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2. Inhale the cell culture supernatant into a centrifuge tube and centrifuge at 1000 × g for 20 minutes to remove cell debris and impurities. Carefully collect the supernatant (plasma). Tissue homogenate 1) Rinse the tissue with pre-chilled PBS (0. 5) Centrifuge at 10,000 × g for 10 minutes at 4 ° C to remove cell debris. Use a pyrogen-free and endotoxin-free test tube or centrifuge tube to collect blood samples, and place the test tube or centrifuge tube at room temperature for 2 hours or 4 ° C overnight to isolate the serum. So that the serum can be more separated).

In this case, non-specific color reaction will occur in ELISA experiments, resulting in inaccurate test results. Repeat the freeze-thaw process several times until the cells are completely lysed.. Pretreatment methods are different for different sample types. Proper sample preparation is the first step to ensure the correctness and accuracy of ELISA experiments. 6.

Samples should avoid hemolysis or hyperlipidemia. In a 6-well culture plate, 150-250 μL of PBS is required for each well to resuspend the pipette tips suppliers cells. Plasma Use blood collection tubes or centrifuge tubes containing anticoagulants to collect blood samples. 3. Hemolysis should be avoided during the collection of blood samples, because red blood cells will release substances with peroxidase activity when they are lysed. Centrifuge at 1000 × g for 15 minutes at 4 ° C for 30 minutes after collection. 3)

Resuspend cells by adding an appropriate amount of pre-chilled PBS (protease inhibitors are recommended immediately before use). Please read the instructions of the ELISA kit carefully before testing to see if the kit has special requirements for anticoagulants. Centrifuge at 1000 × g for 20 minutes at 4 ° C, carefully collect the supernatant (serum), and immediately perform the measurement. It is recommended to store the collected serum in aliquots at -20 ° C or -80 ° C to avoid repeated freeze-thaw cycles.01M, pH 7. The suspension can also be sonicated using an ultrasonic cell disruptor to lyse the cells. Cell culture supernatant. 4. 3)
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