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The 5ml Pipette tips medium needs to be clarified to facilitate the observation of the growth of bacteria. ④ Close the exhaust valve to increase the vapor pressure to the specified requirements and maintain it for the specified time (depending on the nature of the sterilized article and related circumstances). 3. 8. When inoculating food samples, special work clothes, caps and slippers must be worn. The door should be closed tightly before and after use in the sterile room, and the UV lamp should be turned on. If there is damage or the tampon is removed, it cannot be used as a sterile item.

(3) The bottle and bagged food should be opened with sterilization operation, and the above method should be selected for inoculation after treatment. At present, there are many kinds of dry culture media. 3. Some substances are easily destroyed by high-pressure steam sterilization. (2) Check whether the pressure gauge stays at zero when the steam is exhausted, close the door and cover, and then observe the use of the pressure cooker or vertical pressure steam sterilizer after the steam or heat is applied: ① Portable Add 3L of water to the main body of the pressure cooker, and add 16L of water to the vertical pressure cooker (the water should be replenished when it is reused, and the water becomes turbid and needs to be replaced).

A small window of 0. (2) Intermittent sterilization method 1. (8) After the completion of each batch of sterilization, record the name, quantity, temperature, time, and operator of the sterilization. Fifth, the preparation of culture media requires that the quality of culture media preparation will directly affect the growth of microorganisms.

The sterilization of the culture medium must not only achieve the purpose of complete sterilization, but also pay attention not to reduce its nutritional value due to heating. (2) Close the drain, open the intake valve, and disinfect for 10-20 minutes as required. VII. (2) Temperature and time of pressure steam sterilization. 2. If the indoor hanging UV lamp is used for disinfection, a 30W UV lamp is needed, the distance is 1. Weigh out the ingredients according to the formula of the culture medium, and then dissolve them in distilled water. The washing solution of the strong bacteria must be washed in the beaker. ②
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