The washing solution of the strong bacteria must be washed

The pH value can be read directly on the instrument(2020年06月15日)

centrifugetubeae — 2020-06-15T15:33:21+0900

Welcome to freezing cryo tube and leave relevant information, our professional engineers will contact you as soon as possible!.4 beaker, 50mL 4. Steps for measuring the pH value of common makeup water in the market: This experiment uses common makeup water in the market as a sample,

a high-precision ST5000 pH meter, and an easy-to-use composite glass electrode STMICRO5 (a combination of a pH glass electrode and a reference electrode) as a pH measuring instrument.3 Sample measurement Wash the electrode with water, dry it with filter paper, insert the electrode into the sample to be tested, start the stirrer, wait for the acidity meter reading to stabilize for 1 minute, stop the stirrer, and read the pH value directly from the instrument. . iv)

After removing the electrode from the first calibration solution and washing it with deionized water, wipe it and place it in the second calibration solution, click 'Next', wait for the value to stabilize for about 30s, and complete the calibration operation of the second pH point.2. The pH value can be read directly on the instrument. 'Cosmetics Safety Technical Specification' Application scope of pH measurement method This method specifies the pH value of cosmetics with a pH meter. This method is suitable for measuring the pH value of cosmetics. (STMICRO5 electrode application picture) ST5000pH meter calibration steps: i) Turn on the power and light up the meter screen.

This solution has a pH of 4. upset. The rapid development of the cosmetics industry, while the use of cosmetics is becoming more and more widespread, its safety incidents also frequently occur.1 Precision acidity meter (accuracy 0.5 balance As a laboratory equipment supplier with a long history of 111 years, Ohaus can provide users with Provide high-precision pH meter, electrode and stable and reliable Tianping products. The meter has no buttons and is intuitive and convenient to operate.55g of disodium hydrogen phosphate (Na2HPO4) at 105 ° C for 2h, and dissolve in water.3 Sodium borate standard buffer solution: Weigh 3.18 to perform three-point calibration on the ST5000 meter.
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The company has a human-sourced spot plasmid library(2020年06月09日)

centrifugetubeae — 2020-06-09T10:48:44+0900

Choosing Laboratory Consumables of different concentrations can separate DNA molecules of different size ranges. Note: Weizhen Biological is committed to providing high-quality virus packaging services for scientific researchers. Experimental principle Agarose gel electrophoresis is often used to separate and identify mixtures of DNA and RNA molecules. Ethidium bromide (EB) used in electrophoresis is moderately toxic and highly carcinogenic. TAE is inexpensive but has low cushioning capacity., but the overall effect is similar to traditional EB. Service items include: scientific and clinical grade adenovirus, slow Virus, adeno-associated virus (AAV) packaging, plasmid vector construction, TALEN gene knockout, gene mutation, etc.

So far, the company has a human-sourced spot plasmid library (18 000), an adenovirus spot library (12 000), and an adeno-associated virus (AAV) spot library. For large DNA strand electrophoresis, the temperature should be <15 ° C. The greater the electric field strength, the faster the movement of the charged particles. For large-scale electrophoresis, you can choose to run at 0. When the EB is added in advance, the speed of DNA movement may be reduced by about 15%, and the effect of different configurations of DNA is different.

Analysis of common problems Common problems Cause countermeasures DNA bands fuzzy DNA degradation experiment process to avoid nuclease contamination of the electrophoresis buffer Old Electrophoresis Buffer After repeated use, the ionic strength decreases, the pH value rises, and the buffering capacity weakens, which affects the electrophoresis effect. 2. EB is easily degraded under light.5-1. The countermeasures are: adjust the PCR system and PCR program, and find out the appropriate conditions. The electrophoretic conditions used are not suitable.

Therefore, comprehensively consider the use of TBE buffer. Dilute the DNA DNA chain with 20mM Nacl buffer solution. According to the size range of the separated DNA molecules, select a gel with an appropriate ratio, weigh an appropriate amount of agar powder, put it in a conical flask, and add an appropriate amount of TBE electrophoresis buffer. Irregular DNA band migration electrophoresis conditions are not suitable when the electrophoresis voltage is not more than 6V / CM, the temperature is <30 ° C, and the large DNA strand is electrophoresed, the temperature should be <15 ° C.
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Resuspend cells by adding an appropriate amount of pre-chilled PBS(2020年05月28日)

centrifugetubeae — 2020-05-28T12:06:32+0900

2. Inhale the cell culture supernatant into a centrifuge tube and centrifuge at 1000 × g for 20 minutes to remove cell debris and impurities. Carefully collect the supernatant (plasma). Tissue homogenate 1) Rinse the tissue with pre-chilled PBS (0. 5) Centrifuge at 10,000 × g for 10 minutes at 4 ° C to remove cell debris. Use a pyrogen-free and endotoxin-free test tube or centrifuge tube to collect blood samples, and place the test tube or centrifuge tube at room temperature for 2 hours or 4 ° C overnight to isolate the serum. So that the serum can be more separated).

In this case, non-specific color reaction will occur in ELISA experiments, resulting in inaccurate test results. Repeat the freeze-thaw process several times until the cells are completely lysed.. Pretreatment methods are different for different sample types. Proper sample preparation is the first step to ensure the correctness and accuracy of ELISA experiments. 6.

Samples should avoid hemolysis or hyperlipidemia. In a 6-well culture plate, 150-250 μL of PBS is required for each well to resuspend the pipette tips suppliers cells. Plasma Use blood collection tubes or centrifuge tubes containing anticoagulants to collect blood samples. 3. Hemolysis should be avoided during the collection of blood samples, because red blood cells will release substances with peroxidase activity when they are lysed. Centrifuge at 1000 × g for 15 minutes at 4 ° C for 30 minutes after collection. 3)

Resuspend cells by adding an appropriate amount of pre-chilled PBS (protease inhibitors are recommended immediately before use). Please read the instructions of the ELISA kit carefully before testing to see if the kit has special requirements for anticoagulants. Centrifuge at 1000 × g for 20 minutes at 4 ° C, carefully collect the supernatant (serum), and immediately perform the measurement. It is recommended to store the collected serum in aliquots at -20 ° C or -80 ° C to avoid repeated freeze-thaw cycles.01M, pH 7. The suspension can also be sonicated using an ultrasonic cell disruptor to lyse the cells. Cell culture supernatant. 4. 3)
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The washing solution of the strong bacteria must be washed(2020年05月21日)

centrifugetubeae — 2020-05-21T12:11:22+0900

The 5ml Pipette tips medium needs to be clarified to facilitate the observation of the growth of bacteria. ④ Close the exhaust valve to increase the vapor pressure to the specified requirements and maintain it for the specified time (depending on the nature of the sterilized article and related circumstances). 3. 8. When inoculating food samples, special work clothes, caps and slippers must be worn. The door should be closed tightly before and after use in the sterile room, and the UV lamp should be turned on. If there is damage or the tampon is removed, it cannot be used as a sterile item.

(3) The bottle and bagged food should be opened with sterilization operation, and the above method should be selected for inoculation after treatment. At present, there are many kinds of dry culture media. 3. Some substances are easily destroyed by high-pressure steam sterilization. (2) Check whether the pressure gauge stays at zero when the steam is exhausted, close the door and cover, and then observe the use of the pressure cooker or vertical pressure steam sterilizer after the steam or heat is applied: ① Portable Add 3L of water to the main body of the pressure cooker, and add 16L of water to the vertical pressure cooker (the water should be replenished when it is reused, and the water becomes turbid and needs to be replaced).

A small window of 0. (2) Intermittent sterilization method 1. (8) After the completion of each batch of sterilization, record the name, quantity, temperature, time, and operator of the sterilization. Fifth, the preparation of culture media requires that the quality of culture media preparation will directly affect the growth of microorganisms.

The sterilization of the culture medium must not only achieve the purpose of complete sterilization, but also pay attention not to reduce its nutritional value due to heating. (2) Close the drain, open the intake valve, and disinfect for 10-20 minutes as required. VII. (2) Temperature and time of pressure steam sterilization. 2. If the indoor hanging UV lamp is used for disinfection, a 30W UV lamp is needed, the distance is 1. Weigh out the ingredients according to the formula of the culture medium, and then dissolve them in distilled water. The washing solution of the strong bacteria must be washed in the beaker. ②
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