Quantitative Lipidomics for Plants

(2023年01月17日)

https://www.metwarebio.com/quantitative-lipidomics-service-for-plants/

Metware Biotechnology uses Widely Targeted Metabolomics technology to build a database specific for plant lipids and performs qualitative analysis from the mass spectrometry data of many biological materials, followed by absolute quantification using QqQ and MRM to provide a comprehensive analysis of lipids in plant samples. This process is applicable to many plant research questions in growth and development as well as stress response.

Technology Introduction of Quantitative plant lipidomics
A class of organic compounds known as lipids are easily soluble in non-polar solvents but insoluble in water. The lipidome, as a subtype of metabolome, focuses on annotating and quantifying lipids in samples in order to understand their functions in plant growth and development. Important cellular processes, such as material transport, energy metabolism, information transfer, and metabolic regulation, all depend on lipids. With the help of Widely Targeted Metabolomics and a specific database of more than 2500 plant lipids, our Quantitative Lipidomics Service for Plants allows high throughput analysis of plant lipids in all types of plant materials.

Technical Features of Quantitative Lipidomics Service for Plants

Detection Platform
LC-MS for metabolite detection, both qualitative and quantitative accuracy.

Comprehensive Database
Proprietary metabolite database for plant species, with 2500+ lipid compounds in the database.

Rich Variety of Substances
Covering 6 major types of fatty acyl, glycerol esters, glycerophospholipids, sphingolipids, sterol esters, and isopentenol lipids.

Stable Detection
10 major quality control processes for providing high quality data.

Leading in the Number of Detected Substances
Between 800-1000 lipids detected in a typical run.

Comprehensive Analysis
Service comes with 12 analysis content and 22 display figures.

Post-sale Support
Comprehensive interpretation of the final report.

Applications of Plant Quantitative Lipidomics Service
Plant growth and development.

Abiotic and biotic stresses.

Food quality and processing.

Analysis Cases Display of Plant Quantitative Lipidomics Service
Journals: Plant Physiology;

IF: 6.902

Figure 1: Metabolomic analysis of primary metabolites in axillary buds of 35S: BnFAX6 transgenic plants and decapitated WT plants of B. napus.

Sample Requirements of Plant Quantitative Lipidomics Service

Sample requirements
Sample TypesMinimum sample sizeRisk minimum sample sizeBiological Repetition
TissueStems, buds, nodes, leaves, flowers, fruits, roots, healing tissues600mg300mg3-6
LiquidRoot secretions10mL3mL
Fermentation fluid, wine, tissue fluid, juice5mL3mL
Honey, nectar, oil, herbal decoction, extracts500μL300μL
SpecialCultured samples, present liquids600mg300mg
Note: Packed into suitable size centrifuge tubes (10mL/15mL), snap frozen in liquid nitrogen for 5-10min, stored in -80℃ refrigerator and transported on dry ice.

There are many metabolomics service providers, but we are one of the best choices for you.

quantitative-lipidomics-service-for-plants.jpg

コメント