estradiol) has two types, α, and β. It is a kind of estrogen with the highest content and the most vigorous activity. It is secreted by the granulosa cells of the follicles in the ovary. Its metabolites are estrone and estriol, and it contains 18 carbon atoms.

Estrogen can induce cells to synthesize DNA, RNA, and various proteins in corresponding tissues. Its target organs are the uterus, vagina, fallopian tubes, and pituitary gland. It can be used as transdermal estrogen therapy supplements the lack of 17-beta estradiol secreted by women's ovaries while avoiding side effects caused by oral administration (breast pain, weight gain, hypertension, gallstones, abnormal liver function, etc.).

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Dengue(2023年07月20日)

SEKBIO — 2023-07-20T12:18:59+0900

https://www.sekbio.com/products/dengue-antibody/

Dengue fever is a viral infectious disease that mainly occurs in tropical regions. Spread by Aedes species mosquito, Dengue virus infects millions of people every year.

Dengue viruses can divide into 1-4 serotypes based on the antigenicity of viral envelope protein E. Antigenic determinants of viral envelope protein E can induce the production of protective neutralizing antibodies and hemagglutination suppressive antibodies and may be involved in the development of Dengue hemorrhagic fever (DHF) and Dengue shock syndrome (DSS).

SEKBIO developed multiple antigens and antibodies for Dengue fever diagnostics, including Dengue NS1 Antibody, Recombinant Dengue Type I NS1 protein, Recombinant Dengue Type II NS1 protein, Recombinant Dengue Type III NS1 protein, Recombinant Dengue Type IV NS1 protein.

Dengue Products
AntibodyApplication
Dengue NS1 AntibodyFor immunodiagnostic: ELISA, LFA, CLIA

AntigenApplication
Recombinant Dengue Type I NS1 ProteinFor immunodiagnostic: ELISA, LFA, CLIA
Recombinant Dengue Type II NS1 Protein
Recombinant Dengue Type III NS1 Protein
Recombinant Dengue Type IV NS1 Protein

Dengue Antibody Test
Dengue fever is an acute insect-borne infectious disease caused by the mosquito-borne dengue virus. Dengue virus infection can lead to latent infection, dengue fever, and dengue hemorrhagic fever. The typical clinical manifestations of dengue fever are rapid onset, high fever, headache, severe pain in muscles, bones, joints, skin rash, bleeding tendency, swollen lymph nodes, decreased white blood cell count, and thrombocytopenia in some patients. Dengue hemorrhagic fever is rare in my country.

Using ELISA to detect specific IgM antibodies in the serum of patients, positive results are helpful for the early diagnosis of dengue fever. If the dengue virus antigen is caught in the patient's serum, it can also be used as the basis for a definite diagnosis.
Dengue Antigen
According to the different antigenicity of viral envelope protein E, the dengue virus is divided into 1 to 4 serotypes. The antigenicity of each type of virus is crossed, but there is no crossover with other antigenic groups of Flaviviridae. The epitope of viral envelope protein E can induce the host to produce protective neutralizing antibodies and hemagglutination-inhibiting antibodies and may be involved in the occurrence of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The nonstructural proteins NS1 and NS3 are both immunoreactive and immunogenic and can induce protective immunity in mice against lethal challenge with homotypic dengue virus. NS1 is a soluble complement-binding antibody with type- and group-specific dengue virus epitopes.

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Cytokeratin 19 Fragment (CYFRA21-1)(2023年07月20日)

SEKBIO — 2023-07-20T12:18:25+0900

https://www.sekbio.com/products/cytokeratin-19-fragment-cyfra21-1-tumor-marker/

Cytokeratins are epithelial markers whose expression don't lose during malignant transformation. CYFRA21 1 is a cytokeratin-19 fragment soluble in serum and can use as a circulating tumour marker. Although expressed in all body tissues, it is significant in the lung, particularly lung cancer tissues. CYFRA21 1 is a sensitive and specific tumour marker of non-small-cell lung cancer (NSCLC), especially the squamous cell subtype.It also reflects the extent of the disease and has an independent prognostic role along with performance status and disease stage in NSCLC.In addition, detection of serum CYFRA 21–1 allows for identifying high-risk patients that may benefit from adjuvant chemotherapy and enables the early detection of progressive disease in recurrent NSCLC.Additionally, CYFRA 21–1 describe as a valuable marker for oesophagal squamous cell carcinoma and therapy monitoring of bladder cancer.

CYFRA21-1 IntroIn malignant epithelial cells, the activated protease accelerates the degradation of the cells so that a large number of cytokeratin 19 fragments are released into the blood, and the soluble pieces can specifically bind to the two monoclonal antibodies KS19.1 and BM19.21, so it is called CYFRA21 -1. In malignant lung cancer tissues, CYFRA21-1 is abundant, especially in lung squamous cell carcinoma. The molecular weight of CYFRA21-1 is about 30,000 Da.The serum level of CYFRA21-1 was not related to age, gender, smoking, and pregnancy. Pneumonia, tuberculosis, bronchitis, bronchial asthma, emphysema, and other diseases generally do not cause CYFRA21-1 elevation. Benign liver disease renal failure can cause a slight increase, but rarely more than 10ng/ml. Cytokeratin Tumor Marker Cytokeratin (CK) classifies intermediate filaments that constitute the cytoskeleton. There are many types, among which the cytokeratin 19 (CK19) fragment CYFRA21-1 is the most important in the diagnosis of malignant tumors; the release of CK19 increases when cells become cancerous. , After CK19 is degraded by protease or apoptosis, its fragments are released into the blood. The expression of different keratin fragments has the specificity of tissue distribution, squamous cell carcinoma and adenocarcinoma mainly express CK19, small cell undifferentiated carcinoma mainly express CK18 or only a tiny amount of CK19. CYFRA21-1 is a fragment of cytokeratin 19. It is composed of two monoclonal antibodies to cytokeratin 19, the main component of the cell structure. It is mainly found in the cytoplasm of tumor cells of epithelial origin, such as lung cancer and esophageal cancer. All lung squamous cell carcinomas express CYFRA21-1. When the cells become cancerous, the release of CYFRA21-1 due to the necrolysis of tumor cells increases the blood content, reflecting the prognosis and therapeutic efficacy of the disease. Since the serum product of CK19, CYFRA21-1, is a non-organ-specific tumor marker, comprehensive analysis of clinical data should be combined in clinical application to make a more accurate judgment.

SEKBIO Incorporated is a prestigious biotech developer and manufacturer, based in the vibrant city of Shenzhen. We pride ourselves on being your trusted partner in the field of Immunodiagnostic reagents, delivering innovative solutions to meet your needs. Our diverse range of cutting-edge immunoassay platforms includes fully automatic chemiluminescence immunoassay, fluorescent immunoassay, and LFA, ensuring accurate and reliable results.

Heart-type Fatty Acid Binding Protein (H-FABP)(2023年07月20日)

SEKBIO — 2023-07-20T12:17:53+0900

https://www.sekbio.com/heart-type-fatty-acid-binding-protein-h-fabp.html

H-FABP is a low-molecular-weight cytosolic protein involved in the intracellular uptake and buffering of fatty acids in the myocardium. It is expressed primarily in the heart, but it is not 100% cardio specific since it is expressed to a lesser extent in skeletal muscle, brain, and kidneys. The H-FABP level in serum is elevated rapidly during myocardial injury, making it an ideal marker for myocardial infarction. It is a useful prognostic marker in patients with proven acute coronary syndrome. However, renal failure and skeletal muscle disease have limited diagnostic value for AMI.

Details
Fatty heart type fatty acid binding proteins (FABPs) are small cytoplasmic proteins abundantly expressed in tissues with an active fatty acid metabolism, such as the heart and liver. Nine distinct types of the FABP family have been identified, with Heart-type FABP (H-FABP) being the most widely studied, primarily because it is abundant in the cardiomyocytes.

H-FABP concentrations typically peak at approximately 6-8 hours after symptoms of acute coronary syndrome (ACS) and return to normal within 24-30 hours. Although H-FABP has similar release kinetics to Myoglobin (i.e. rapid release & clearance), it is approximately 15-20 times more cardiac-specific, making it a vastly more productive biomarker for myocardial injury. Furthermore, the average serum/plasma value of H-FABP is much lower than with Myoglobin, which reduces the potential for false-positive results.

Clinical Value:

1. Diagnosis of AMI: 30min~3h after the onset of AMI, plasma FABP begins to increase and returns to normal within 12h~24h, so FABP is one of the early diagnostic indicators of AMI. Its sensitivity was 78%, significantly higher than Mb and ckmb protein. Therefore, FABP is more valuable for early diagnosis of AMI than Mb and CK-MB.

2. Others: Plasma FABP can also be increased in patients with skeletal muscle injury and renal failure.

Reference value: <5ug/L

Catalog No.Product NameClone
ZLA1041MMouse anti-human FABP mAb9C5M
ZLA1042MMouse anti-human FABP mAb5D9M
ZLA1042HHumanized anti-human FABP mAb5D9H
ZLP1041Recombinant Antigen (H-FABP)Antigen

Sekbio | FDA Grade Antibodies
Sekbio offers FDA BfArM grade antibodies, LFA uncut sheets, CLIA bulk reagents and customized development services.
We supply mAbs to the top 3 Chinese IVD brands with our 2000 ㎡ GMP-grade facility.

We can also offer kinds of cardiac markers blood test for sale, if you have needs, please contact us.

Cardiac troponin I (cTnI)(2023年07月20日)

SEKBIO — 2023-07-20T12:17:08+0900

https://www.sekbio.com/cardiac-troponin-i-ctni.html

Cardiac troponin I, often denoted as cTnI, is presented in cardiac muscle tissue by a single isoform with a molecular weight of 23.9 kDa. It consists of 209 amino acid residues. The theoretical pI of cTnI is 9.05. Phosphorylation of cTnI changes the conformation of the protein and modifies its interaction with other troponins and the interaction with anti-TnI antibodies. These changes alter the myofilament response to calcium and are of interest in targeting heart failure. Multiple reaction monitoring of human cTnI has revealed 14 phosphorylation sites, and the pattern of phosphorylation observed in these sites is changed in response to disease. Ctni antibody is considered more sensitive and significantly more specific in the diagnosis of myocardial infarction than the "golden marker" of last decades – ckmb protein, as well as total creatine kinase, myoglobin (myo antibody), and lactate dehydrogenase isoenzymes.

The comparison study between Sekbio's Ab and Abbott hs-cTnL and Ortho VITROS system Coated Ab: HA101-1MConjugated Ab: HA101-2H

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Antibody for COVID-19 antigen test(2023年07月20日)

SEKBIO — 2023-07-20T12:16:25+0900

https://www.sekbio.com/antibody-for-covid-19-antigen-test.html

The antibodies pair (ZLA81120M, ZLA81122H) has been evaluated by some diagnostic pct antigen companies and successfully developed into a COVID-19 detection kit.

To help our clients fight the COVID-19 disease, SEKBIO introduces new raw material pairs to accelerate the development and production of ivd antigen test reagents.The antibodies pair (ZLA81120M, ZLA81122H) has been evaluated by some diagnostic reagent companies and successfully developed the COVID-19 detection kit.If you are interested in our products, please contact us for details. COVID-19 antigen test (LFA) Based on antibodies pair:1) Monoclonal antibody, CAT. No. ZLA81120M,coating2) Monoclonal antibody, CAT. No. ZLA81122H, conjugating

Procalcitonin PCT(2023年06月08日)

SEKBIO — 2023-05-22T15:27:49+0900

https://www.sekbio.com/procalcitonin-pct.html

Procalcitonin is a peptide precursor of the hormone calcitonin, the latter being involved with calcium homeostasis. It arises once preprocalcitonin is cleaved by endopeptidase.

Details

Current targeting conventional markers(C-reactive protein, white blood cell, tumor necrosis factor-α, interleukins, etc.) are non-specific for diagnosing sepsis. Procalcitonin (PCT), a member of the calcitonin superfamily, could be a critical tool for diagnosing sepsis. But to distinguish between bacterial versus viral infections, procalcitonin alone may not be effective. Rapid elevation in the concentration of procalcitonin and other newly emerging biomarkers during disease and its correlation with severity of illness makes it an ideal biomarker for bacterial infection. Besides this, the procalcitonin levels can be used to monitor response to antimicrobial therapy, diagnose secondary inflammations, diagnose renal involvement in pediatric urinary tract infection, etc.

Sekbio provides procalcitonin metabolism, pct protein, pct antibody, thyroid antibody, etc. For more information, please feel free to contact us!

What is Human Epididymis Protein 4 (HE4)?(2023年06月07日)

SEKBIO — 2023-05-22T15:27:19+0900

https://www.sekbio.com/what-is-human-epididymis-protein-4-he4.html

1. What is he4 protein (HE4)?

HE4, called he4 human epididymis protein 4, is a sensitive indicator for detecting ovarian cancer and is one of the tumor markers. It is another highly recognized tumor marker for epithelial ovarian cancer after CA125. Human epididymis protein 4 (HE4) is not expressed in the normal ovarian surface epithelium, while it is significantly highly expressed in serous ovarian cancer and endometrioid ovarian cancer.

Compared with the glycoantigen CA125, which is less disturbed by external factors, CA125 is generally disturbed by many factors such as inflammation, endometriosis, and pregnancy, which can cause elevation, whereas human epididymis protein 4 (HE4) is generally not affected by these factors. The combined test of human epididymis protein 4 (HE4) and CA125 is more meaningful for detecting ovarian cancer.

Human epididymis protein 4 (HE4) combined with CA125 has high clinical value in the early diagnosis of epithelial ovarian cancer, disease monitoring, postoperative recurrence monitoring, as well as in differential diagnosis of benign tumors. Human epithelial protein (HE4) also has some sensitivity in the diagnosis of endometrial cancer. It is also seen in other tumors, such as the lung, breast, and pancreas. It is also seen in the increase of human epididymis protein 4. It is also increased in some gynecological inflammatory diseases, ovarian cysts, and other benign tumors.

2. Clinical significance of human epididymis protein 4 (HE4)

Human epididymis protein 4 (HE4) is expressed at high levels in ovarian cancer tissues and serum, as well as in endometrial, lung, colon, and breast cancers. Increased percentage of detectable serum human epididymis protein 4 (HE4) levels have been used as an aid to monitor the recurrence and progression of disease in patients with invasive epithelial ovarian cancer.

Elevated levels of HE4 may also be seen in other malignant disorders such as breast cancer, endometrial cancer, and, rarely, benign disorders

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The Bottleneck in the R&D of IVD Raw Materials Needs to Be Broken Through and the Competitiveness of the Industry Needs to Be Improved(2023年06月06日)

SEKBIO — 2023-05-22T15:26:49+0900

https://www.sekbio.com/the-bottleneck-in-the-rd-of-ivd-raw-materials-needs-to-be-broken-through-and-the-competitiveness-of-the-industry-needs-to-be-improved.html

1. Development of China's IVD raw materials industry

As China's ivd reagent raw materials industry started late, the ivd raw materials market is highly fragmented, and brand trust is low, there are still many difficulties in realizing domestic substitution. Compared with overseas IVD raw material enterprises, China's IVD raw material brands need to advance in R&D capabilities and production technology.

2. Global market development of IVD raw materials

At present, the global IVD raw material market as a whole presents the characteristics of many participants, small individual scale, and insufficient industry concentration. Even the leading companies have a relatively low global market share. It is challenging for a single company to include comprehensive product lines. The IVD raw material industry involves many fields and technology platforms. Therefore, IVD raw material companies can establish a complete and rich product matrix through endogenous development and M&A expansion, covering a variety of application scenarios and better meeting the diverse needs of downstream customers.

In addition, the IVD raw material industry has high brand barriers. To ensure product quality, IVD manufacturers usually spend a lot of time and money in the research and development stage to verify the reliability of raw materials. Therefore, many production enterprises choose core raw material manufacturers with long operating years and high product popularity, and customer stickiness continues to increase. Thus, insisting on good word-of-mouth marketing and customer service, and gradually building a brand image with market influence, is also a way for enterprises to achieve breakthrough development.

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Soluble Fms-like tyrosine kinase-1 (sFlt-1)(2023年06月05日)

SEKBIO — 2023-05-22T15:25:46+0900

https://www.sekbio.com/soluble-fms-like-tyrosine-kinase-1-sflt-1.html

Soluble forms-like tyrosine kinase-1 is a tyrosine kinase protein with antiangiogenic properties. A non-membrane-associated splice variant of VEGF receptor 1, sFlt-1 binds the angiogenic factors VEGF and PlGF, reducing blood vessel growth by reducing free VEGF and PlGF concentrations. sFlt-1/PLGF ratio is valuable for assessing the severity of preeclampsia and diagnosing early-onset preeclampsia and can be used to predict neonatal birth weight.

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Parathyroid Hormone (PTH)(2023年06月04日)

SEKBIO — 2023-05-22T15:25:19+0900

https://www.sekbio.com/parathyroid-hormone-pth.html

Parathyroid hormone, also called parathormone or parathyrin, is a hormone secreted by the glands that regulate the serum calcium concentration through its effects on bone, kidney, and intestine.

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Ovarian Cancer Detection Index: Human Epididymis Protein 4 (HE4)(2023年06月03日)

SEKBIO — 2023-05-22T15:24:45+0900

https://www.sekbio.com/ovarian-cancer-detection-index-human-epididymis-protein-4-he4.html

As the most common malignant tumor of female reproductive organs and the first cause of death from gynecological malignancies in China, ovarian cancer has seen a 30% increase in incidence and an 18% increase in mortality over the past 10 years. Because early symptoms are not obvious and early tumors are difficult to detect during pelvic examinations, 70% to 75% of ovarian cancer patients are already in advanced stages when diagnosed, which is why ovarian cancer is called the "silent killer".

Human epididymis protein 4 ovarian cancer(HE4) is a new tumor marker that can help in early diagnosis, differential diagnosis, treatment monitoring, and prognosis assessment of ovarian cancer. It is a real benefit for women's health.

1. What is human epididymis protein 4 (HE4)?

Human epididymis protein 4 belongs to the whey acidic 4-disulfide center (WFDC) protein family, which has suspected trypsin inhibitor properties. Other proteins in this family include SLPI, Elafin, and PS20 (WFDC1). The HE4 gene encodes a segment of 13 kD in length, although, at the time of its mature glycosylation formation, this protein is roughly 20 kD to 25 kD and contains a single chain with two WFDC structural domains.

In a comparative study of ovarian cancer patients with healthy states, Hellstrom et al. found that HE4 was up to 95% specific and 67% sensitive for detecting ovarian cancer. The specificity was significantly higher than that of CA125 alone. Combined with CA125, the test was more accurate in predicting ovarian cancer, with a sensitivity and specificity of 76% and 95%, respectively.

2. Application of human epithelial protein 4 (HE4) test in ovarian cancer patients

Ovarian cancer is one of the common malignant tumors threatening women's health. 70% of the patients are already in the advanced stage when diagnosed, and the five-year survival rate of advanced-stage patients is only 20% to 30% despite tumor cytoreductive surgery and chemotherapy. At present, the diagnosis of ovarian cancer mainly relies on clinical symptoms, pelvic examination, vaginal ultrasound, CT/MRI, and serum tumor markers.

The commonly used serum markers include CA125, HCG, CEA, TPA, etc. Especially CA125 is the most widely used and plays an important role in clinical diagnosis, chemotherapy monitoring, and follow-up; however, due to its low specificity, it has little significance in the early screening of ovarian cancer. Therefore, there is a lack of effective means for early diagnosis of ovarian cancer in clinical practice. And the discovery of HE4 assisted in solving this problem to a great extent.

3. Application of human epithelial protein 4 (HE4) test

(2) Disease status monitoring of patients diagnosed with ovarian cancer.

Several studies have demonstrated that changes in serum HE4 concentration levels in ovarian cancer patients are positively correlated with changes in disease progression. Therefore, the progression of ovarian cancer can be assessed by continuous measurement of serum HE4 concentration levels.

(3) Note on limitations of application:

HE4 test levels may be at normal values in some patients with diagnosed ovarian cancer and may be seen in mucin-type ovarian cancer or germ cell-type ovarian cancer, both of which rarely express human epithelial protein 4 (HE4);

The concentration level of HE4 is not absolute evidence for the presence or absence of malignancy;

The rules of risk assessment for malignant ovarian cancer do not apply to patients undergoing chemotherapy and those younger than 18 years of age.

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Myo - The earliest abnormally increased cardiac protein marker after myocardial injury(2023年06月02日)

SEKBIO — 2023-05-22T15:24:13+0900

https://www.sekbio.com/myo---the-earliest-abnormally-increased-cardiac-pr.html

Myoglobin (Myo) is a binding protein composed of a peptide chain and a heme prosthetic group. It is mainly distributed in cardiac and skeletal muscle tissues, and has the function of transporting and storing oxygen in muscle cells. When the myocardium is damaged, it diffuses out of the myocardium and enters the blood circulation.

Myo protein is a small molecular substance, which is the earliest abnormally increased cardiac protein marker after myocardial injury. In acute myocardial infarction (AMI), the serum myoglobin concentration deviates from the normal value within 2 to 3 hours in the initial stage of chest pain, reaches the highest value in 6 to 9 hours, and returns to normal in about 24 hours.

Therefore, within 1.5 to 6 hours after the occurrence of AMI, the dynamic detection of serum myoglobin level can early diagnose whether AMI occurs. If the second detection value is significantly higher than the first detection value, it has extremely high positive predictive value; if there is no difference between the two detected values of dynamic detection, it has 100% negative predictive value, excluding the possibility of AMI.

Myo has high sensitivity in the early detection of myocardial injury, but poor specificity. It should be noted that myoglobin may be elevated in severe shock, severe extensive trauma, end-stage renal insufficiency, myocarditis, acute infection, myositis, or myopathy. Therefore, attention should be paid to the differential diagnosis with AMI, and the nature of chest pain cannot be determined based on the Myo single test results.

Today, clinical diagnosis of AMI usually requires combined detection of CKMB, Myo and cTnI. The sensitivity of CKMB is lower than Myo in the early diagnosis of AMI, but the specificity is highe. cTnI has myocardial specificity and is the most reliable marker for the detection of myocardial infarction, but it begins to increase 3-8 hours after chest pain occurs. If cTnI is detected alone, it may not be able to diagnose early AMI patients. Therefore, the dynamic combo test of Myo, cTnI, and CKMB within 6 hours of chest pain is helpful to timely and accurately distinguish AMI patients, thereby creating conditions for timely rescue of patients.

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Clinical Significance of Troponin Ic(2023年06月01日)

SEKBIO — 2023-05-22T15:23:44+0900

https://www.sekbio.com/clinical-significance-of-troponin-ic.html

1. The diagnosis of myocardial injury by ctni troponin

Among the many clinical biochemical indicators for the diagnosis of acute myocardial infarction (AMI), CK-MB was once considered the "gold standard", and has been widely used for many years.

2. Troponin ic as an indicator of thrombolytic therapy after AMI

Intravenous injection of thrombolytic drugs is a commonly used treatment method for AMI in recent years, and judging whether reperfusion occurs after treatment has also become one of the most concerned issues for clinicians.

3. Troponin ic in the diagnosis of perioperative myocardial infarction

The diagnosis of myocardial infarction after coronary artery bypass grafting plays an important role in cardiac surgery. The cTn is a sensitive and specific marker of perioperative myocardial infarction, which can identify minor perioperative myocardial injury that does not meet the criteria of conventional perioperative myocardial infarction.

4. Troponin ic for the diagnosis of myocarditis

Compared with CK activity, cTnT has higher detection sensitivity in myocarditis due to its relatively high serum detection value and longer rise time, and serum cTnT can be used as a diagnostic marker for acute myocarditis.

5. The relationship between ctni troponin and renal failure

Ischemic heart disease is one of the leading causes of morbidity and mortality in patients with end-stage renal disease, accounting for approximately 40% of total mortality; approximately 25% of these ischemic heart diseases develop AMI. Therefore, in the clinical treatment of patients with advanced renal disease, the diagnosis of cardiovascular complications has become a crucial issue. There are differences in the detection values of cTnT and cTnI in the serum of patients with advanced kidney disease.

6. Differential diagnosis of skeletal muscle injury by ctni troponin

Cardiac and skeletal muscle cells are closely related. During the embryonic period, the two muscles shared many similarities in gene expression, but the expression was different during terminal differentiation. Therefore, the specificity of myocardial injury marker detection in patients with skeletal muscle injury is a matter of concern.

As the second generation cTnT assay has ruled out the cross-reactivity of the assay, cTn can be used as a better marker for the diagnosis of myocardial injury in patients with skeletal muscle injury.

7. Diagnosis of myocardial injury in hypothyroid patients by ctni troponin

Hypothyroidism leads to elevated cholesterol, predisposing patients to coronary artery disease and AMI. At the same time, patients with hypothyroidism often have symptoms of skeletal muscle damage such as cramps and myalgia. Therefore, the serum CK and CK-MB of this kind of patients all increased to varying degrees. At this time, cTn is a better marker for the diagnosis of myocardial injury in hypothyroid patients.

8. Observation of the drug effects by ctni troponin

The cTn is also used to observe the relationship between the pharmacological effects of certain drugs and the heart, to understand whether to improve or exacerbate myocardial ischemia.

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What is Squamous Cell Carcinoma Antigen?(2023年02月24日)

SEKBIO — 2023-02-24T16:29:27+0900

https://www.sekbio.com/products/triiodothyronine-t3-antibody/

With the accelerated pace of modern life, more and more people feel that their bodies are getting worse every day, even young people have this idea. Do you know what squamous cell carcinoma antigen is? Let's take a look at the introduction together.

Ⅰ. Introduction of squamous cell carcinoma antigen (SCC)
Squamous cell carcinoma antigen (SCC) is a tumor marker with good specificity and the earliest used to diagnose squamous cell carcinoma. It was originally isolated from cervical cancer tissue and belongs to the serine protease inhibitor family in terms of biological activity, and it includes two genes SCC1 and SCC2. SCC inhibits apoptosis and participates in the differentiation of the squamous epithelial layer in normal squamous epithelial cells, participates in tumor growth in tumor cells, and contributes to the diagnosis and monitoring of all squamous cell-derived cancers.

Ⅱ. Clinical application of squamous cell carcinoma antigen (SCC)
SCC is mainly used in the diagnosis of squamous cell carcinoma, including squamous cell carcinoma of the cervix, head and neck, esophagus, lung and trachea, genitourinary tract, and anal canal, as well as prognosis, disease monitoring, and efficacy evaluation.

1. It has a high diagnostic value for cervical cancer: the sensitivity of primary cervical squamous cell carcinoma is 44%-69%; the sensitivity of recurrent cancer is 67%-100%, and the specificity is 90%-96%; the determination of serum SCC has an important prognostic role, and the level of serum SCC in patients before treatment is the best independent predictive marker other than lymph node status.

The SCC concentration decreases significantly after radical resection of cervical cancer; it can prompt recurrence early, and 50% of patients have an increase in SCC concentration 2-5 months before clinical diagnosis of recurrence, and it can be used as an independent risk factor. SCC detection is a good method for monitoring disease recurrence in patients with cervical cancer after radiotherapy and chemotherapy.

2. Auxiliary diagnosis of skin cancer: The serum SCCA level will increase in patients with skin squamous cell carcinoma, especially in the advanced stage. For some primary skin squamous cell carcinoma, the clinical severity is related to the serum SCCA level. It can be used as an early warning indicator of skin cancer recurrence risk.

3. Auxiliary diagnosis of head and neck tumors: Serum SCCA levels in patients with head and neck tumors are elevated, and their levels are related to disease stages. Serum SCC level before treatment has prognostic significance for patients with locoregional tumors. SCCA is a good marker for monitoring residual lesions after the first treatment and can guide other adjuvant treatments.

4. Lung cancer: The prevalence of tracheal tumors is low, and the diagnostic accuracy is not high. The currently available markers are not suitable for screening asymptomatic people. Tumor markers must be compared with standard clinical methods (eg, imaging, cytopathology) at the time of diagnosis, combined with CEA for lung adenocarcinoma, combined with NSE for small cell lung cancer, and combined with CYFRA211 to improve the sensitivity of lung squamous cell carcinoma.

5. Prediction of esophageal squamous cell carcinoma: the positive rate increases with the development of the disease, and the combination of SCC and CYFRA21-1 can improve the detection sensitivity.

6.Diagnosis and monitoring of other squamous cell carcinomas: vulvar cancer, bladder cancer, anal canal cancer, penile metastatic squamous cell carcinoma, laryngeal squamous cell carcinoma, etc.

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What to Do if the Squamous Cell Carcinoma Antigen is Elevated?(2023年02月24日)

SEKBIO — 2023-02-24T16:28:56+0900

https://www.sekbio.com/products/thyroid-stimulating-hormone-receptor-tshr-antibody/

Sometimes during physical examination, one or two of the tumor marker are found to be elevated by chance, such as squamous cell carcinoma antigen, then people find it hard to sleep at night, and don't know what to do.

Ⅰ. Scope of application of squamous cell carcinoma antigen (SCC)
Squamous cell carcinoma antigen (SCC) was first used to diagnose squamous cell carcinoma. It is a glycoprotein, abbreviated as SCC, which exists in the plasma of uterine, lung, head, and neck squamous epithelial cancer cells, and is more abundant in non-keratinizing cancer cells.

Squamous cell carcinoma antigen (SCC) is mainly used for cancer prevention screening and is also commonly used to monitor tumor treatment effects, such as recurrence, metastasis, and prognosis. However, the increase in SCC is not necessarily a tumor, so when the increase is found, a systematic examination and regular review should be carried out. If no tumor is found, there is no need to panic.

Ⅱ. Elevated squamous cell carcinoma antigen (SCC) can be seen in:

1. Cervical cancer and other gynecological tumors have a sensitivity of 44%-69% to primary cervical squamous cell carcinoma. It plays a certain role in early diagnosis and disease monitoring, and the concentration decreases significantly after cervical cancer surgery;

2. Lung squamous cell carcinoma, combined detection of CYFRA21-1, CEA, NSE, has certain significance for lung tumors;

3. For esophageal squamous cell carcinoma, the combined detection of CYFRA21-1 has higher sensitivity;

4. Other squamous cell carcinomas: head and neck cancer, vulvar cancer, bladder cancer, anal canal cancer, skin cancer, etc.

5. Non-tumor diseases, such as liver cirrhosis, hepatitis, and renal failure, tuberculosis, pancreatitis, pneumonia, psoriasis, etc.

6. If the blood sample is contaminated with sweat, saliva, or other body fluids, it will cause false elevations.

Elevated SCC must be vigilant. First of all, the patient will be re-examined after 1-2 weeks. If it is still high, CT of the chest, abdomen, and pelvis should be checked and enhanced. If possible, a PET-CT examination can be performed. If SCC continues to rise, there are often lesions, so we must be vigilant. Early detection can be treated early, and the effect will be better.

Although squamous cell carcinoma antigen is a kind of tumor index, it does not mean that the tumor is elevated. For patients with elevated levels, analyze the reasons based on the specific situation. For patients with significantly elevated levels, it is generally necessary to improve the relevant chest or pelvic CT and cytology examinations.

We should treat the increase of tumor markers correctly, and don't panic day and night because of the increase of tumor markers. If no tumor lesions are found after systematic examination, regular review is enough.

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Chinese IVD Raw Material Market is Growing Rapidly and Enterprises Are Favored by Capital(2023年02月24日)

SEKBIO — 2023-02-24T16:28:28+0900

https://www.sekbio.com/products/thyroid-peroxidase-tpo-antibody/

China's IVD industry has developed rapidly in recent years, and China's market capacity has reached the level of 100 billion yuan. According to the data, the scale of China's IVD market has increased from 42.75 billion yuan in 2015 to 80.57 billion yuan in 2019, with a compound annual growth rate of 17.2%. It is estimated that by 2030, the scale of China's IVD market will reach 288.15 billion yuan, becoming the world's largest IVD antibody product consumer country.

1. The rapid development of the Chinese market for IVD raw materials
IVD raw materials, that is, materials used to prepare IVD products, mainly include antigens and antibodies, enzymes and coenzymes, probes, primers, microspheres, etc. The quality of raw materials has a significant impact on the performance and detection accuracy of IVD products, so the upstream links have a strong voice in the IVD industry chain.

However, the production of IVD raw materials has long been a bottleneck in China's IVD industry. The demand for IVD raw materials continues to grow, but the core technology has not progressed to the same extent. Due to the great difficulty in research and development of IVD raw materials, there is still a gap between China's domestic IVD raw materials and imported raw materials in terms of production technology, purity, and quality.

As China's IVD raw material companies invest a lot of money in basic research and development, their R&D innovation capabilities and raw material process quality continue to improve.

Currently, the overseas new crown pneumonia epidemic situation is still relatively severe, and the import cycle of IVD raw materials has become longer. Chinese IVD manufacturers gradually realized the importance of mastering the core industrial chain and started to reserve resources of domestic suppliers to establish a more secure supply chain step by step. In addition, many IVD manufacturers in China have begun to penetrate upstream of the industrial chain by acquiring or establishing raw material companies to solve the problem of raw material supply.

2. The development scale of IVD raw material enterprises
In recent years, IVD raw material companies have also been favored by capital. China's IVD raw material industry took more than ten years to develop from a niche market with little money to a scale of 10 billion yuan.

According to data released by a research institute, the market size of China's IVD raw materials field has increased from 3.3 billion yuan in 2015 to 8.2 billion yuan in 2019, with a compound annual growth rate of 25.8%. It is estimated that by 2024, its market size will reach 20 billion yuan.

Although imported IVD raw materials still dominate the market from the market structure perspective. With the improvement of the technical level of Chinese enterprises and the emphasis on the localization of the industrial supply chain, China's IVD raw material industry is developing rapidly, and it is expected to realize import substitution gradually. According to the released data, from 2015 to 2019, the compound annual growth rate of China's domestic IVD raw material market scale was 30.6%, higher than the growth rate of imported products.

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Market Space Analysis of IVD Raw Materials(2023年02月24日)

SEKBIO — 2023-02-24T16:27:50+0900

https://www.sekbio.com/products/thyroid-function-antibody/

IVD raw materials are upstream of the entire industry chain, and their quality determines the quality of in vitro diagnostic reagents and the accuracy of the final diagnosis results, which is one of the core links of the industry chain.

1. Overview of IVD raw materials
In a broad sense, IVD raw materials include biologically active/non-biologically active materials used to prepare in vitro diagnostic reagents and components required to develop in vitro diagnostic equipment. In a narrow sense, IVD raw materials refer to the reaction systems used for biochemical, immunological, or molecular diagnostic reagents. Raw materials include four parts: the core reaction system, the signal system, the reaction system carrier, and the reaction environment. The core reaction system raw materials such as antigens, antibodies, and diagnostic enzymes play an essential role in the quality of in vitro diagnostic reagents. They are the key to in vitro diagnostic reagents.

2. The market space for IVD raw materials
Benefiting from the increase in diagnostic testing and the discovery of new markers, the global market for in vitro diagnostic reagent raw materials has maintained steady growth in recent years. In 2019, the global in-vitro diagnostic reagent raw material market was expected to be approximately RMB 30.33 billion. It is estimated that by 2030, the global in-vitro diagnostic reagent raw material market scale will reach about RMB 56.53 billion.

The global in-vitro diagnostic reagent raw material market is prominently characterized by many participants, a small individual scale, and a highly fragmented industry. In contrast, many small and medium-sized laboratories occupy a place in the industry by supplying several characteristic projects.

With China's in vitro diagnostic industry's rapid development, the upstream raw materials market has rapidly expanded. According to the report, China's IVD raw materials market has grown from 3.3 billion in 2015 to 8.2 billion in 2019, with a compound annual growth rate of 25.8%. The market size in 2024 will reach 20 billion yuan, and the compound annual growth rate from 2019 to 2024 will be 19.4%.

From the perspective of market competition, imported products still occupy the dominant position in the IVD raw material market. In 2019, the market size of imported products was 7.3 billion yuan, accounting for 88% of the IVD raw material market. The market size of Chinese products was 1 billion yuan, accounting for a small proportion, but the development speed was fast. The compound growth rate from 2015 to 2019 reached 30.6%, higher than that of imported products.

With the continuous improvement of the technical level of Chinese enterprises and the increasing emphasis on the localization of the biotechnology industry supply chain, the market size of domestic in vitro diagnostic reagent raw materials is expected to grow at a compound annual growth rate of 23.3%. It will reach 2.7 billion yuan by 2024 and gradually realizes import substitution.

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What Does Cancer Antigen 125 Mean?(2023年02月24日)

SEKBIO — 2023-02-24T16:27:23+0900

https://www.sekbio.com/products/tumor-marker/

Cancer antigen 125 is abbreviated as CA125, which belongs to carbohydrate antigen, and its clinical reference value of <35,000 U/L is considered normal. It also exists in fetal coelom epithelial secretions, amniotic fluid, the fallopian tube, uterus, and endocervix in adults, seen in the first three months of pregnancy, or other diseases. It is more common in ovarian-related cancer, but the increase is not necessarily a tumor.

1. Cancer antigen 125 can diagnose ovarian cancer
Ovarian cancer has specific clinical value, especially for observing the curative effect and judging recurrence. The serum cancer antigen 125 of patients with ovarian epithelial cancer is significantly increased, reaching several times the average value. The sensitivity of early diagnosis can be 50%-90%. At the same time, cancer antigen 125 mainly exists in ovarian cancer tissue cells.

2. Cancer antigen 125 can judge whether the tumor is benign or malignant
3%-6% of benign ovarian and uterine tumors can also significantly increase cancer antigen 125. Still, it is usually limited to less than 100,000 U/L, common in ovarian epithelial tumors, teratoma, uterine fibroids, and so on.

3. Cancer antigen 125 can identify the nature of the mass
Cancer antigen 125 can also identify pelvic tumors and masses, especially for postmenopausal women, to determine ovarian masses and cancer. Cervical cancer, breast cancer, gastric cancer, biliary tract cancer, pancreatic cancer, colon cancer, and other gastrointestinal malignancies also have specific positive reactions.

4.Other uses of cancer antigen 125
It is known that the cancer antigen 125 is significantly increased in decompensated liver cirrhosis, liver cancer, and other diseases, but the increase is usually less than 100,000 U/L. Mild elevations of cancer antigen 125 may also occur in acute and chronic pancreatitis, benign gastrointestinal illness, endometriosis, other diseases, or pregnancy.

In addition, it is recommended that patients be combined with other laboratory tests, CT, ultrasound, and other imaging tests to confirm the disease, and a tumor biopsy should be performed if necessary. At the same time, maintain a good attitude to face the condition, and cooperate with doctors to actively treat and prevent a recurrence.

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What Are Cardiac Markers?(2023年02月24日)

SEKBIO — 2023-02-24T16:26:56+0900

https://www.sekbio.com/products/thyroglobulin-tg-blood-test-antibody/

With the deepening of scientific research and the advancement of technical methods, cardiac markers have undergone more than 60 years of development, from the earliest glutamate transferase to the current troponin and N-terminal brain natriuretic peptide precursor, forming a complete and mature testing system.

1. What are cardiac marker in blood tests?
The first thing to understand is what are cardiac markers? Cardiac markers are biochemical indicators that reflect heart disease and are helpful for the early diagnosis, clinical analysis, and prognosis evaluation of the myocardial injury, especially acute myocardial infarction in coronary heart disease.
Cardiac diseases mainly include myocardial disease, heart valve disease, pericardial disease, coronary artery disease and other cardiovascular diseases, arrhythmia, heart failure, etc.
In addition to the medical history, symptoms, and signs, the diagnosis of heart disease requires the support of auxiliary examinations, such as electrocardiogram, laboratory tests, echocardiography, chest X-ray, CT, and other means.

2. The normal range of cardiac markers
The normal ranges of the three myocardial markers are different for different medical institutions and testing methods. In most hospitals, troponin <0.02ng/mL, myoglobin <70ng/mL, and creatine kinase isoenzyme <25U/mL in the three myocardial infarction items in most hospitals. L. However, when it comes to individual test results, there is a normal range on the test sheet, because different machines, reagents, and detection methods will be different. It cannot be simply compared directly. You should refer to the normal range of the specific test sheet.
The three indicators of myocardial markers are mainly used to identify whether there is myocardial infarction, myocarditis, and myocardial injury. The time difference between the rise and fall of the indicators in the blood, helps to judge the onset time of myocardial infarction. For example, 30 minutes after myocardial infarction, myoglobin can be detected in the blood, and creatine kinase isoenzyme and troponin can be detected 3-6 hours later.

3. Time of cardiac markers
Time of myocardial infarction markers: Serum troponin starts to increase 3 to 4 hours after acute myocardial infarction, reaches a peak in 2 to 5 days, and lasts for 10 to 14 days. Other cardiac markers include creatine kinase and its isoenzymes, aspartate aminotransferase, lactate dehydrogenase, and its isoenzymes. Creatine phosphokinase isoenzyme and creatine phosphokinase increased within 4 to 6 hours after the onset, peaked at 16 to 24 hours, and returned to normal within 3 to 4 days. Myoglobin increased 2 hours after onset, peaked at 12 hours, and returned to normal at 24 hours.

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