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https://www.metwarebio.com/plant-hormone-targeted-metabolomics-service/

Phytohormone is also known as plant natural hormone or plant endogenous hormone. It refers to some trace organic compounds produced in plants that can regulate (promote or inhibit) their own physiological processes. Based on LC-MS/MS technology, Metware Biotechnology has developed a professional method for the plant hormone detection, covering 108 kinds, such as ABA, Auxin, CK, Ethylene, GA, JA, SA and SL.

Benefits of Plant Hormone Analysis, Plant Hormone Assay
1. Complete variety:

108 kinds of plant hormones can be detected.

2. High sensitivity:

Using AB Sciex QTRAP 6500
LC-MS platform, The sensitivity can reach ng/mL level.

3. Rich experience:

More than 2000 project experiences
covering 500+ species, and have been published in Cell, Nature Communications, The Plant Journal and other journals.

4. Quantitative accuracy:

External standard + internal standard
method, linear relationship of the standard curve is above 0.99.

Applications of Phytohormone Assay
Research on pant growth, reproduction and development (differentiation, maturation, aging, apoptosis etc.) ;

Abiotic stress research (drought resistance, cold resistance, etc.)
;
Biological stress response research (disease and insect resistance, etc);

Plant hormone interactions.

Plant Hormone Assay FAQs

What are the methods to detect plant hormones? What are the differences between them?

liquid chromatography (HPLC), and liquid mass spectrometry (HPLC-MS).

ELISA method: The enzyme immunoassay for phytohormone metabolomics mainly consists of encapsulated antibodies on solid phase carriers (direct method) and encapsulated antigens (indirect method). The direct method uses free antigens and enzyme-labeled antigens to compete with adsorbed antibodies, and the indirect method uses free antigens and adsorbed antigens to compete with free antibodies.

High performance liquid Chromatography (HPLC): using liquid as the mobile phase and a high-pressure infusion system, the separation is achieved by using the small differences in the adsorption or partition coefficients of substances in the two phases.

Liquid mass spectrometry (HPLC-MS): also called liquid chromatography-mass spectrometry (LC-MS), fully combines the high separation ability of HPLC and the strong qualitative ability of MS. After the sample is separated in the chromatography, it enters the mass spectrometry, which analyzes the mass-to-charge ratio of the measured sample ions.
Detection methodPre-treatment requirementsDifficulty of operationDetection throughputCostsInstructions
ELISALowEasyLargeLowSuitable for large number of samples with low requirements for data accuracy
HPLCHighDifficultSmallHighSuitable for high content of IAA, ABA, SA and other hormones, but not for low content of GA, SL, JA, BR and other hormones
HPLC-MSVery highExtremely DifficultSmallVery highThe detection accuracy can reach ng level, which is suitable for customers who require high data accuracy and high score articles

If you want to know more about plant hormone targeted metabolomics, please visit our website.

More details of metabolome project, please contact us.