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HEPES BUFFER CAS 7365 45 9

HEPES Wholesale

HEPES (4-Hydroxyethylpiperazineethanesulfonic acid) is a hydrogen ion buffer that can control a constant pH range for an extended period of time. It is often used to adjust the pH value of the reaction system in biochemical experiments, such as virus preservation solution or cell preservation solution, which contains this component. It has no toxic effect on cells, and can control a constant pH range for a long time. It can not only be used as a buffer in the medium, but also as a pH regulator in cosmetics.

HEPES Buffer in Detail
HEPES is a Good's buffer reagent, the full name is N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid. Its pH buffer range is in the range of 6.8 to 8.2. It is easily soluble in water, and there is no precipitation reaction with multivalent metal ions. In addition, most of the natural factors will not affect the dissociation of HEPES, such as temperature, concentration, medium salt and ion composition, etc. It can be seen that HEPES is very stable.

Applications of HEPES Powder
HEPES is often used in cell culture media of various types of organisms as buffer reagents, cell-cell adhesion, short-term cell aggregation culture, and buffers for tissue and cell cleaning;

HEPES is used in cosmetics: HEPES ha s the advantages of being safe to human body, non-toxic, good physiological compatibility and stable chemical properties. The anti-aging effect of the product lasts for a long time, so it is often used as a stabilizer and active agent in cosmetics.

How to use HEPES Buffer:
The first method of use is that HEPES is directly added to the prepared culture solution according to a certain concentration required, and then filtered and sterilized. Add 2.38g of HEPES buffer reagent to each thousand milliliters of culture solution, and adjust the pH to 7.2 with 1N sodium hydroxide after dissolving, and then use it normally after filtration and sterilization. At this time, the concentration of HEPES biological buffer is 10 mmol/L.

The second method of use usually takes 99 milliliters of culture solution before use, and then adds 1 milliliter of stock solution. At this time, the application concentration of this solution is still 10 mmol/L. Dissolve 23.8g of HEPES in 90ml of double-distilled water, adjust the pH to 7.5 to 8.0 with 1N sodium hydroxide, start to dilute to 100ml with water, filter and sterilize, divide into 2ml vials, and store at 4°C or -20 °C. This is how a 1 mol/L (100 x) HEPES stock solution is formulated.

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