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The name of HEPBS is 4-(4-(2-Hydroxyethyl)Piperazin-1-Yl)Butane-1-Sulfonic Acid). In terms of molecular structure, HEPBS is a zwitterionic compound with both positive and negative charges. It is commonly used as a buffer in cell culture media, cosmetic raw materials and diagnostic tests after titration with sodium hydroxide to adjust the acid-base balance of solutions. Since the HEPBS buffer is greatly affected by the pH value, when the pH value of the solution is 7.6-9.0, the buffering capacity of the HEPBS buffer is the greatest.

HEPBS Buffer Details

HEPBS and PIPES, HEPES, POPSO, HEPPS, HEPPSO, POPSO all contain piperazine rings in their chemical structures, and their chemical properties are very similar to their conventional uses. Biologically, this category is classified as a piperazine family buffer. This kind of buffer is chemically inactive and does not react with most metals to produce precipitates; it is very suitable for environmental research, chemical analysis and biological research, and can have redox reactions with strong oxidants and strong reducing agents. At 25°C, HEPBS has a pKa value of 8.3, which is the acidity value required for cell growth, so HEPBS is often used in cell culture media.

The UV absorbance of HEPBS buffer is very small. In 33%W/W sodium hydroxide solution, the UV absorbance of A290 is less than or equal to 0.1. The HEPBS produced by Desheng is a white crystalline powder at room temperature. When its moisture is measured by the Karl Fischer method, the loss on drying is less than or equal to 1%. After 0.5 g of HEPBS powder was completely dissolved in 1 ml of water, the solution was slightly yellow. Desheng's production process of HEPBS has been continuously improved, with a purity of more than 99%, stable quality and timely delivery.

HEPBS Buffer Advantages

HEPBS has high saturation, 0.5g powder can be completely dissolved in 1ml water;

The loss on drying is small. When the moisture is measured by the Karl Fischer method, the loss on drying is less than or equal to 1%.

At room temperature, the pKa value is high, which is 8.3;

The applicable PH value range is high, 7.6-9.0;

The HEPBS buffer has good stability, is not sensitive to changes in ambient temperature, and can keep the pH value of the solution in a stable state for a long time;

Adding non-isoionic inorganic salts to HEPBS buffer will not change the activity coefficient of the solution;

Under normal circumstances, HEPBS buffer will not penetrate the biofilm;

When irradiated with ultraviolet and visible light waves, the ability of HEPBS buffer to absorb light is very small;

HEPBS does not react with the enzyme to reduce the activity of the enzyme;

HEPBS cannot react with most metal ions and is suitable for cell culture experiments that require metal cations.

Advantages of Desheng on HEPBS Buffer

High purity, above 99%;

The quality is good, the HEPBS produced by our company is pure white crystalline powder, which has no effect on the biochemical reaction;

Can support HEPBS small batch customization service;

Advanced equipment and stable delivery;

The professional R&D team continuously studies the production process of HEPBS, and can adjust, improve and distribute samples according to customer requirements;

HEPBS sample testing and testing services can be provided according to customer requirements;

Can be shipped worldwide.

Application of HEPBS Buffer

As a piperazine series buffer, HEPBS can provide suitable acidity for cell culture medium. HEPBS buffer is chemically inactive and cannot precipitate with metal ions, so it is often used in cell culture media that require metal ions. HEPBS is also commonly used in cosmetic raw materials as a buffer to adjust the acidity and alkalinity of various cosmetic ingredients.

HEPBS Buffer Notes

HEPBS can react with strong acids/bases, strong oxidants/reducing agents, so it is not suitable for reduction-oxidation research, and should be kept away from these substances during storage;

The buffering capacity of HEPBS buffer is affected by temperature. Please store it at room temperature. Try to use it as soon as possible. Long-term storage is prone to contamination of impurities.

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