Chinese Peptide Company

Multiple Disulfide Bridges(2024年02月07日)

chinesepeptideco — 2024-01-29T14:41:48+0900

https://www.chinesepeptideco.com/multiple-disulfide-bridges.html

what is a disulfide bridge

Disulfide linkages, also known as disulfide bridge, are reversible covalent connections that can form between two side chain thiol groups and can occur between cysteine (Cys) residues in proteins and peptides. Disulfide bonds give linear peptide sequences structural limitations that increase molecular stiffness and more stable secondary structures. Peptides with a high disulfide content are less likely to be broken down by enzymes and can occasionally boost binding affinity to the relevant receptor.

Disulfide-rich peptides that are conformationally stable have given rise to numerous powerful and selective peptide classes. The host-defense peptides (such as -defensins and -defensins) and poison peptides from scorpions, cone snails, snakes, and spiders are significant classes with a variety of therapeutic applications. Conotoxin and chlorotoxin are strong ion channel blockers that have been proposed as cancer and chronic pain therapies.

For more information about chinese peptide, please feel free to contact us!

Macrocyclization(2024年02月06日)

chinesepeptideco — 2024-01-29T14:41:20+0900

https://www.chinesepeptideco.com/macrocyclization.html

All forms of cellular processing depend heavily on interactions between proteins and proteins and peptides. Proteins and peptides are each other's natural companions, and because peptides may adapt to the frequently changeable protein surface, they attach to proteins with high affinity when used as ligands. Due to their protein-like nature, peptides are biocompatible; but, as medication candidates, they have a number of disadvantages, such as limited plasma bioavailability, instability from proteolytic enzymes, and poor passive membrane permeability. With linear peptides, especially those that preserve -helical secondary structures, some success has been attained. By using conventional "peptide-stapling" techniques, these motifs can be added to stable -helical motifs, however stapled peptides may have low bioactivity and poor solubility. The modification of peptides through macrocyclization has been one method to increase macrocyclic peptide stability.

Macrocyclization Chemistry
CPC Scientific has the know-how to execute a range of ring-closure procedures and specializes in the synthesis of complicated peptide macrocyclic. We regularly create peptide macrocycles with the following types of bonds:

Disulfide bridges that are numerous and site-specific (Cys-Cys, Pen-Cys, and Pen-Pen)

Cyclizations of amide bonds (lactam)

Sidechain-to-sidechain, sidechain-to-tail, head-to-sidechain, and head-to-sidechain

Hydrocarbon-stapled peptides are connected by backbone-to-backbone, backbone-to-sidechain, backbone-to-head, and backbone-to-tail thioether bridges.

Azide-alkyne cycloaddition catalyzed by copper (Click Chemistry)

Macrocycle Types 3 Set

X = S-S (disulfide bridge)

X = NH-CO (lactam bridge, amide bond)

X = S (thioether bridge, sulfide bridge)

X = O-CO (lactone bridge, depsipeptide)

X = CH=CH (alkene bridge, hydrocarbon stapled)

Y = NH (lactam bridge, amide bond)

Y = O (depsipeptide)
Cyclized RGD Motif
More attention is being paid to localization and cellular absorption as research into medication delivery platforms progresses. Targeting cancer cells that overexpress specific receptor proteins that may recognize and internalize CTPs through receptor-mediated endocytosis has become possible thanks to the development of cell-targeting peptides (CTPs). Cell adhesion receptors control the process of angiogenesis, which is reliant on vascular endothelial cell migration and infiltration in tumor spread. Integrins are a significant group of heterodimeric transmembrane proteins that are essential for cell adhesion, cell signaling, and apoptosis[1]. The v3 integrins are the members of the integrin family that have undergone the most research because of their significance in tumor angiogenesis and metastasis. V3 integrins are an appealing target for cancer therapies (e.g., radiotracers, cancer medicines), as they are overexpressed in a variety of tumor cell types (such as breast, prostate, and ovarian malignancies) but missing in healthy tissue types. By inhibiting angiogenesis, inhibition of v3 integrin receptors has been linked to tumor prevention and decreased tumor growth. It has been created and synthesized to make antagonists based on peptides that bind to v3 integrins. Kessler and colleagues created cyclo[Arg-Gly-Asp-D-Phe-Val] (c[RGDfV]), one of the most effective and selective of these peptide antagonists.[2]

Numerous RGD motif peptides have been created by CPC Scientific and are intended for use in multivalent molecular structures and as conjugates with chelating moieties (such as DOTA, NOTA, etc.). For therapeutic usage, cyclic RGD (cRGD) peptide-based nanomedicines have been created. A brain glioma cascade delivery method using dual targeting liposomal systems made up of cRGD and transferrin (TF) mixed with a liposome (cRGD/TF-LP) has been established. The finding that cRGD peptide coupled with TF permits distribution across the blood-brain barrier (BBB), permitting RGD-targeting in the brain, was crucial to this approach. When paired with paclitaxel, cRGD/TF-LP creates a novel system that can specifically target brain-based gliomas, which are challenging to treat with chemotherapeutic drugs alone.[3]

cRGD Nanomedicine
Mesoporous silica nanoparticles (MSNs), a platform that has mostly been researched for controlled drug release, are another nanoparticle (NP)-based cRGD targeting method. MDA-MB 435 metastatic breast cancer cell lines have been successfully targeted and subjected to apoptosis using MSN-cRGD loaded with camptothecin (CPT). It was shown that this cell line had increased localisation and cellular absorption using the MSN-cRGD platform in combination with a fluorescent tag.[4]

CPC Peptideguide Macrocycles Mda Mb 435 Cell

Figure. MDA-MB 435 cell line with 20ug/mL (left) NP (control), (middle) cRGD-NP overlay images showing cellular membrane (red) and cell nucleus (blue), and (right) cRGD-NP dye (green).
Rgd Thiol Surface

Figure. Nanoparticle (NP)-based cRGD targeting system is mesoporous silica nanoparticles (MSNs).[3]

References

Van der Flier, Arjan, and Arnoud Sonnenberg. “Function and interactions of integrins.” Cell and tissue research 305, no. 3 (2001): 285-298.

Wermuth, J., S. L. Goodman, A. Jonczyk, and H. Kessler. “Stereoisomerism and biological activity of the selective and superactive αvβ3 integrin inhibitor cyclo (-RGDfV-) and its retro-inverso peptide.” Journal of the American Chemical Society 119, no. 6 (1997): 1328-1335.

Ferris, Daniel P., Jie Lu, Chris Gothard, Rolando Yanes, Courtney R. Thomas, John‐Carl Olsen, J. Fraser Stoddart, Fuyuhiko Tamanoi, and Jeffrey I. Zink. “Synthesis of biomolecule‐modified mesoporous silica nanoparticles for targeted hydrophobic drug delivery to cancer cells.” Small 7, no. 13 (2011): 1816-1826.

For more information about chinese peptide, please feel free to contact us!

Isotope Labeling(2024年02月05日)

chinesepeptideco — 2024-01-29T14:40:45+0900

https://www.chinesepeptideco.com/isotope-labeling.html

About 30,000 human genes are known to encode more than a million proteins, according to genomic research. Protein quantification, structural analysis, localization investigations, and post-translational modification detection are all parts of the field of proteomics, which is the study of proteins encoded by the genome. In proteomics, mass spectroscopy-based methods have become an effective tool. Regarding retention time, ionization effectiveness, and fragmentation mechanisms, stable isotope labeled peptides (SIL peptides) are structurally and chemically identical to their endogenous counterparts. Therefore, both internal standards and template analytes are excellent choices. Incorporating amino acids having the required isotopes, such as deuterium (D), 13C, 15N, or 18O into the peptide during synthesis allows for the labeling of peptides with one or more hydrogen, carbon, nitrogen, or oxygen isotopes.

Utilizing SIL peptides as internal standards, the Absolute Quantification technique (AQUA) provides focused quantification of protein and post-translational changes in complicated protein mixtures. In a biological sample, the SIL peptide is added during or following protease digestion. Selective reaction monitoring (SRM) in a mass spectrometer is used to identify the heavy SIL peptide and the light endogenous peptide fragment that it is composed of. The quantity of endogenous protein can be determined using the known addition of radioisotope labelling and the intensity ratio of the two peptides.

Isotopic Amino Acid Table
AMINO ACIDISOTOPEMASS DIFFERENCEISOTOPIC ENRICHMENT
Alanine13C3, 15N4>99%
Arginine13C6, 15N410>99%
Isoleucine13C6, 15N7>99%
Leucine13C6, 15N7>99%
Lysine13C6, 15N28>99%
Phenylalanine13C9, 15N10>99%
Proline13C5, 15N6>99%
Valine13C5, 15N6>99%

SIL Peptides in Structural Analysis
A potent method for examining the kinetics, structural details, and molecular interactions of biomolecules is nuclear magnetic resonance (NMR). The rate at which biomolecules relax as they separate from the target to which they are attached can be measured using this method. For NMR analyses of proteins, peptides labeled with D (spin of 1), 13C (spin 1/2), and 15N (spin 1/2) are appropriate. The mechanism of protein splicing, a post-translational autocatalytic process in which an intervening sequence, known as an intein, is excised from a host protein, the extein, is studied using a combination of segmental isotopic labeling and NMR spectroscopy.

High Quality SIL Peptides from CPC
High-quality peptides enriched with stable isotopes are necessary for structural studies and absolute quantification of a complex protein mixture at very low concentrations. Superior quality and high isotopic enrichment are promises made by the CPC Scientific SIL Peptide Custom Synthesis Service. In our cutting-edge peptide lab, these stable isotopic peptides are created using the most recent Fmoc solid-phase peptide technology. To determine the final purity and guarantee that our customers only receive the best peptides for absolute quantification and other investigations, all heavy isotope labeled peptide go through rigorous analytical HPLC and mass spectrometric examination.

Peptides include two significant isotopes, 15N and 13C, in a certain order of amino acids. Each atom in the peptide can be found in several places and includes over 99% of an enriched isotope. For instance, a peptide containing a Leu amino acid that is isotopically tagged with one 15N and six 13C has a molecular weight that is seven units higher than the identical unmarked peptide.

SIL Peptide Applications
Quantitative functional proteomics

Quantification of proteins with post-translational modifications

Study of protein structure

Monitoring of protein expression

Finding of biomarkers

Pharmacokinetics

Metabolomics

Clinical biochemistry for monitoring of drugs and metabolites

testing for doping

Profiling of cell signals and verification of pathways

Analysis of protein cross-links

SIL Peptide Modifications
Phospho-Tyr, Ser, Thr (single or multiple)

Sulfo-Tyr (single or multiple)

Methylated Arg, Lys

Chloro-Tyr

Met-oxidized

Pyroglutamic acid

Utilizing SIL peptides as stable isotopically labeled internal standards, the Absolute Quantification technique (AQUA) provides focused quantification of protein and post-translational changes in complicated protein mixtures. In a biological sample, the SIL peptide is added during or following protease digestion. Selective reaction monitoring (SRM) in a mass spectrometer is used to identify the heavy SIL peptide and the light endogenous peptide fragment that it is composed of. The quantity of endogenous protein can be determined using the known addition of SIL peptide and the intensity ratio of the two peptides.

For more information about isotopic labelling reaction mechanism and radioactive isotope labeling, please feel free to contact us!

There are many peptide vendors, but we are one of the best choices for you.

GMP Services(2024年02月04日)

chinesepeptideco — 2024-01-29T14:40:13+0900

https://www.chinesepeptideco.com/gmp-services/

CPC is a leading peptide CRDMO specializing in CGMP peptide synthesis. With over 20 years of experience in the peptide industry, we have established ourselves as a reputable supplier of high-quality peptides to researchers and pharmaceutical businesses worldwide. Our expertise lies in synthetic GMP peptide manufacturing for early- and late-phase development, as well as commercial manufacture. With a team of experienced peptide professionals and a strong track record in peptide design and large-scale manufacturing spanning over 30 years, CPC is your trusted go-to CRDMO for all your GMP peptide needs.

Why Choose CPC GMP Services?

Manufacturing Expertise
We have been gmp peptide manufacturing for more than 20 years.

Methods of fragment condensation, ligation chemistry, SPPS, and LPPS.

Any of the defensive tactics (Boc, Fmoc, Cbz) may be used.

Utilized are cutting-edge purification and isolation methods.

A solid commercial process is ensured through skill in process development and scale-up.

Large-scale production has been tailored to complex sequences and peptide changes.

01

cGMP Compliance
The gmp document services program at CPC Scientific complies with FDA/EU cGMP regulations to the letter.

ICH Q7 (International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use) and Code of Federal Regulations Part 210/211 (21 CFR 210/211) Good Manufacturing Practice Guide for Active Pharmaceutical Ingredients, ICH Harmonized Tripartite Guideline Q7)

02

Regulatory Support
Full support for your international and US IND and NDA filings.

All necessary governmental backing for your peptide APIs.

Consultation on phase-appropriate control systems

Information required for the compilation of the Chemistry, Manufacturing, and Controls (CMC) section of the New Drug Application (NDA) and Investigatory New Drug (IND) submissions

Compilation and submission of drug master files (DMF)

Product reviews every year

03

Quick Delivery Time
A key factor in the success of your project is delivery timing. We are able to accept new projects and begin production earlier than many other top peptide producers because of our flexibility and capacity.

04

Manufacturing Capacity
From early research (mg amounts) through commercialization (100 kg), CPC Scientific has the GMP peptide manufacturing capabilities to assist your GMP initiatives. We also have the adaptability, pre-approved workspace, and strong supply chain needed to quickly add new projects to our pipeline of production.

05

Complete Life Cycle Support
At every stage of development, including the discovery phase, preclinical development, clinical trials, and commercial manufacturing, CPC Scientific is by your side.

06

Confidentiality
The primary driver of our business's expansion and success is the delight of our clients. Maintaining the security of trade secrets and sensitive information provided to us by our customers is a crucial component. All private information that we obtain while providing any of our custom services will be completely secret, CPC Scientific guarantees.

07

Proven Track Record
CPC Scientific has more than 20 years of experience working with peptides and a demonstrated track record in process development, bulk-scale manufacture, and bespoke synthesis. With over 100 clinical development projects and 9 commercial products, we have experience in both early- and late-phase projects.

08

US-Based Manufacturing
The United States will be protected from experiencing shortages of essential pharmaceutical supplies by developing robust supply networks. Beginning in 2024, the production facility in Rocklin, California, owned by CPC Scientific, will handle the crucial supply chain requirements for peptide APIs.

For more information about cgmp registered facility and gmp quality control, please feel free to contact us!

There are many gmp companies, but we are one of the best choices for you.

If you are looking for a reliable gmp factory, don't hesitate to contact us!

For more information about polypeptide cdmo, please feel free to contact us!

GMP Production Capacity(2024年02月03日)

chinesepeptideco — 2024-01-29T14:36:32+0900

https://www.chinesepeptideco.com/gmp-production-capacity.html

We have 24 isolated process suites and 19 isolated purification suites, which enable manufacturing capacity of 500 kg/year

CHINA-Based Manufacturing Site

CPC Hangzhou has the largest peptide and oligonucleotide production facility (>750,000 ft² and >500 staff). It develops and manufactures peptide new chemical entities (NCEs), oligonucleotide NCEs, commercial API, research and specialty products (e.g., catalog peptides and cosmetics), as well as offering a range of services and capabilities (e.g., GMP production, project management). There are 24 isolated process suites (19 synthesis & 5 cleavage) and 19 isolated purification suites enable several simultaneous multi-kg batch size projects at the same time.

Equipment Overview
We are always receiving new equipment to enhance our manufacturing capacity. Please contact your local CPC Scientific gmp certified supplements representative for the latest information.

Synthesis Equipment

Large solid- and solution-phase peptide synthesis reactors (up to 1000L capacity)

Hybrid synthesis reactor

Purification Equipment

Preparative HPLC systems with multiple flow rates

Chromatography columns with various diameters (8, 12, 20-in)

Fast protein liquid chromatography (FPLC) systems

Multiple isolated purification suites

Isolation Equipment

Blenders and evaporators

Manifold lyophilizers

Tray lyophilizers (200 L, 500 L)

GMP Manufacturing Equipment.

Enable multi-kilogram Peptide API Manufacturing
3880 Atherton Rd,
Rocklin, CA 95765Peptide API manufacturing
capacity: multi-kilogram41 ,000 square
feet
For more information about gmp production meaning and gmp capacity, please feel free to contact us!

There are many peptide api manufacturers, but we are one of the best choices for you.

GMP Manufacturing(2024年02月02日)

chinesepeptideco — 2024-01-29T14:36:03+0900

https://www.chinesepeptideco.com/gmp-manufacturing/

CPC is a leading peptide CRDMO that focuses on synthetic gmp clinical manufacturing for early and late-phase development, as well as commercial manufacturing. With over 20 years of dedicated service to the peptide industry, CPC is a trusted supplier of top-notch peptides to researchers and pharmaceutical companies worldwide. Backed by the expertise of the CPC Group, who have been actively involved in peptide design and large-scale gmp in manufacturing for over 30 years, your project will be well taken care of.

GMP Peptide Projects by Region

CPC's manufacturing facilities in China comply with the Good Manufacturing Practice (GMP) standards of regulatory agencies such as the Food and Drug Administration (FDA) and the National Medical Products Administration (NMPA)

GLOBAL GMP PROJECTS BY PHASE
CPC services early- and late-phase GMP manufacturing projects and has had over 80 peptides in clinical trials.

As one of the peptide manufacturers, we provide cgmp compliant manufacturing facility, gmp manufacturing process, gmp audit pharmaceutical, cgmp manufacturing facility, gmp auditing and inspection, etc. For more information, please feel free to contact us!

GMP Compliance & Certification(2024年02月01日)

chinesepeptideco — 2024-01-29T14:35:35+0900

https://www.chinesepeptideco.com/gmp-compliance-certification.html

CPC manufactures peptide active pharmaceutical ingredients (APIs) in accordance with FDA/EU cGMP guidelines, which include:

Code of Federal Regulations Part 210/211 (21 CFR 210/211)

ICH Q7 (International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use ICH Harmonised Tripartite Guideline Good Manufacturing Practice Guide for Active Pharmaceutical Ingredients Q7)

Check our audition and Inspection History here:

GMP regulatory compliance History Timeliness

CGMP Compliance
The CPC Quality department is committed to ensuring complete adherence to CGMP standards at every stage of production. Additionally, they rigorously uphold testing and documentation protocols in alignment with the most stringent FDA stipulations.

In-process, release and environmental control testing performed

Standard release testing performed in-house

Audited contract laboratories available for additional testing

Method development and validation, formal method transfer

Stability studies (ICH) and forced degradation.

As one of the gmp certified supplement companies, we provide gmp manufacturing license, good manufacturing process certification, regulatory certification of gmp, fda gmp certified facility, etc. For more information, please feel free to contact us!

There are many peptide manufacturing companies, but we are one of the best choices for you.