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Protease catalyzes peptide bond and hydrolyzes protein into peptone, hydrazone, polypeptide and amino acid. As a kind of alkaline serine protease, keratinase not only has the function of normal alkaline protease, but also can break down disulfide bond and disintegrate keratin.

Specification of Kkeratinase products
NameKeratinaseProduct Series NameNutriGen Keratinase
Activity100000-200000 U/g
StrainParengyodontium albumEC codeEC 3.4.99.11
FormPowder,LiquidCarrierStarch
ColorWhite, Light yellowLoss on drying≤ 10%
Working pH6.0-12.5Optimum pH11
Working T30℃-70℃Optimum T60℃
Particle size80% pass 40 mesh
Package25kg /bag
StorageStored 6 months in dark, cool and dry conditions
CautionAvoid direct sunlight, high temperature

Definition and Dosage of Activity Keratinase
One unit (U) of protease activity is defined as the amount of enzyme which liberates 1 μg tyrosine per minute from substrate casein at 40℃ and pH 10.5 under the conditions of the test.

Recommended dosage is 50-100g/t with 50000 U/g in complete feed.

Functions of Keratinase
1. Supplement the deficiency of endogenous protease for animal.

2. Increase the digestibility of protein and amino acid in poultry and swine feed ingredients.

3. Degrade the trysin inhibitor and lectin, this is what endogenous protease can’t do.

4. Optimized the use of lower digestibility feed ingredient e.g corn, sorghum, feather meal,soybean meal and blood plasma powder.

5. Creat a better environment for animal and decrease pollution from nitrogen for human.

Conclusion of Keratinase
On the basis of these results, it is concluded that the supplement of keratinase into a low CP diet was as effective as normal level in broiler. As shown in diagram 1, keratinase improve the digestibility of dry matter, crude protein, crude fat, general energy and apparent metabolic energy. And in table 4, keratinase has a positive effect on the amino acids digestibility. In this experiment, it is appeared that keratinase improve the growth performance of broiler by increase average diary gain and feed conversion rate.

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