年表作成・共有サービス - pastport

https://www.hongvadar.com/threonine-production-line/

HongVadar company provides you the Lysine, Tryptophan, Threonine, Valine, Leucine, etc. fermentation products project. HongVader as agricultural processing plants play the roles of corn, sweet corn processing plant, potato processing plant, starch of corn and wheat processing plant, taking wheat, rice, cassava processing, msg project, and production lines of ethanol glucose, DMH, fructose, sorbitol. The production capacity is diversified from 10000 tons per year to 600000 tons per year.

The threonine production line supplied by HongVadar is based on Escherichia coli genetic engineering bacteria for pure-breed culturing and takes the physical separation to get wet threonine, then after trying to get qualified threonine product.

Threonine Production Line Process Description
Fermentation air pre-treatment
The location of the fresh air for air pretreatment should be far away from the cold water tower, the corn purification workshop, etc. And the location of the air inlet should be higher than 25 meters to avoid a place with high air humidity and dust. The fresh air is cooled and dehumidified and enters the air heat exchanger. The air is heated by steam as the heat source. The fresh air is processed into sterile and dry air by total filtration and membrane filtration.

Strain culture
The fermentation of the threonine protein is oxygen-consuming fermentation. Fermented strains for producing threonine protein were bred by directed mutagenesis and kept in the refrigerator at low temperatures. Expansion culture of strain should be carried out before fermentation inoculation in order to restore its vitality and achieve a good physiological state. Generally, the expanded culture of strain can be divided into three steps: slope culture, seed culture, and final fermentation.

Fermentation
There were bacteria, residual sugars, pigments, colloidal substances and other fermentation byproducts in the low threonine fermentation broth. Before fermentation, the fermentation broth needs to be heated to 65-75℃ for sterilization. After the fermentation, it is sent to the next stage of filtration and purification process.

Membrane filtration
The ceramic membrane was used to concentrate and filtrate the fermentation broth, and the dialysis water was used to reduce the threonine content in the concentrate solution, so as to reduce the loss caused by filtration of the ceramic membrane. The substance with molecules larger than the interception molecular weight of the membrane does not pass through the ceramic membrane and forms to bacterial protein, which is added to the mother liquor to produce protein feed product. The dialysate moves on to the next process.

Evaporation crystallization
The dialysate enters the multi-effect evaporation crystallizer to concentrate and crystallize the material. The feed concentration of the evaporator is 12-13g/100ml. After the concentration and crystallization, the solid-liquid ratio of the discharge reaches 58-60% and enters the separation process. The evaporated condensed water is for fermentation liquid blending, steam condensate is recycled by the power plant to save cost.

Centrifugal separation
The crystal slurry is evenly distributed to the first drum through the distributor by the feed pipe of the separator. The liquid part moves in a circle with the drum. The centrifugal force generated will collect the mother liquid to the mother liquid collection tank and discharge it to the mother liquid storage tank. The crystal part is deposited on the screen to form the filter cake. The filter cake is pushed into the second-stage drum by the reciprocating motion of the first-stage drum. When the filter cake is pushed to the outlet, the crystal threonine with very low water content is obtained under greater centrifugal force.

Fluidized bed drying system
The Crysta L-threonine is transferred into the fluidized bed dryer through the vibrating screen, and the crystal water of the Crysta L-threonine is dried by hot blast and draught air. After multi-stage screening treatment, the uniform particle threonine products are obtained.