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CHES BUFFER CAS NO.103 47 9

Biological buffer CHES, chemical name 2-cyclohexylaminoethanesulfonic acid, pH buffer range 8.6-10.0, is a zwitterionic buffer used in biochemistry and molecular biology, does not complex with metal ions, CHES buffer is cyclohexane, a member of the amino buffer family.

CHES Buffer Details
CHES buffer is a dipolar ionic (zwitterionic) Good's buffer that can be used in a variety of biological research areas including enzymology, and studies of reactions above the physiological pH range, protein stabilization, mannan Carbohydrase activity assay, DNA digestion and 2'-deoxyadenosine 3'-monophosphate labeling, and as a component of Wilson and Horne extraction buffer.

Desheng CHES Buffer Advantages:
Desheng is the direct manufacturer of CHES buffer, with long-term sufficient stock, fast delivery, stable quality, high purity of 99, good color, no yellowing, and little interference to the biochemical process;

Desheng has a professional CHES R&D team, owning a wide CHES buffer range. Indicators can be customized, and can be packaged according to customer needs. Generally, there are 500g small bottles and 25kg cardboard drums.

The daily output of Desheng CHES buffer reaches 1-2 tons, the production technology and equipment are advanced, and the packaging is rigorous.

Application of CHES Buffer
Used as a buffer for enzymatic reactions;

Samples and running buffers for various capillary electrophoresis;

Shows high affinity to the iodoacetate binding site of liver alcohol dehydrogenase, which can protect the enzyme from being inactivated by iodoacetic acid;

Compared with other buffers, phosphodiesterase has the greatest activity in CHES buffer;

CHES buffer can inhibit the activity of M. luteus at pH 9.5.

How to Prepare CHES Buffer
Weigh 1.036 g of CHES (MW=207.3 g/mol) and add 40 mL of pure water;

The pH value of 40 mL of CHES aqueous solution was measured to be 4.50, and the test temperature was 27 °C;

After adding 1 mL of 1 M NaOH solution, the tested pH value is 8.83;

Add purified water to 50 mL, transfer to a 50 mL sample bottle, and store at room temperature.

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