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Choosing Laboratory Consumables of different concentrations can separate DNA molecules of different size ranges. Note: Weizhen Biological is committed to providing high-quality virus packaging services for scientific researchers. Experimental principle Agarose gel electrophoresis is often used to separate and identify mixtures of DNA and RNA molecules. Ethidium bromide (EB) used in electrophoresis is moderately toxic and highly carcinogenic. TAE is inexpensive but has low cushioning capacity., but the overall effect is similar to traditional EB. Service items include: scientific and clinical grade adenovirus, slow Virus, adeno-associated virus (AAV) packaging, plasmid vector construction, TALEN gene knockout, gene mutation, etc.

So far, the company has a human-sourced spot plasmid library (18 000), an adenovirus spot library (12 000), and an adeno-associated virus (AAV) spot library. For large DNA strand electrophoresis, the temperature should be <15 ° C. The greater the electric field strength, the faster the movement of the charged particles. For large-scale electrophoresis, you can choose to run at 0. When the EB is added in advance, the speed of DNA movement may be reduced by about 15%, and the effect of different configurations of DNA is different.

Analysis of common problems Common problems Cause countermeasures DNA bands fuzzy DNA degradation experiment process to avoid nuclease contamination of the electrophoresis buffer Old Electrophoresis Buffer After repeated use, the ionic strength decreases, the pH value rises, and the buffering capacity weakens, which affects the electrophoresis effect. 2. EB is easily degraded under light.5-1. The countermeasures are: adjust the PCR system and PCR program, and find out the appropriate conditions. The electrophoretic conditions used are not suitable.

Therefore, comprehensively consider the use of TBE buffer. Dilute the DNA DNA chain with 20mM Nacl buffer solution. According to the size range of the separated DNA molecules, select a gel with an appropriate ratio, weigh an appropriate amount of agar powder, put it in a conical flask, and add an appropriate amount of TBE electrophoresis buffer. Irregular DNA band migration electrophoresis conditions are not suitable when the electrophoresis voltage is not more than 6V / CM, the temperature is <30 ° C, and the large DNA strand is electrophoresed, the temperature should be <15 ° C. 
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